Chemical Name: N-(8-Maleimidocaproyloxy)sulfosuccinimide, sodium salt
Appearance: white or slightly yellowish-pink powder
Purity: ≥90.0% (HPLC)
MW: 438.39, C16H19N2NaO9S
Storage Condition: 0-5ºC, protect from moisture
Shipping Condition: blue ice
Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature since it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Heterobifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.
Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent
1. J. V. Staros, N-Hydroxysulfosuccinimide
Active Esters:Bis(N-hydroxysulfosuccinimide) Esters of Two Dicarboxylic
acids Are Hydrophilic, Membraneimpermeant, Protein Cross-linkers,
Biochemistry, 1982, 21, 3950.
2. P. S. R. Anjaneyulu, James V.
Staros, Reactions of N-Hydroxysulfosuccinimide Active Esters, Int. J.
Peptide Protein Res., 1987, 30, 117.
3. Y. Fukami, K. Sato, K.
Ikeda, K. Kamisango, K. Koizumi and T. Matsuno, Evidence for
Autoinhibitory Regulation of the c-src Gene Product a Possible
Interaction Between the Src Homology 2 Domain and Autophosphorylation
Site, J. Biol. Chem., 1993, 268, 1132.