Reductive Chromogenic Dye
Product codeT012 TB
Chemical name3,3'-[3,3'-Dimethoxy-(1,1'-biphenyl)-4,4'-diyl]bis(2,5-diphenyl-2H-tetrazolium chloride)
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TB is slightly soluble in water, but does not dissolve easily in acetone, ethyl acetate, and ether.
Other organic solvents such as methanol, ethanol, and tetrachloromethane can solubilize TB pretty well.
Since TB is easily reduced with dehydrogenase to generate reddish-purple formazan dye aggregates, it is used for dehydrogenase detection in tissue samples.
1. K. Tsou, et al., Synthesis of Some p-Nitrophenyl Substituted Tetrazolium Salts Electron Acceptors for the Demonstration of Dehydrogenases. J Am Chem Soc. 1956;78:6139-6144.
2. S. S. Karmarkar, et al., Preparation of Nitrotetrazolium Salts Containing Benzothiazole. J Org Chem. 1960;25:575-578.
3. J. E. Sinsheimer, et al., Reactivity of Blue Tetrazolium with Nonketol Compounds. Anal Chem. 1965;37:566-569.
Handling and storage condition
|Appearance：||Pale yellow to pale yellowish orange powder|
|Solubility in water：||To pass test (clear, yellow)|
|Solubility in Methyl alcohol：||To pass test (clear, yellow)|
|m.p.：||≧ 248℃ (dec.)|
|Molar absorptivity：||≧ 52,500 (around 254 nm)|
|Sensitivity：||To pass test|