10 Redox Dyes

SAT-3

SAT-3

Oxidative Chromogenic Dye

  • Product code
    S302  SAT-3
  • CAS No.
    679787-07-6
  • Chemical name
    N,N'-Bis(2-hydroxy-3-sulfopropyl)tolidine, disodium salt, tetrahydrate
  • MW
    C20H26N2Na2O8S2・4H2O=604.62
Unit size Price Item Code
1 g $238.00 S302-10
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Oxidation Reaction

Product Description

SAT-3 is a stable, highly water-soluble o-Tolidine analog.

It is easily oxidized by peroxidase and hydrogen peroxide into a green dye at pH 4-6.

While TMBZ requires an organic solvent or detergent to solubilize, SAT-3 can be solubilized with just buffer solution.

The SAT-3 solution can be stored at room temperature with protection from light.

The maximum wavelength of the dye is 675 nm.

Using the Ames test, Nno mutagenicity of SAT-3 was detected. using the Ames test.

Technical info

1. Mix 1 ml of 100 mM SAT-3 solution and 1 ml of 30 mM hydrogen peroxide solution with 8 ml of 50 mM citrate buffer (pH 4) to prepare an assay solution.
2. Add 100-200 μl of the assay solution to each well of a 96-well plate and incubate at 37ºC for 5-30 minutes.
3. Read the O.D. at 670 nm or add 50 μl of 4 M sulfuric acid to each well and read the O.D. at 490 nm instead.

References

Open References

1) M. Mizoguchi, M. Ishiyama, M. Shiga and K. Sasamoto, "Sensitive Chromogenic Substrate for Detecting Peroxidase Activity", Anal. Commun199835, 179.

Handling and storage condition

Specification
Appearance: White to pale brown powder
Purity (HPLC): ≧95.0%
Solubility in water: To pass test (clear, colorless or slightly blue)
pH(25°C): 6.0~8.0 (25℃)
Water content: 9.0~14.0%
IR spectrum: Authentic
Handling and storage condition
0-5°C, Protect from light
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Product Classification

Product Classification

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