Oxidative Chromogenic Dye
Product codeS302 SAT-3
Chemical nameN,N'-Bis(2-hydroxy-3-sulfopropyl)tolidine, disodium salt, tetrahydrate
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SAT-3 is a stable, highly water-soluble o-Tolidine analog.
It is easily oxidized by peroxidase and hydrogen peroxide into a green dye at pH 4-6.
While TMBZ requires an organic solvent or detergent to solubilize, SAT-3 can be solubilized with just buffer solution.
The SAT-3 solution can be stored at room temperature with protection from light.
The maximum wavelength of the dye is 675 nm.
Using the Ames test, Nno mutagenicity of SAT-3 was detected. using the Ames test.
1. Mix 1 ml of 100 mM SAT-3 solution and 1 ml of 30 mM hydrogen peroxide solution with 8 ml of 50 mM citrate buffer (pH 4) to prepare an assay solution.
2. Add 100-200 μl of the assay solution to each well of a 96-well plate and incubate at 37ºC for 5-30 minutes.
3. Read the O.D. at 670 nm or add 50 μl of 4 M sulfuric acid to each well and read the O.D. at 490 nm instead.
1) M. Mizoguchi, M. Ishiyama, M. Shiga and K. Sasamoto, "Sensitive Chromogenic Substrate for Detecting Peroxidase Activity", Anal. Commun, 1998, 35, 179.
Handling and storage condition
|Appearance：||White to pale brown powder|
|Solubility in water：||To pass test (clear, colorless or slightly blue)|
|0-5°C, Protect from light|