Product codeS250 Sulfo-KMUS
Chemical nameN-(11-Maleimidoundecanoyloxy)sulfosuccinimide, sodium salt
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Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature since it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Heterobifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.
Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent
1) J. V. Staros, "N-Hydroxysulfosuccinimide Active Esters:Bis(N-hydroxysulfosuccinimide) Esters of Two Dicarboxylic acids Are Hydrophilic, Membrane-impermeant, Protein Cross-linkers", Biochemistry, 1982, 21, 3950.
2) P. S. R. Anjaneyulu and James V. Staros, "Reactions of N-Hydroxysulfosuccinimide Active Esters", Int. J. Peptide Protein Res., 1987, 30, 117.
3) Y. Fukami, K. Sato, K. Ikeda, K. Kamisango, K. Koizumi and T. Matsuno, "Evidence for Autoinhibitory Regulation of the c-src Gene Product a Possible Interaction Between the Src Homology 2 Domain and Autophosphorylation Site", J. Biol. Chem., 1993, 268, 1132.
Handling and storage condition
|Appearance：||White to slightly yellowish pink powder|
|Purity (HPLC)：||≧ 90.0 %|
|Solubility in water：||To pass test (clear, slightly yellow)|