09 Cross-Linking Reagents

Sulfo-EMCS

Sulfo-EMCS

Cross-Linker

  • Product code
    S024  Sulfo-EMCS
  • CAS No.
    103848-61-9(free acid)
  • Chemical name
    N-(6-Maleimidocaproyloxy)sulfosuccinimide, sodium salt
  • MW
    C14H15N2NaO9S=410.33
Unit size Price Item Code
50 mg $387.00 S024-10
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Description

Cross-linking Reaction

 

Product Description
Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Hetero-bifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.

Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent

References

Open References

1) J. V. Staros, "N-Hydroxysulfosuccinimide Active Esters:Bis(N-hydroxysulfosuccinimide) Esters of Two Dicarboxylic acids Are Hydrophilic, Membrane-impermeant, Protein Cross-linkers", Biochemistry, 198221, 3950.
2) P. S. R. Anjaneyulu and James V. Staros, "Reactions of N-Hydroxysulfosuccinimide Active Esters", Int. J. Peptide Protein Res.198730, 117. 
3) Y. Fukami, K. Sato, K. Ikeda, K. Kamisango, K. Koizumi and T. Matsuno, "Evidence for Autoinhibitory Regulation of the c-src Gene Product a Possible Interaction Between the Src Homology 2 Domain and Autophosphorylation Site", J. Biol. Chem.1993268, 1132. 

Handling and storage condition

Specification
Appearance: White to slightly yellowish pink powder
Purity (HPLC): ≧ 90.0 %
Solubility in water: To pass test (clear, colorless to slightly yellow)
IR spectrum: Authentic
NMR spectrum: Authentic
Handling and storage condition
0-5°C
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Product Classification

Product Classification

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