19 Biochemicals



Colorimetric Reagent for SH Moiety

  • Product code
    P017  4-PDS
  • CAS No.
  • Chemical name
  • MW
Unit size Price Item Code
1 g $187.00 P017-10

1 g
Quantity may be adjusted within the cart.


Product Description
2-PDS and 4-PDS are used for the photometric determination of thiol groups in biological samples. These compounds react with the thiol groups at pH 5 to form mercaptopyridine (Fig. 1 and 2). The maximum wavelengths of 2-Mercaptopyridine and 4-Mercaptopyridine are 343 nm and 324 nm, respectively.

Fig 1. 4-PDS reaction with thiol compound


Open References

1) D. R. Grassetti and J. F. Murray Jr., "Determination of Sulfhydryl Groups with 2, 2'- or 4,4'-Dithiodipyridine", Arch. Biochem. Biophys., 1967, 119, 41.
2) R. E. Humphrey, M. H. Ward and W. Hinze, "Spectrophotometric Determination of Sulfite with 4,4'-Dithiodipyridine and 5,5'-Dithiobis-(2-nitrobenzoic acid)", Anal. Chem., 1970, 42, 698.
3) R. E. Humphrey and W. Hinze, "Spectrophotometric Determination of Cyanide with Organic Disulphides", Talanta, 1971, 18, 491.
4) T. Uete, Y. Miyamoto, M. Ohnishi and N. Shimano, "Spectrophotometric Micromethod for Measuring Cholinesterase Activity in Serum or Plasma", Clin. Chem., 1972, 18, 454.
5) A. Fago, C. Hundahl, S. Dewilde, K. Gilany, L. Moens and R. E. Weber, "Allosteric Regulation and Temperature Dependence of Oxygen Binding in Human Neurogobin and Cytoglobin", J. Biol. Chem., 2004, 279(43), 44417.

Handling and storage condition

Appearance: White to pale yellow crystalline powder
Solubility in Methyl alcohol: To pass test (clear, colorless or slightly yellow)
Molar absorptivity: ≧ 15,000 (around 246 nm)
IR spectrum: Authentic
m.p.: 73 - 78oC
Sulfated ash: ≦ 0.10 %
Handling and storage condition
Ambient temperature

Product Classification

Product Classification

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