10 Redox Dyes

TOOS

TOOS

Trinder Reagent

  • Product code
    OC13  TOOS
  • CAS No.
    82692-93-1(anhydrous)
  • Chemical name
    N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline, sodium salt, dihydrate
  • MW
    C12H18NNaO4S・2H2O=331.36
Unit size Price Item Code
1 g $116.00 OC13-10
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Description

Oxidation Reaction

Technical info

Preparation of Assay Solution – TOOS
1. Dissolve 20 mg TOOS with 10 ml PBS to prepare 6.6 mM TOOS solution.
2. Dissolve 14 mg 4-aminoantipyrin (AA) with 10 ml PBS to prepare 6.6 mM 4-AA solution.
3. Prepare 2 U/ml horseradish peroxidase solution with PBS.
4. Mix the same volume of each solution together to prepare assay solution. Store the assay solution at 4°C with protection from light.

 

Assay Protocol
1. Prepare sample solutions for the enzymatic oxidation reaction. The pH range of the buffer solution should be from 5.5-9.5.
2. Prepare standard solutions containing known amounts of substrate using the same buffer.
3. Add the appropriate units of oxidase to the sample solution, followed by an addition of the same volume of the assay solution.
4. Incubate the mixture at room temperature or at 37oC for 30 min to 1 hour.
5. Measure the O.D. at 555 nm.
6. Prepare a standard curve, and determine the substrate concentration in the sample solution.

References

Open References

1. K. Tamaoku, et al., New water-soluble Hydrogen Donors for the Enzymatic Photometric Determination of Hydrogen Peroxide. II. N-Ethyl-N-(2-hydroxy-3-sulfopropyl)aniline derivatives. Chem Pharm Bull. 1982;30:2492-2497.
2. K. Tamaoku, et al., New water-soluble Hydrogen Donors for the Enzymatic Spectrophotometric Determination of Hydrogen Peroxide. Anal Chim Acta. 1982;136:121-127.
3. B. C. Madsen, et al., Flow Injection and Photometric Determination of Hydrogen Peroxide in Rainwater with N-Ethyl-N-(sulfopropyl)aniline sodium salt. Anal Chem. 1984;56:2849-2850.

Handling and storage condition

Specification
Appearance: White to slightly blue powder or crystalline powder
Purity (Absorbance): ≧ 98.0 %
Solubility in water: o pass test (clear, colorless or slightly yellow green) Absorbance (600 nm)≦ 0.015 Absorbance (400 nm)≦ 0.040
pH(25°C): 8.5 - 9.5 (25℃)
Water content: 9.0 - 12.0 %(K. Fischer)
Heavy metals (as Pb): ≦ 0.0005 %
Iron (Fe): ≦ 0.0005 %
IR spectrum: Authentic
Handling and storage condition
Protect from light
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Product Classification

Product Classification

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