10 Redox Dyes

Nitro-TB

Nitro-TB

Reductive Chromogenic Dye

  • Product code
    N011  Nitro-TB
  • CAS No.
    298-83-9
  • Chemical name
    3,3'-[3,3'-Dimethoxy-(1,1'-biphenyl)-4,4'-diyl]-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]
  • MW
    C40H30Cl2N10O6=817.64
Unit size Price Item Code
100 mg $42.00 N011-10
1 g $225.00 N011-12
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Reaction Scheme

Product Description

Nitro-TB is dissolved by hot water or hot methanol. Other organic solvents such as acetone and ether cannot solubilize Nitro-TB. Nitro-TB is easily reduced by dehydrogenase to purple formazan dye aggregates. It is used for dehydrogenase detection on agarose gels.

References

Open References

1) K-C. Tsou, C-S. Cheng, M. M. Nachlas and A. M. Seligman, "Synthesis of Some p-Nitrophenyl Substituted Tetrazolium Salts Electron Acceptors for the Demonstration of Dehydrogenases", J. Am. Chem. Soc., 1956, 78, 6139.
2) J. R. Baker, D. V. Zyzak, S. R. Thorpe and J. W. Baynes, "Mechanism of Fructosamine Assay: Evidence against Role of Superoxide as Intermediate in Nitroblue Tetrazolium Reduction", Clin. Chem., 1993, 39, 2460.

Handling and storage condition

Specification
Appearance: Yellow crystalline powder
Purity (Absorbance): ≧ 98.0 %
Solubility in water: To pass test (clear, pale yellow or yellow)
Solubility in Methyl alcohol: To pass test (clear, pale yellow or yellow)
Solubility in Tris buffer: To pass test (clear, pale yellow or yellow)
Molar absorptivity: ≧ 63,000 (around 257 nm)
Water content: ≦ 5.0 %
Sensitivity: To pass test
Loss on drying (110 °C): ≦ 9.5 %
Sulfated ash: ≦ 0.20 %
IR spectrum: Authentic
Thin Layer Chromatogram: Correspond to standard Nitro-TB
Handling and storage condition
Ambient temperature
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Product Classification

Product Classification

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