Ab-10 Rapid Peroxidase Labeling Kit

Ab-10 Rapid Peroxidase Labeling Kit

Antibody Labeling

  • Small Quantity of Antibody Requirement : 10 ug Antibody Sample Labeling
  • Easy Labeling Procedure : Just Mix Antibody and Labeling Agent
  • Rapid Labeling : Less than 30 minutes
  • Product code
    LK33  Ab-10 Rapid Peroxidase Labeling Kit
Unit size Price Item Code
3 samples $202.00 LK33-10
ORDER
Component
3 samples ・Reactive Peroxidase
・Reaction Buffer
・Stop Solution
x3
100 μlx1
100 μlx1

Description

Ab-10 Rapid Peroxidase Labeling Kit is for labeling peroxidase to 10 µg antibody in less than 30 minutes. Labeling agent included in the kit has succinimidyl ester group , which can easily make a covalent bond with an amino group of the target antibody without any activation process. The kit contains all the necessary reagents for antibody labeling.

Fig. 1 IgG Labeling Reaction of NH2-reactive Peroxidase

Fig. 2 Labeing Procedure

Developer Dojindo Molecular Technologies, Inc.

Manual

Technical info

♦ Use 0.5-1 mg/ml of antibody solution for labeling. If the antibody concentration is higher than 1mg/ml, dilute the antibody solution with PBS.
♦ If the sample solution contains small insoluble materials, centrifuge the solution, and use the supernatant for the labeling.
♦ The microtube in this kit contain solutions. Since there is a possibility that the droplets might be attached to the inside walls or caps, please centrifuge to drop the droplets prior to opening.
♦ Some additives in antibody solution may interfere with the labeling if the concentration is too high. The maximum compatible concentrations of such additives are indicated on Table 1.
♦ After a Reactive Fluorescent is removed from the seal bag, keep the unused Reactive Fluorescein in the bag, seal tightly and store at -20ºC.Store the other components at 0-5ºC.
♦ Since reactive reagent binds to an amino group in antibody, there is a possibility that the labeled antibody loses the antigen recognition ability (Table 2).

Table. 1 Compatible concentrations of the additives

Table. 2 Non-Compatible Antibody

Experimental Data

Fig. 3 Microscope Image of Mitochondria in Hela Cells

Peroxidase were to anti-mitochondria antibody using Ab-10 Rapid Peroxidase Labeling Kit (code:LK33)

References

Open References

1) A. R. A Moghani, M. K. Sharma and Y. Matsumoto, In cellulo phosphorylation of DNA double-strand break repair protein XRCC4 on Ser260 by DNA-PK.", J. Radiat. Res.., 2018, 59, 700.
2) K. Hitachi, M. Nakatani, A. Takasaki, Y. Ouchi, A. Uezumi, H. Ageta, H. Inagaki, H. Kurahashi and  K. Tsuchida, "Myogenin promoter-associated lncRNA Myoparr is essential for myogenic differentiation..", EMBO Rep., 2019, 20, (3), doi: 10.15252/embr.201847468.

Handling and storage condition

Handling and storage condition
0-5°C, Protect from moisture
Contact
Price

Product Classification

Product Classification

Search word