D-Luciferin K salt
Substrate for Luciferase
Product codeL226 D-Luciferin K salt
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D-Luciferin is a firefly luciferase substrate. Its quantum efficiency is 0.88, which is 20 times that of Luminol. The reaction mechanism is shown below. First, Luciferin reacts with ATP by luciferase in the presence of magnesium ion, then it is oxidized to form dioxetane structure and emits yellow-green light. Luciferin-luciferase luminescence is used for ATP monitoring to determine cell viability and bacteria counting. It is also used for reporter gene assays.
1. V. T. Nguyen, et al., Firefly Luciferase Luminescence Assays Using Scintillation Counters for Quantitation in Transfected Mammalian Cells. Anal Biochem. 1988;171:404-408.
2. S. P. Crouch, et al., The Use of ATP Bioluminescence as a Measure of Cell Proliferation and Cytotoxicity. J Immunol Methods. 1993;160:81-88.
3. S. R. Ford, et al., Improvements in the Application of Firefly Luciferase Assays. Methods Mol Biol. 1998;102:3-20.
Handling and storage condition
|Appearance：||Pale yellow to yellow powder|
|Purity (HPLC)：||≧ 98.0 %|
|Solubility in water：||To pass test (clear, slightly to pale yellow)|
|-20°C, Protect from light, Nitrogen substitution, Protect from moisture|