-Cellstain- DAPI solution
Dead Cell Staining
Product codeD523 -Cellstain- DAPI solution
Chemical name4',6-Diamidino-2-phenylindole, dihydrOchloride, solution
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DAPI is an AT-sequence specific DNA intercalator that attaches to DNA at the minor groove of the double helix like Hoechst dyes. Though DAPI is not permeable through viable cell membranes, it passes through disturbed cell membranes to stain the nucleus. DAPI has a high photo-bleaching tolerance level. DAPI is utilized for the detection of mitochondrial DNA in yeast, chloroplast DNA, virus DNA, micoplasm DNA and chromosomal DNA. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.
1.Prepare 10-50 μM DAPI solution with PBS or an appropriate buffer.a)
2.Add DAPI solution with 1/10 of the volume of cell culture medium to the cell culture.b)
3.Incubate the cell at 37oC for 10-20 min.
4.Wash cells twice with PBS or an appropriate buffer.
5.Observe the cells using a fluorescence microscope with 360 nm excitation and 460 nm emission filters.
a) Since DAPI may be carcinogenic, extreme care is necessary during handling.
b) Or you may replace the culture medium with 1/10 concentration of DAPI buffer solution.
Fig. 1 Cell staining with DAPI
1) W. Schnedl, A. V. Mikelsaar, M. Breitenbach and O. Dann, "DIPI and DAPI: Fluorescence Banding with Only Negligible Fading", Hum. Genet., 1977, 36, 167.
2) I. W. Taylor and B. K. Milthorpe, "An Evaluation of DNA Fluochromes, Staining Techniques, and Analysis for Flow Cytometry. I. Unperturebed Cellpopulations", J. Histochem. Cytochem., 1980, 28(11), 1224.
3) F. Otto and K. G. Tsou, "A Comparative Study of DAPI, DIPI, and Hoechst 33258 and 33342 as Chromosomal DNA Stains", Stain Technol., 1985, 60, 7.
4) N. Poulin, A. Harrison and B. Palcic, "Quantitative Precision of an Automated Image Cytometric System for the Measurement of DNA Content and Distribution in Cells Labeled with Fluorescent Nucleic Acid Stains", Cytometry, 1994, 16, 227.
5) M. Kawai, N. Yamaguchi and M. Nasu,"Rapid Enumeration of Physiologically Active Bacteria in Purified Water Used in the Pharmaceutical Manufacturing Process", J. Appl. Microbiol., 1999, 86 (3), 496.
6) Y. Saito, T. Chikenji, T. Matsumura, M. Nakano and M. Fujimiya, "Exercise enhances skeletal muscle regeneration by promoting senescence in fibro-adipogenic progenitors", Nat. Commun., 2020, doi:10.1038/s41467-020-14734-x.
Handling and storage condition
|Appearance：||Slightly yellow to yellow liquid|
|Dye content：||To pass test|
|0-5°C, Protect from light|