15 Chromogen/Metal Indicator



Metal Indicator

  • Product code
    D027  DAN
  • CAS No.
  • Chemical name
  • MW
Unit size Price Item Code
1 g $364.00 D027-10
1 g
Quantity may be adjusted within the cart.

Product Description

DAN is a highly selective colorimetric and fluorometric reagent for Se detection. This reagent is almost insoluble in cold water and alcohol, but it is higly soluble in water at 50ºC. DAN forms 4,5-benzopiaselenol by the complex with Se, and this complex is extracted with chloroform, toluene, or cyclohexane. Since the Se complex is volatile, Se can be determined by gas chromatography. Further, DAN generates fluorescent naphthalenetriazole by the reaction with nitrite ion, so it is also used for the fluorometric determination of nitrite.

Chemical Structure

Technical info

Solvent extraction, colorimetric: Se(IV) (in toluene at pH 1.2-2.2, λmax=380 nm, ε=11,800, detection range 0-4 ppm)
Solvent extraction, fluorometric: Se(IV) (λem=520 nm in toluene, λem=538 nm in cyclohexane, detection range 1-100 ppb)


Open References

1. J. H. Wiersma, 2,3-Diaminonaphthalene as a Spectrophotometric and Fluorometric Reagent for the Determination of Nitrite Ion. Anal Lett. 1970;3:123-132.
2. G. L. Wheeler, et al., Rapid Determination of Trace Amounts of Selenium(IV), Nitrate, and Nitrate by High-pressure Liquid Chromatography Using 2,3-Diaminonaphthalene. Microchem J. 1974;19:390-405.
3. E. M. Rodriguez, et al., Critical Study of Fluorimetric Determination of Selenium in Urine. Talanta. 1994;41:2025-2031.
4. A. M. Miles, et al., Fluorometric Determination of Nitric Oxide. Methods. 1995;7:40-47.

Handling and storage condition

Appearance: White to pale yellowish brown powder
Solubility in Dilute hydrochloric acid: To pass test (clear, colorless to slightly pink)
m.p.: 185 - 200°C
IR spectrum: Appearance Solubility in 0.1 mol/l HCl solution (50 mg / 100 ml) m.p. IR spectrum White to pale yellowish brown powder To pass test (clear, colorless to slightly pink) 185 - 200oC Authentic
Handling and storage condition
-20°C, Protect from light

Product Classification

Product Classification

Search word