Product codeD027 DAN
|Unit size||Price||Item Code|
DAN is a highly selective colorimetric and fluorometric reagent for Se detection. This reagent is almost insoluble in cold water and alcohol, but it is higly soluble in water at 50ºC. DAN forms 4,5-benzopiaselenol by the complex with Se, and this complex is extracted with chloroform, toluene, or cyclohexane. Since the Se complex is volatile, Se can be determined by gas chromatography. Further, DAN generates fluorescent naphthalenetriazole by the reaction with nitrite ion, so it is also used for the fluorometric determination of nitrite.
Solvent extraction, colorimetric: Se(IV) (in toluene at pH 1.2-2.2, λmax=380 nm, ε=11,800, detection range 0-4 ppm)
Solvent extraction, fluorometric: Se(IV) (λem=520 nm in toluene, λem=538 nm in cyclohexane, detection range 1-100 ppb)
1. J. H. Wiersma, 2,3-Diaminonaphthalene as a Spectrophotometric and Fluorometric Reagent for the Determination of Nitrite Ion. Anal Lett. 1970;3:123-132.
2. G. L. Wheeler, et al., Rapid Determination of Trace Amounts of Selenium(IV), Nitrate, and Nitrate by High-pressure Liquid Chromatography Using 2,3-Diaminonaphthalene. Microchem J. 1974;19:390-405.
3. E. M. Rodriguez, et al., Critical Study of Fluorimetric Determination of Selenium in Urine. Talanta. 1994;41:2025-2031.
4. A. M. Miles, et al., Fluorometric Determination of Nitric Oxide. Methods. 1995;7:40-47.
Handling and storage condition
|Appearance：||White to pale yellowish brown powder|
|Solubility in Dilute hydrochloric acid：||To pass test (clear, colorless to slightly pink)|
|m.p.：||185 - 200°C|
|IR spectrum：||Appearance Solubility in 0.1 mol/l HCｌ solution (50 mg / 100 ml) m.p. IR spectrum White to pale yellowish brown powder To pass test (clear, colorless to slightly pink) 185 - 200oC Authentic|
|-20°C, Protect from light|