Mitochondria Research




Mitophagy
Reagent Mtphagy Dye Keima-Red
Principle Mtphagy Dye (included in Mitophagy Detection Kit) is a pH-sensitive fluorescent probe that accumulates in mitochondria and emits red fluorescence due to acidic conditions in a lysosome. A pH-sentitive ratiometric fluorescent protein. The excitation spectrum changes accoring to pH. This protein shows high fluorescence ratio (Ex. 550 nm/440 nm) values in a lysozome.
Fixed cell staining - -
Live-cell staining Yes Yes
Fixation after live-cell staining - -
Staining time > 30 min -
Ex / Em 530 / 700 440, 550 / 620
Product code MD01 , MT02 -



Lipophilic peroxide Singlet oxygen Superoxide
Reagent MitoPeDPP Si-DMA MitoSOX
Principle A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with lipophilic peroxides in mitochondria to emit fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with singlet oxigen generated in mitochondria to emit red fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit red fluorescence.
Fixed cell staining - - -
Live-cell staining Yes Yes Yes
Fixation after live-cell staining - - -
Staining time > 15 min > 45 min > 10 min
Ex / Em 452 / 470 644 / 670 510 / 590
Product code M466 MT05 -



Membrane potential Iron ion (Fe2+) Calcium ion (Ca2+)
Reagent JC-1 TMRM, TMRE Mito-FerroGreen Rhod2-AM
Principle A cell-permeant ratiometric fluorescent dye that accumulates in intact mitochondria due to the membrane potential. The excitation spectrum changes according to the mitochondria membrane potential. Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Diffusion of the probes occurs in a damaged mitochondria that has the decreased membrane potential. A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with ferrous ion in mitochondria to emit green fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with calcium ion in mitochondria to emit red fluorescence.
Fixed cell staining - - - -
Live-cell staining Yes Yes Yes Yes
Fixation after live-cell staining - - - -
Staining time 10-60 min 30-60 min 30 min 30-60 min
Ex / Em Monomer: 514 / 529
J-aggregation: 585/590
550 / 575 505 / 535 553 / 576
Product code MT09 - M489 R002



Mitochondria staining
Reagent MitoBright LT series MitoTracker series DsRed MitoRed Rhodamine 123
Principle Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. A red fluorescent protein that binds to intact mitochondria with the use of a transfection reagent. Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential. Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential.
Fixed cell staining - - - - -
Live-cell staining Yes Yes Yes Yes Yes
Fixation after live-cell staining - - - - -
Staining time > 10 min 15-45 min Expression in 8-12 hrs. > 15 min > 15 min
Ex / Em 493 / 508, 547 / 563, 643 / 663 490 / 516 ~ 644 / 665 558 / 583 560 / 580 507 / 529
Product code MT10 , MT11 , MT12 - - R237 R233



Application Products
Mitophagy Detection Mitophagy Detection Kit
Mitochondrial Phospholipid Peroxidase Detection MitoPeDPP
Mitochondrial Ferrous Ion Detection Mito-FerroGreen
Singlet oxygen specific fluorescent probe Si-DMA for Mitochondrial Singlet Oxygen Imaging
Mitochondrial Membrane Potential JC-1 MitoMP Detection Kit
Application Products
Mitochondria Staining NEW - MitoBright LT Green
NEW - MitoBright LT Red

NEW - MitoBright LT Deep Red

-Cellstain- MitoRed
-Cellstain- Rh123