Product Description of NOC Compounds
NOCs are stable NO-amine complexes that spontaneously release NO, without cofactors, under physiological conditions. The rate of NO release depends on the chemical structure of NOC. The mechanism of spontaneous NO generation by NOCs is very simple compared to other classical NO donors, such as nitroglycerin and nitropurusside, and the by-products do not interfere with cell activities. A single NOC molecule releases two NO molecules (as indicated in the reaction scheme); the release rate of the second NO molecule is very slow. NOCs can be used to add controlled amounts of pure NO to experimental systems at controlled rates with minimal side effects. The amount of NO released can be easily manipulated by altering the concentration and selection of NOC reagents. Dojindo offers four different NOCs (NOC 5, 7, 12, and 18) with different halflifes. Stock solutions of NOC prepared in alkaline solutions, such as aqueous NaOH, are relatively stable. However,the NOC stock solution should be used within one day because it degrades about 5% per day, even at -20ºC. The release of NO begins immediately after adding the stock solution to a sample solution.
Nitric Oxide Release
1. Prepare 10 mM NOC stock solution using 0.1 M NaOH. Since the NOC stock solution is not stable, keep it on an ice bath and use it in one day.
2. Add an appropriate volume of the NOC stock solution to the sample solution in which NO is to be released. To maintain the pH of the sample solution, the volume of the NOC stock solution should not exceed 1/50 of the sample volume. The sample solution should have sufficient buffering action. NO will be released immediately after the addition of the NOC stock solution.
Table 1 pH Dependency of NO Release at 37oC
2. S. Shibuta, et al., Intracerebroventricular Administration of a Nitric Oxide-releasing Compound, NOC-18, Produces Thermal Hyperalgesia in Rats. Neurosci Lett. 1995;187:103-106. (NOC 18)
3. S. Shibuta, et al., A new nitric oxide donor, NOC-18, exhibits a nociceptive effect in the rat formalin model. J Neurol Sci. 1996;141:1-5. (NOC 18)
4. N. Yamanaka, et al., Nitric Oxide Released from Zwitterionic Polyamine/NO Adducts Inhibits Cu2+-induced Low Density Lipoprotein Oxidation. FEBS Lett. 1996;398:53-56. (NOC 5, NOC 7)
5. D. Berendji, et al., Nitric Oxide Mediates Intracytoplasmic and Intranuclear Zinc Release. FEBS Lett. 1997;405:37-41.
6. T. Ohnishi, et al., The Effect of Cu2+ on Rat Pulmonary Arterial Rings. Eur J Pharmacol. 1997;319:49-55. (NOC 7)
7. Y. Adachi, et al., Renal Effect of a Nitric Oxide Donor, NOC 7, in Anethetized Rabbits. Eur J Pharmacol. 1997;324:223-226. (NOC 7)
8. Y. Minamiyama, et al., Effect of Thiol Status on Nitric Oxide Metabolism in the Circulation. Arch Biochem Biophys. 1997;341:186-192. (NOC 7)