Nitroso-PSAP is a highly sensitive water-soluble colorimetric reagent for Fe(II) detection that forms a green complex with Fe(II) (λmax=756 nm, ε=4.5×104 ). This reagent is suitable for the determination of blood serum levels of Fe, Co, Ni, and Cu. The detection range is from 5 ppb to 5 ppm under the same detection condition. Nitroso-PSAP is useful in flow injection analyses. The workable pH range of the nitroso compounds is 5.6-10.1. The molar absorptivities and maximum wavelengths of the Co, Cu, and Ni complexes are 4.0×104 (490 nm), 2.8×104 (429 nm), and 2.6×104 (394 nm), respectively.
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2. N. Ohno, et al., Spectrophotometric Determination of Iron in Boiler and Well Waters by Flow Injection Analysis Using 2-Nitroso-5-(N-propyl-N-sulphopropylamino) phenol. Analyst. 1987;112:1127-1130.
3. I. Yoshida, et al., Studies of the Stabilities of Scandium, Yttrium, and Lanthanoid-ion Complexes of 2-Nitroso-5-(N-propyl-3-sulfopropylamino)phenol. Bull Chem Soc Jpn. 1988;61:2639-2640.
4. I. Yoshida, et al., Potentiometric Studies on the Binding Properties of Protons, Some Divalent and Tervalent Metal Ions with 2-Nitroso-5-(N-propyl-3-sulfopropylamino)phenol. Anal Sci. 1988;4:69-72.
Table 1 Spectral Data of Nitroso-PSAP-Metal Complex