Nitro-TB is dissolved by hot water or hot methanol. Other organic solvents such as acetone and ether cannot solubilize Nitro-TB. Nitro-TB is easily reduced by dehydrogenase to purple formazan dye aggregates. It is used for dehydrogenase detection on agarose gels.
2. J. R. Baker, et al., Mechanism of Fructosamine Assay: Evidence against Role of Superoxide as Intermediate in Nitroblue Tetrazolium Reduction. Clin Chem. 1993;39:2460-2465.