MitoPeDPP is a cell-membrane-permeable probe, Perylene-based dye. It specifically localizes in mitochondria due to the triphenylphosphonium moiety introduced. MitoPeDPP is used to monitor lipid peroxidation in ferroptosis research.
As the excitation and emission wavelength of MitoPeDPP are 452 nm and 470 nm, respectively, the probe can be applied for lipophilic peroxide imaging in living cells.
*This probe has been developed by Dr. Shioji et al. at Fukuoka University, Department of Chemistry
Lipophilic Peroxide Detection in Mitochondria (cell line used: HepG2 cell)
A: MitoPeDPP stained Mitochondria with t-BHP treatment
B: MitoRed stained Mitochondria
C: Merged Image (A/B)
Selectivity of MitoPeDPP as a Peroxides Probe
Even though MitoPeDPP reacts with various peroxides (H2O2, t-BHP, ONOO-) in homogeneous systems (data is not shown), the MitoPeDPP is specifically-oxidized by t-BHP in mitochondria (A) but not with ROS and RNS (B).
A: MitoPeDPP stained cells with t-BHP treatment (t-BHP) and without (control).
B: MitoPeDPP stained cells with ROS or RNS exposure.
*As ROS generators, PMA (O2–・), NOC7 (NO), and SIN-1 (ONOO–) were used in the experiments.
PMA: Phorbol Myristate Acetate
SIN-1: 3-(Morpholinyl)sydnonimine, hydrochloride