How is the membrane potential detected?
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JC-1 forms aggregate (in healthy mitochondria) with red fluorescence.
As membrane potential decreases, JC-1 becomes monomers, which shows in green fluorescence. The change in ratio of red to green fluorescence is used as a indicator of mitochondrial condition. |
Easy to Use
Easy to dissolve JC-1 has been difficult to dissolve, but this kit solves the problem! |
Detect by Several Equipments Please refer to Data: Induced Apoptosis for experimental examples |
Imaging Buffer Included HEPES included Imaging Buffer keeps the cell condition optimal for a long period |
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Procedure
Data: Depolarization
HeLa cells treated with depolarizing reagent, carbonylcyanide-p-trifluoromethoxyphenylhydrazone (FCCP) were stained with JC-1 MitoMP Detection Kit. Red fluorescence indicates normal membrane potential or health mitochondria. Untreated cells showed red fluorescence, while FCCP treated cells showed little red fluorescence.
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<Experimental Condition> JC-1 concentration: 2 μmol/l in MEM, staining time: 30 min FCCP concentration: 100 μmol/l, FCCP treatment time: 1 h<Imaging Condition> Green: Ex 488 nm / Em 500-550 nm Red: Ex 561 nm / Em 560-610 nm Scale Bar: 20 μm |
Data: Induced Apoptosis
Jurkat cells treated by apoptosis inducing reagent, Staurosporine, were stained with JC-1 MitoMP Detection Kit. Procedures for these data can be found in the Technical Manual.
Fluorescence imaging of mitochondrial membrane potential in Jurkat cells
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<Imaging Condition>
Green: Ex 488 nm / Em 500-550 nm |
[Flow Cytometry]
Flow cytometric analysis of mitochondrial membrane potential in Jurkat cells |
[Plate Reader]
Fluorescence intensity ratio of mitochondrial membrane potential in Jurkat cells |
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<Detecting Condition> Green: Ex 488 nm / Em 515-545 nm Red: Ex 488 nm / Em 564-604 nm |
<Detecting Condition> Green: Ex 485 nm / Em 525-545 nm Red: Ex 535 nm / Em 585-605 nm |
Required amount of Imaging Buffer solution by vessel type
Mitophagy Induction and Detection of Mitochondrial Membrane Potential Changes
Mitochondrial condition in the carbonyl cyanide m-chlorophenyl hydrazine (CCCP) treated Parkin-expressing HeLa cells was compared with untreated cells using Mitophagy Detection Kit (MD01, MT02) and JC-1 MitoMP Detection Kit (MT09).
Result:
Mitophagy was not detected in untreated cells and the membrane potential was normal. However, reduction of membrane potential and mitophagy were observed in treated cells.
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<Experimental Condition>
Transfection of Parkin plasmid to HeLa cells Detection of Mitophagy Detection of Mitochondrial Membrane Potential |
<Detecting Condition>
[Mitophagy Detection]Ex: 561 nm, Em: 570-700 nm
[Mitochondrial Membrane Potential Detection]Green Ex: 488 nm, Em: 500-550 nm
Red Ex: 561 nm, Em: 560-610 nm
Related Categories
Cell Staining
Intracellular Fluorescent Probes
Mitochondria Research