DPPP is a non-fluorescent triphenylphosphine compound. It reacts with hydroperoxide to generate DPPP oxide that emits fluorescence at 352 nm excitation and 380 nm emission wavelengths (Fig. 1). Post-column HPLC method is used to determine phospholipid peroxide in sample solutions.
Fig 1. DPPP reaction with lipid peroxidase
When it is difficult to take out all the powder from the container,
please add the solvent into a container and dissolve it before its use.
2) K. Akasaka, et al., An Aromatic Phosphine Reagent for the HPLC-fluorescence Determination of Hydroperoxides -Determination of Phosphatidylcholine Hydroperoxides in Human Plasma. Anal Lett. 1988;21:965-975.
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5) K. Akasaka, et al., Simultaneous Determination of Hydroperoxides of Phosphatidylcholine, Cholesterol Esters and Triacylglycerols by Column-Switching High-Performance Liquid Chromatography with a Post-column Detection system. J Chromatogr. 1993;622:153-159.
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