DescriptionReferencesS.D.S
Product Description
DPPP is a non-fluorescent triphenylphosphine compound. It reacts with hydroperoxide to generate DPPP oxide that emits fluorescence at 352 nm excitation and 380 nm emission wavelengths (Fig. 1). Post-column HPLC method is used to determine phospholipid peroxide in sample solutions.
DPPP is a non-fluorescent triphenylphosphine compound. It reacts with hydroperoxide to generate DPPP oxide that emits fluorescence at 352 nm excitation and 380 nm emission wavelengths (Fig. 1). Post-column HPLC method is used to determine phospholipid peroxide in sample solutions.
Fig 1. DPPP reaction with lipid peroxidase
<Caution>
When it is difficult to take out all the powder from the container,
please add the solvent into a container and dissolve it before its use.
1) K. Akasaka, et al., Study on Aromatic Phosphines for Novel Fluorometry of Hydroperoxides(II) – the Determination of Lipid Hydroperoxides with Diphenyl-1-Pyrenylphosphine-. Anal Lett. 1987;20:797-807.
2) K. Akasaka, et al., An Aromatic Phosphine Reagent for the HPLC-fluorescence Determination of Hydroperoxides -Determination of Phosphatidylcholine Hydroperoxides in Human Plasma. Anal Lett. 1988;21:965-975.
3) K. Akasaka, et al., A Simple Fluorometry of Hydroperoxides in Oils and Foods. Biosci Biotech Biochem. 1992;56:605-607.
4) K. Akasaka, et al., High-performance Liquid Chromatography and Post-Column Derivatization with Diphenyl-1-Pyrenylphosphine for Fluorimetric Determination of Triacylglycerol Hydroperoxides. J Chromatogr. 1992;596:197-202.
5) K. Akasaka, et al., Simultaneous Determination of Hydroperoxides of Phosphatidylcholine, Cholesterol Esters and Triacylglycerols by Column-Switching High-Performance Liquid Chromatography with a Post-column Detection system. J Chromatogr. 1993;622:153-159.
6) K. Akasaka, et al., Normal-phase High-performance Liquid Chromatograohy with a Fluorimetric Postcolumn Detection System for Lipid Hydroperoxides. J Chromatogr A. 1993;628:31-35.
7) Y. Okimoto, et al., A Novel Fluoresceint Probe Diphenyl-pyrenylphosphine to Follow Lipid Peroxidation in Cell Membranes. FEBS Lett. 2000;474:137-140
2) K. Akasaka, et al., An Aromatic Phosphine Reagent for the HPLC-fluorescence Determination of Hydroperoxides -Determination of Phosphatidylcholine Hydroperoxides in Human Plasma. Anal Lett. 1988;21:965-975.
3) K. Akasaka, et al., A Simple Fluorometry of Hydroperoxides in Oils and Foods. Biosci Biotech Biochem. 1992;56:605-607.
4) K. Akasaka, et al., High-performance Liquid Chromatography and Post-Column Derivatization with Diphenyl-1-Pyrenylphosphine for Fluorimetric Determination of Triacylglycerol Hydroperoxides. J Chromatogr. 1992;596:197-202.
5) K. Akasaka, et al., Simultaneous Determination of Hydroperoxides of Phosphatidylcholine, Cholesterol Esters and Triacylglycerols by Column-Switching High-Performance Liquid Chromatography with a Post-column Detection system. J Chromatogr. 1993;622:153-159.
6) K. Akasaka, et al., Normal-phase High-performance Liquid Chromatograohy with a Fluorimetric Postcolumn Detection System for Lipid Hydroperoxides. J Chromatogr A. 1993;628:31-35.
7) Y. Okimoto, et al., A Novel Fluoresceint Probe Diphenyl-pyrenylphosphine to Follow Lipid Peroxidation in Cell Membranes. FEBS Lett. 2000;474:137-140
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