Application: Se detection colorimetric and fluorometric Nitrite detection fluorometric

ChemicalName: 2,3-Diaminonaphthalene
CAS: 771-97-1

Appearance: White or pale yellowish brown powder
MW: 158.20, C10H10N2

Storage Condition: -20ºC, protect from light
Shipping Condition: ambient temperature


Product Description
DAN is a highly selective colorimetric and fluorometric reagent for Se detection. This reagent is almost insoluble in cold water and alcohol, but it is higly soluble in water at 50ºC. DAN forms 4,5-benzopiaselenol by the complex with Se, and this complex is extracted with chloroform, toluene, or cyclohexane. Since the Se complex is volatile, Se can be determined by gas chromatography. Further, DAN generates fluorescent naphthalenetriazole by the reaction with nitrite ion, so it is also used for the fluorometric determination of nitrite.

Chemical Structure

Solvent extraction, colorimetric: Se(IV) (in toluene at pH 1.2-2.2, λmax=380 nm, ε=11,800, detection range 0-4 ppm)
Solvent extraction, fluorometric: Se(IV) (λem=520 nm in toluene, λem=538 nm in cyclohexane, detection range 1-100 ppb)

1. J. H. Wiersma, 2,3-Diaminonaphthalene as a Spectrophotometric and Fluorometric Reagent for the Determination of Nitrite Ion. Anal Lett. 1970;3:123-132.
2. G. L. Wheeler, et al., Rapid Determination of Trace Amounts of Selenium(IV), Nitrate, and Nitrate by High-pressure Liquid Chromatography Using 2,3-Diaminonaphthalene. Microchem J. 1974;19:390-405.
3. E. M. Rodriguez, et al., Critical Study of Fluorimetric Determination of Selenium in Urine. Talanta. 1994;41:2025-2031.
4. A. M. Miles, et al., Fluorometric Determination of Nitric Oxide. Methods. 1995;7:40-47.

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