SKU: P378 Categories: , ,

-Cellstain- PI Solution


Chemical Name: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide, aqueous solution
CAS: 25535-16-4(PI)

Product: 1.5 mM PI aqueous solution(1 mg PI per 1 ml water)
Appearance: Orange to red solution
MW: 668.39, C27H34I2N4

Storage Condition : -20ºC, protect from light
Shipping Condition : ambient temperature


Product Description
Propidium iodide (PI) is an ethidium bromide analog that emits red fluorescence upon intercalation with double-stranded DNA. PI does not permeate viable cell membranes, but passes through disturbed cell membranes and stains the nuclei. PI is often used in combination with a fluorescein compound, such as Calcein-AM or FDA, for simultaneous staining of viable and dead cells. The excitation and emission wavelengths of PI-DNA complex are 535 nm and 615 nm, respectively.

Chemical Structure

Staining Procedure
1.Prepare 10-50 μM PI solution with PBS or an appropriate buffer.a)
2.Add PI solution with 1/10 of the volume of cell culture medium to the cell culture.b)
3.Incubate the cell at 37oC for 10-20 min.
4.Wash cells twice with PBS or an appropriate buffer.
5.Observe the cells under a fluorescence microscope with 535 nm excitation and 615 nm emission filters.

a) Since PI may be carcinogenic, extreme care is necessary during handling. b) Or you may replac
e the culture medium with 1/10 concentration of PI buffer solution.

1. I. W. Taylor, et al., An Evaluation of DNA Fluochromes, Staining Techniques, and Analysis for Flow Cytometry. I. Unperturebed Cellpopulations. J Histochem Cytochem. 1980;28:1224-1232.
2. W. M. J. Vuist, et al., Potentiation by Interleukin 2 of Burkitt’s Lymphoma Therapy with Anti-Pan B (Anti-CD19) Monoclonal Antibodies in a Mouse Xenotransplantation Model. Cancer Res. 1989;49:3783-3788.
3. A. K. El-Naggar, et al., Single- and Double-stranded RNA Measurements by Flow Cytometry in Solid Neoplasms. Cytometry. 1991;12:330-335.
4. C. Souchier, et al., Methods for Cell Proliferation Analysis by Fluorescent Image Cytometry. Cytometry. 1995;20:203-209.
5. T. Irino, et al., Establishment of Real-Time Polymerase Chain Reaction Method for Quantitative Analysis of Asparagine Synthetase Expression. J Mol Diagn. 2004;6:217-224.

Staining Data

Fig. 2 Cell staining with PI

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