Application: Metal indicator, Ca2+ and Mg2+ detection, colorimetric

Chemical Name: 2-Hydroxy-1-(1-hydroxy-2-naphthylazo)-6-nitro-4-naphthalenesulfonic acid, sodium salt
CAS: 1787-61-7

Appearance: Black or blackish brown powder
Absorbance in borate buffer(1 mg / 100 ml, pH9): ≥0.400(around 620 nm)
MW: 461.38, C20H12N3NaO7S

Storage Condition: ambient temperature
Shipping Condition: ambient temperature


Product Description
BT in aqueous solution changes color according to pH: red at pH<6, blue at pH 7-11, and orange at pH>12. Its proton dissociation constants are reported to be pKa2=6.3 and pKa3=11.55 (m=0.08, 20ºC). BT is one of the most commonly used indicators of Ca, Mg, Zn, Cd, Pb, Hg, and rare earth metals for EDTA chelate titration. BT solution turns red in the presence of the metals at pH 10, and then changes to blue at the endpoint of the titration. Cu and Fe(III), as well as Al, Co, and Mn(III), should be masked with KCN because they interfere with the color change. Triethanolamine is also a suitable masking reagent for Al, Fe(III), and Mn(III). Since metal ions such as Mn(III) and Fe(III) oxidize BT, hydroxylamine and hydrochloride should be added to prevent oxidation.

Chemical Structure


1. P. F. Lott, et al., Spectrophotometric Determination of Thorium with Eriochrome Black T. Anal Chem. 1960;32:1702-1704.

Table 1 Metal Detection Conditions

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