Bathocuproinedisulfonic acid is a water-soluble analog of bathocuproine that emits fluorescence under UV light. Since the Bathocuproine-Cu(I) complex formed by this analog is water-soluble, Cu(I) can be determined by direct measurement of the assay solution at 483-522 nm without solvent extraction. The molar absorptivity of the bathocuproinedisulfonic acid-Cu(I) complex is 1.2×104. Dr. Faizullah developed a system to quickly determine Cu(I) at around 30 ppm by using Bathocuproine-disulfonic acid in flow injection analysis. Dr. Sawada developed a highly sensitive Cu(I) detection system using e long flow cell. Dr. Sawada’s system can detect as little as 0-15 ppb Cu(I) in water samples without pretreatment.
1. T. Ishii, et al., The Synergistic Action of the Copper Chelator Bathocuproine Sulphonate and Cysteine in Enhancing Growth of L1210 Cells in Vitro. J Cell Physiol. 1985;125:151-155.