Azomethine H is a colorimetric reagent for boron detection; it forms an orange complex with boron in aqueous solution. The molar absorptivity of this complex is 1.01×104 at 415 nm. The detection range of boron in sample solutions is 1-6 μg per ml. To detect boron in plant samples, EDTA is used to mask copper, iron, and aluminum ions. Azomethine H is used to detect microgram levels of boron in glass and steel samples.
1. Mix 9 g of Azomethine H and 2 g of ascorbic acid with 700 ml of deionized or double-distilled water.
2. Heat the mixture at 70ºC or lower to dissolve Azomethine H.
3. Add water to prepare 1,000 ml of assay solution.
4. Measure the absorbance at 410 nm to detect bron(1-6 μg / ml)
2. W. D. Basson, et al., An Automated Procedure for the Determination of Boron in Plant Tissue. Analyst. 1969;94:1135-1141.
3. R. A. Edwards, Automatic Determination of Boron(0.10-10.0 mg/l)in Raw and Waste Waters. Analyst. 1980;105:139-146.