Amine-reactive PES is a novel amino-reactive electron mediator. The mediator can able to be linked to enzymes such as glucose dehydrogenase by a covalent bond, and so is expected to be applied to next generation biosensors.
A New Generation of Electron Mediator
By modification of enzyme with Amine-reactive PES, the mediator which binds to the enzyme surface is able to transfer the electrons from reduced co-factor to the electrode. This sensing system may be useful for reducing mediator leakage from the electrode and replacement of expensive metal complex compounds.
Modification of enzymes using Amine-reactive PES is patent pending.
Electrochemical Measurement of Glucose
The concentration of glucose was measured by modifying enzyme using arPES and this sensing system has the potential to develop continuous monitoring for various target molecules such as glucose.
The Sensing System is Applicable to the Following Enzymes
– FAD-dependent glucose dehydrogenase (FAD-GDH)
– L-Lactate oxidase (Lox)
– Fructosyl amino acid oxidase (FAOx)
– Cholesterol oxidase (ChOx)
– Pyranose oxidase (PyOx)
Procedure for PES-modification
Easy procedure: Just mix enzyme and amine-reactive PES
M. Hatada, N. Loew, Y. Takahashi, J. Okuda-Shimazaki, W. Tsugawa, A. Mulchandani, K. Sode. ” Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference.” Bioelectrochemistry, 2018, 121, 185.