Mitochondria Research

The multi-functional organelle is not only involved in energy production in a cell, but other additional cellular functions. The active cycle of mitochondrial fusion and division induces morphological changes, which is called mitochondrial dynamics. Abnormalities in morphological control of mitochondria are associated with neurodegenerative diseases, metabolic disorders, aging, and so on. Therefore, the demand for long-term observation of mitochondrial dynamics has recently been increasing.



MitophagyLipophilic peroxide / Singlet oxygen / Superoxide Membrane potential / Iron ion (Fe2+) / Calcium ion (Ca2+) Mitochondria staining
Mitophagy
Reagent Mtphagy Dye Keima-Red
Principle Mtphagy Dye (included in Mitophagy Detection Kit) is a pH-sensitive fluorescent probe that accumulates in mitochondria and emits red fluorescence due to acidic conditions in a lysosome. A pH-sentitive ratiometric fluorescent protein. The excitation spectrum changes accoring to pH. This protein shows high fluorescence ratio (Ex. 550 nm/440 nm) values in a lysozome.
Fixed cell staining
Live-cell staining Yes Yes
Fixation after live-cell staining
Staining time > 30 min
Ex / Em 530 / 700 440, 550 / 620
Product code MD01MT02
Lipophilic peroxide Singlet oxygen Superoxide
Reagent MitoPeDPP Si-DMA MitoSOX
Principle A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with lipophilic peroxides in mitochondria to emit fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with singlet oxigen generated in mitochondria to emit red fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit red fluorescence.
Fixed cell staining
Live-cell staining Yes Yes Yes
Fixation after live-cell staining
Staining time > 15 min > 45 min > 10 min
Ex / Em 452 / 470 644 / 670 510 / 590
Product code M466 MT05
Membrane potential Iron ion (Fe2+) Calcium ion (Ca2+)
Reagent JC-1 MT-1 TMRM, TMRE Mito-FerroGreen Rhod2-AM
Principle A cell-permeant ratiometric fluorescent dye that accumulates in intact mitochondria due to the membrane potential. The excitation spectrum changes according to the mitochondria membrane potential. Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. MT-1 is extremely photostable and more sensitive than JC-1 and can provide equivalent detection sensitivity to TMRE.Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Diffusion of the probes occurs in a damaged mitochondria that has the decreased membrane potential. A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with ferrous ion in mitochondria to emit green fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with calcium ion in mitochondria to emit red fluorescence.
Fixed cell staining
Live-cell staining Yes YesYes Yes Yes
Fixation after live-cell staining Yes
Staining time 30-60 min 30 min30-60 min 30 min 30-60 min
Ex / Em Monomer: 514 / 529
J-aggregation: 585/590
530-560 / 570-640 550 / 575 505 / 535 553 / 576
Product code MT09 MT13M489 R002
Mitochondria staining
Reagent MitoBright LT series MitoTracker series DsRed MitoRed Rhodamine 123
Principle Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. A red fluorescent protein that binds to intact mitochondria with the use of a transfection reagent. Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential. Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential.
Fixed cell staining
Live-cell staining Yes Yes Yes Yes Yes
Fixation after live-cell staining Yes
Staining time 30 min 15-45 min Expression in 8-12 hrs. > 15 min > 15 min
Ex / Em 493 / 508, 547 / 563, 643 / 663 490 / 516 ~
644 / 665
558 / 583 560 / 580 507 / 529
Product code MT10,MT11,MT12 R237 R233
Mitochondria Research
Application Products
Mitophagy Detection Mitophagy Detection Kit
Mitochondrial Phospholipid Peroxidase DetectionMitoPeDPP
Mitochondrial Ferrous Ion Detection Mito-FerroGreen
Singlet oxygen specific fluorescent probe Si-DMA for Mitochondrial Singlet Oxygen Imaging
Mitochondrial Membrane Potential JC-1 MitoMP Detection Kit
MT-1 MitoMP Detection Kit
Mitochondria Staining MitoBright LT Green
MitoBright LT Red
MitoBright LT Deep Red
-Cellstain- MitoRed
-Cellstain- Rh123

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