Mitochondria Research
The multi-functional organelle is not only involved in energy production in a cell, but other additional cellular functions. The active cycle of mitochondrial fusion and division induces morphological changes, which is called mitochondrial dynamics. Abnormalities in morphological control of mitochondria are associated with neurodegenerative diseases, metabolic disorders, aging, and so on. Therefore, the demand for long-term observation of mitochondrial dynamics has recently been increasing.
MitophagyLipophilic peroxide / Singlet oxygen / Superoxide Membrane potential / Iron ion (Fe2+) / Calcium ion (Ca2+) Mitochondria staining
| Mitophagy | ||
|---|---|---|
| Reagent | Mtphagy Dye | Keima-Red |
| Principle | Mtphagy Dye (included in Mitophagy Detection Kit) is a pH-sensitive fluorescent probe that accumulates in mitochondria and emits red fluorescence due to acidic conditions in a lysosome. | A pH-sentitive ratiometric fluorescent protein. The excitation spectrum changes accoring to pH. This protein shows high fluorescence ratio (Ex. 550 nm/440 nm) values in a lysozome. |
| Fixed cell staining | – | – |
| Live-cell staining | Yes | Yes |
| Fixation after live-cell staining | – | – |
| Staining time | > 30 min | – |
| Ex / Em | 530 / 700 | 440, 550 / 620 |
| Product code | MD01, MT02 | – |
| Lipophilic peroxide | Singlet oxygen | Superoxide | |
|---|---|---|---|
| Reagent | MitoPeDPP | Si-DMA | MitoSOX |
| Principle | A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with lipophilic peroxides in mitochondria to emit fluorescence. | A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with singlet oxigen generated in mitochondria to emit red fluorescence. | A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit red fluorescence. |
| Fixed cell staining | – | – | – |
| Live-cell staining | Yes | Yes | Yes |
| Fixation after live-cell staining | – | – | – |
| Staining time | > 15 min | > 45 min | > 10 min |
| Ex / Em | 452 / 470 | 644 / 670 | 510 / 590 |
| Product code | M466 | MT05 | – |
| Membrane potential | Iron ion (Fe2+) | Calcium ion (Ca2+) | ||
|---|---|---|---|---|
| Reagent | JC-1 | TMRM, TMRE | Mito-FerroGreen | Rhod2-AM |
| Principle | A cell-permeant ratiometric fluorescent dye that accumulates in intact mitochondria due to the membrane potential. The excitation spectrum changes according to the mitochondria membrane potential. | Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Diffusion of the probes occurs in a damaged mitochondria that has the decreased membrane potential. | A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with ferrous ion in mitochondria to emit green fluorescence. | A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with calcium ion in mitochondria to emit red fluorescence. |
| Fixed cell staining | – | – | – | – |
| Live-cell staining | Yes | Yes | Yes | Yes |
| Fixation after live-cell staining | – | – | – | – |
| Staining time | 10-60 min | 30-60 min | 30 min | 30-60 min |
| Ex / Em | Monomer: 514 / 529 J-aggregation: 585/590 | 550 / 575 | 505 / 535 | 553 / 576 |
| Product code | MT09 | – | M489 | R002 |
| Mitochondria staining | |||||
|---|---|---|---|---|---|
| Reagent | MitoBright LT series | MitoTracker series | DsRed | MitoRed | Rhodamine 123 |
| Principle | Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. | Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. | A red fluorescent protein that binds to intact mitochondria with the use of a transfection reagent. | Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential. | Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential. |
| Fixed cell staining | – | – | – | – | – |
| Live-cell staining | Yes | Yes | Yes | Yes | Yes |
| Fixation after live-cell staining | – | – | – | – | – |
| Staining time | 30 min | 15-45 min | Expression in 8-12 hrs. | > 15 min | > 15 min |
| Ex / Em | 493 / 508, 547 / 563, 643 / 663 | 490 / 516 ~ 644 / 665 | 558 / 583 | 560 / 580 | 507 / 529 |
| Product code | MT10,MT11,MT12 | – | – | R237 | R233 |
Mitochondria Research
| Application | Products |
| Mitophagy Detection | Mitophagy Detection Kit |
| Mitochondrial Phospholipid Peroxidase Detection | MitoPeDPP |
| Mitochondrial Ferrous Ion Detection | Mito-FerroGreen |
| Singlet oxygen specific fluorescent probe | Si-DMA for Mitochondrial Singlet Oxygen Imaging |
| Mitochondrial Membrane Potential | JC-1 MitoMP Detection Kit |
| Mitochondria Staining | MitoBright LT Green MitoBright LT Red MitoBright LT Deep Red -Cellstain- MitoRed -Cellstain- Rh123 |