-SulfoBiotics- GYY4137SB06 Product Manual

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General Information

It has been recognized that hydrogen sulfi de (H2S) has an important role as a physicological active substance for vasodilation, cytoprotection, modulation of insulin secretion. Although H2S is considered as a gaseous molecule such as NO and CO, about 80% of the total sulfi de exists as hydrogen sulfi de anion (HS-) under physiological condition. In addition, H2S easily converts to various biochemical molecules such as persulfi des and polysulfi des, which react with sulfhydryl moieties in a living body. Therefore, the  precise action mechanism of H2S has not been cleared.
Sodium sulfi de (Na2S) and sodium hydrogen sulfi de (NaHS) have been widely used as a H2S donor. Only instantaneously release of H2S from Na2S and NaHS, however, is available in aqueous solution, and threse reagents give a transient stimulation of H2S.
GYY4137 is a slow-releasing hydrogen sulfi de donor developed by P. K. Moore et al.1). Since GYY4137 is water-soluble and continuously releases H2S by hydrolysis in a neutral aqueous solution, it is reported that GYY4137 shows distinct cellular effects such as anti-hypertensive, anti-atherosclerotic, and anti-tumor activities 2-5).


Fig. 1 H2S release of GYY4137 by hydrolysis

Contents

-SulfoBiotics- GYY4137 10 mg x 1

Storage Condition

Store at 0-5°C

Precaution

  • This reagent releases a toxic hydrogen sulfi de gas in a neutral aqueous solution.
  • Refer to the material safety data sheet before using the reagent.

General Protocol

  1. Dissolve 3.8 mg of GYY4137 with 0.5 ml of ddH2O to prepare 20 mmol/l GYY4137 Stock Solution.
    • Store the stock solution at -20°C and use within two months. Aliquot the solution for longer storage.
  2. Dilute the 20 mmol/l GYY4137 Stock Solution to an appropriate concentration depending on your experiment.
    • H2S release is initiated by addition of the stock solution to neutral buffers or culture medium.

Experimental Example

H2S release profi le of GYY4137 in PBS

  1. Ten microliter (10 µl) of 20 mmol/l GYY4137 Stock Solution was added to 2 ml of PBS to prepare 100 !mol/l GYY4137 (PBS) solution. 
    Then, the solution was incubated at room temperature with sealing.
  2. The yielded hydrogen sulfi de anion was quantifi ed at each time by methylene blue method.

 

H2S release from GYY4137 in PBS was slow and sustained. 


Fig. 2 H2S release profi le of 100 !mol/l GYY4137 in PBS

Reference

  1. L. Li, M. Whiteman, Y. Y. Guan, K. L. Neo, Y. Cheng, S. W. Lee, Y. Zhao, R. Baskar, C-H. Tan, and P. K. Moore, "Characterization of a Novel, Water-Soluble Hydrogen Sulfide-Releasing Molecule (GYY4137): New Insights Into the Biology of Hydrogen Sulfide", Circulation, 2008, 117, 2351.
  2. M. Whiteman, L. Li, P. Rose, C-H. Tan, D. B. Parkinson, and P. K. Moore, "The Effect of Hydrogen Sulfide Donors of Lipopolysaccharide-Induced Formation of Inflammatory Mediators in Macrophages", Antioxid. Redox Signal., 2013, 19, 1749.
  3. Z. W. Lee, J. Zhou, C-S. Chen, Y. Zhao, C-H. Tan, L. Li, P. K. Moore, and L-W. Deng, "The Slow-Releasing Hydrogen Sulfide Donor, GYY4137, Exhibits Novel Anti-Cancer Effects In Vitro and In Vivo", PLos One, 2011, 6, e21077.
  4. L. Li, B. Fox, J. Keeble, M. Salto-Tellez, P. G. Winyard, M. E. Wood, P. K. Moore, and M. Whiteman, "The complex effects of the slow-releasing hydrogen sulfide donor GYY4137 in a model of acute joint inflammation and in human cartilage cells", J. Cell. Mol. Med., 2013, 17, 365.
  5. Z. Liu, Y. Han, L. Li, G. Meng, X. Li, M. Shirhan, M. T. Peh, L. Xie, S. Zhou, X. Wang, Q. Chen, W. Dai, C-H. Tan, S. Pan, P. K. Moore and Y. Ji, "The hydrogen sulfide donor, GYY4137, exhibits anti-atherosclerotic activity in high fat fed apolipoprotein E-/- mice", Br. J. Pharmacology, 2013, 169, 1795.

SB06: -SulfoBiotics- GYY4137
Revised Apr., 05, 2024