Quantitative Determination of Target Element in Biological samples

1.Enzymatic Detection

(1) New Trinder’s Reagents (Oxidase system)

New Trinder‘s Reagents, which are highly water-soluble aniline derivatives, are applicable for the colorimetric determination of hydrogen peroxide. In the presence of a coupler such as 4-aminoantipyrine(4-AA), the Trinder‘s reagents are oxidized with peroxidase and two molecules of hydrogen peroxide and are developing the color to purple or blue.

(2) Tetrazolium Salts and Electron Mediators (Dehydrogenase system)

Tetrazolium salts have been developed to determine dehydrogenase activity which oxidizes its substrate and reduces its co-enzyme, NAD or NADP and produce NADH or NADPH. The colored formazan dyes are produced upon reduction of NADH in the presence of an electron mediator. To perform the homogenous assay in aqueous condition,  Water-Soluble Tetrazolium salts (WST) are widely applied for the diagnostic analysis and life science research due to much improved water-solubility than water insoluble tetrazolium salts, such like NBT and INT.

Detection Principle

ItemsPrinciple (Oxidase System) New Trinder’s ReagentsPrinciple (Dehydrogenase System) Tetrazolium Salts and Electron Mediators
Cholesterol
Creatine
Glucose
Lactic acid
NH3
Pyruvate
Triglycerides
Uric acid

2. Chelate Complexes Detection

(1) Other Chromogens for Clinical Chemical Assays

Dojindo offers wide range of reagents that form chelate complex with the target substance in biological sample. These reagents are applicable for quantitative determination of the target substance in the sample.

ItemsReagentPrinciple
CalciumChlorophosphonazo-IIICa2+ + Chlorophosphonazo-III → Ca2+ – Chlorophosphonazo-III complex
Copper3,5-DiBr-PAESACeluroplasmin – Cu2+ → Denaturation → Cu2+ → Reduction → Cu+  + 3,5-DiBr-PAESA → Cu+ – 3,5-DiBr-PAESA complex
IronNitroso-PSAPTransferrin – Fe3+ → Denaturation → Fe3+ → Reduction→ Fe2+  + Nitroso-PSAP → Fe2+ – Nitroso-PSAP complex
MagnesiumXB-I (Xylidyl blue-I)Mg2+  + Xylidyl blue-I → Mg2+ – Xylidyl blue-I  complex
Urinary Total Protein

PR (Pyrogallol Red)Pyrogallol Red  + Molybdate(VI) + Protein → PR – MO – Protein  complex
Zinc5-Br-PAPS


Nitro-PAPS
Physiological Ligand – Zn2+ → Denaturation → Zn2+ + 5-Br-PAPS → Zn2+ – 5-Br-PAPS complex

Zn2+  + Nitro-PAPS → Zn2+ –  Nitro-PAPS  complex

Oxidative Dyes (New Trinder’s Reagents )
Code#DescriptionCAS#
OC01ADOS82692-96-4
OC02ADPS82611-88-9
OC04ALPS82611-85-6
OC06DAOS83777-30-4
OC08HDAOS82692-88-4
OC11MAOS82692-97-5
OC13TOOS82692-93-1
OC14TOPS40567-80-4
OC21MADB209518-16-1
OC22TODB1044537-70-3
OC46EMSE

Reductive Dyes (Tetrazolium Salts )
Code#DescriptionCAS#
W201WST-1150849-52-8
W202WST-3515111-36-1
W203WST-4178925-54-7
W204WST-5178925-55-8
W217WST-9
I003INT146-68-9
M009MTT298-93-1
N011Nitro-TB298-83-9
T012TB1871-22-3

Oxidative Dyes (Others)
Code#DescriptionCAS#
K257KN-304
D006DAB7411-49-6
S302SAT-3679787-07-6
T022TMBZ54827-17-7
T036TMBZ-PS102062-46-4,
102062-36-2(free acid)

Electron Mediators
Code#DescriptionCAS#
M0031-Methoxy PMS65162-13-2
M4701-Methoxy PES133395-54-7


Chromogens for Chelate Complexes Detection
Code#DescriptionCAS#
C010Chlorophosphonazo-III1914-99-4
D0413,5-DiBr-PAESA100743-65-5
N010Nitroso-PSAP80459-15-0
X001XB-I14936-97-1
P012PR32638-88-3
B0265-Br-PAPS81608-06-2(free acid)
N031Nitro-PAPS143205-66-7(as anhydride)

Substrate
Code#DescriptionCAS#
G213Glupa-C63699-78-5
L005L-Leucyl-p-nitroanilide
HCl
16010-98-3
N055Disodium p-Nitrophenylphosphate333338-18-4(hexahydrate)

COVID-19 Announcement

Due to rising concerns regarding the COVID-19 (Coronavirus 2019) outbreak, Dojindo Molecular Technologies, Inc will be taking the necessary steps to prioritize public health and safety.

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