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Aussie Suzuki, PhD
Associate Professor
University of Wisconsin-Madison
Fluorescence microscopy has become an essential tool for biological and translational research however the diffraction limit of light limits our ability to resolve nanoscale cellular architectures. Super-resolution approaches have been developed to overcome this barrier and expansion microscopy (ExM) is among the most recent achieving nanometer- to angstrom-scale imaging through a unique strategy: physically expanding the specimen itself rather than pushing the optics. We have developed 3D-ExM an economical reproducible and user-friendly method that achieves isotropic three-dimensional expansion without environmental control or additional instrumentation. In this seminar I will describe the 3D-ExM workflow evaluate its performance and discuss how it can be extended to new biological questions.
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