Cell Counting Kit-8 (CCK-8) used for Viral Research (COVID-19 Testing)

CCK-8 is used to evaluate antiviral activity of potent compounds against virus infection. In Dr. Xia’s research work, each virus (SARS-CoV-2, MERS-CoV, HCoV-OC43, HCoV-229E and HCoV-NL63) was treated with the optimized peptide and then the mixture was added to the cells. They used the CCK-8 to assess the antiviral activity of the peptide. Also, cytotoxicity of the peptide to the cells was measured by the CCK-8.*1 The CCK-8 is a useful tool to support developing antiviral agent against virus infection.

1. S. Xia, et al., Cell Research202030, 343–355

Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion

It has been reported that the extracts of S. baicalensis have anti-viral activities. Dr. Liu et.al, determined the cytotoxicity of baicalensis extract and baicalein on Vero cells using CCK-8 in order to evaluate the antiviral efficiency of them against SARS-COV-2 virus. *2

2. H. Liu, et al., bioRxiv2020.

Scutellaria baicalensis extract and baicalein inhibit replication of SARS-CoV-2 and its 3C-like protease in vitro

Dr. Wu et al., aimed to identify Single-Domain Antibodies against SARS-CoV-2. Vero E6 cells was used in the evaluation of viral cytopathic effect (CPE)-based neutralization.CCK-8 was used to assess cell viability. *3

3. Y. Wu, et al., Cell Host Microbe.2020, doi: 10.1016/j.chom.2020.04.023.

Identification of Human Single-Domain Antibodies Against SARS-CoV-2

COVID-19 Announcement

Due to rising concerns regarding the COVID-19 (Coronavirus 2019) outbreak, Dojindo Molecular Technologies, Inc will be taking the necessary steps to prioritize public health and safety.

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