Cell Counting Kit-8 (CCK-8) used for Viral Research (COVID-19 Testing)
CCK-8 is used to evaluate antiviral activity of potent compounds against virus infection. In Dr. Xia’s research work, each virus (SARS-CoV-2, MERS-CoV, HCoV-OC43, HCoV-229E and HCoV-NL63) was treated with the optimized peptide and then the mixture was added to the cells. They used the CCK-8 to assess the antiviral activity of the peptide. Also, cytotoxicity of the peptide to the cells was measured by the CCK-8.*1 The CCK-8 is a useful tool to support developing antiviral agent against virus infection.
1. S. Xia, et al., Cell Research, 2020, 30, 343–355
It has been reported that the extracts of S. baicalensis have anti-viral activities. Dr. Liu et.al, determined the cytotoxicity of baicalensis extract and baicalein on Vero cells using CCK-8 in order to evaluate the antiviral efficiency of them against SARS-COV-2 virus. *2
2. H. Liu, et al., bioRxiv, 2020.
Dr. Wu et al., aimed to identify Single-Domain Antibodies against SARS-CoV-2. Vero E6 cells was used in the evaluation of viral cytopathic effect (CPE)-based neutralization.CCK-8 was used to assess cell viability. *3
3. Y. Wu, et al., Cell Host Microbe., 2020, doi: 10.1016/j.chom.2020.04.023.