Isothiocyanobenzyl-NTA

Chelate Labeling Reagent
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Product codeI279 Isothiocyanobenzyl-NTA
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Chemical nameN-[5-(4-IsothiOcyanatobenzyl)amido-1-carboxypentyl]iminodiacetic acid
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MWC19H23N3O7S=437.47
| Unit size | Price | FUJIFILM Wako Products code |
|---|---|---|
| 10 mg | ¥22,200 | 347-91371 |
Product Description
Isothiocyanobenzyl-NTA is utilized to modify the surfaces on which amine groups are attached. Through the NTA moiety attached on the surface, genetically expressed protein, bearing a hexahistidine extension at its terminus, can be immobilized via Ni (II) (His-Tag method). Using this technique, Dr. Noji and co-workers were able to directly abserve the rotation of F1-ATPase with a fluorescence microscope.
Chemical Structure

Manual
Technical info

Handling and storage condition
| Appearance: | White to slightly yellow powder |
|---|---|
| Purity (HPLC): | ≧ 90.0 % |
| IR spectrum: | Authentic |
| NMR spectrum: | Authentic |
| -20°C |







