CuprosGreen
Live-cell imaging of copper (I) ions
- Selective detection of intracellular monovalent copper ions
- Microscopic observation and plate reader detection possible
- Easy-to-use solution type, no need for dissolution process
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Product codeC557 CuprosGreen
| Unit size | Price | FUJIFILM Wako Products code |
|---|---|---|
| 20 μl |
Role of Copper Ions
Copper is known as a transition metal that acts as the active centre of enzymes that control vital processes such as respiration and metabolism, although it is only present in trace amounts in the body. Specifically, copper assists binds to superoxide dismutase1 (SOD1), thereby scavenging reactive oxygen species (ROS). Copper thus plays an important role in maintaining life.
In recent years, copper has also been the focus of research into cell death. While much research has been done on ferroptosis, a relatively new pathway of cell death initiated by iron (II) ions, a new pathway of cell death initiated by copper (I) ions, distinct from ferroptosis (cuproptosis), has been discovered1). CuprosGreen is a fluorescent probe that selectively reacts with intracellular copper (I) ions and emits fluorescence. Its membrane permeability allows live-cell imaging of copper (I) ions.
1) Tsvetkov et al., Science, 2022, 375, 1254–1261
For imaging with fluorescence microscopy and quantifying with a plate reader
In HeLa cells, we evaluated changes in intracellular CuCl2 addition (which is reduced intracellularly to monovalent copper ions) and found that CuCl2 addition increased intracellular monovalent copper ion levels compared to control by both confocal microscopy and plate reader detection methods.
Manual
Q & A
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Q
Why is it necessary to use serum-free medium for the working solution?
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A
Preparation of CuprosGreen in serum containing medium at the time of staining will result in lower fluorescence intensity; for optimal performance of CuprosGreen we recommend preparation in serum free medium.
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Q
Is it possible to prepare the working solution in PBS or HBSS?
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A
The incubation time for CuprosGreen is 3 hours and it is recommended that it is prepared in serum-free medium to reduce cytotoxicity caused by prolonged incubation.
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Q
Why does the protocol state that cells should be observed after staining without washing?
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A
CuprosGreen dye diffuses during washing.
To optimise the performance of CuprosGreen, it is recommended to observe without washing.
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Q
Are there any precautions with the dilution of CuprosGreen DMSO solution?
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A
If CuprosGreen DMSO solution is added to serum-free medium, CuprosGreen may precipitate in the medium. Ensure that the working solution is prepared by adding additional DMSO and serum-free medium to the CuprosGreen DMSO solution.
Handling and storage condition
| Appearance： | Colorless to slightly brown liquid |
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| Dye content： | To pass test |
| Store at -20 °C, protect from light |
