Targets in Cell Proliferation, Viability, and Cytotoxicity
Various assays are used for assessing cell proliferation, viability, and cytotoxicity. Examples include optical counting, [3H] thymidine, MTT, WST, lactate dehydrogenase (LDH) assay, and more. This selection guide aims to provide you with information that will allow you to pick the method most suitable for your specific experimental needs.
Proliferation
Measure cell count and/or DNA content to reveal cell division and DNA replication, respectively Methods: - Optical counting with hemocytometer - DNA quantification using fluorophores
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Viability
Measure parameters of cellular health to determine the intracellular impact of cytotoxicity Methods: -Measuring evidence of cellular metabolic activity such as esterase activity, ATP, NADH dehydrogenase activity via MTT or WST, and so on. |
Cytotoxicity
Measure indicators of cell death to evaluate cytotoxicity Methods: -Measure the activity of lactate dehydrogenase leaked out of damaged cell membranes -Use membrane-impermeable dyes to selectively stain inside cells with damaged membranes |
Learn more about cell analysis
(“Reagent Selection Guide, Cell Proliferation / Cell Viability / Cytotoxicity ”)
Chapters:
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