Mitochondria Research for Mammalian Cells

∼ Features ∼

  • Beginner Friendly
    • Easy to Dissolve
    • Imaging Buffer optimize Cell Condition for Measurements

 



Mitochondria synthesize ATP using oxygen to produce necessary energy for living cells. Lowering of mitochondrial activity and dysfunction are known to be closely related to cancer, aging, and neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases. Mitochondrial membrane potential is a parameter used to measure with mitochondrial condition.


§ How is the membrane potential detected?

JC-1 forms aggregate (in healthy mitochondria) with red fluorescence.

As membrane potential decreases, JC-1 becomes monomers, which shows in green fluorescence.

The change in ratio of red to green fluorescence is used as a indicator of mitochondrial condition.

§ Easy to Use


Easy to dissolve

JC-1 has been difficult to dissolve, but this kit solves the problem!

Detect by Several Equipments

Please refer to Data: Induced Apoptosis for experimental examples

Imaging Buffer Included

HEPES included Imaging Buffer keeps the cell condition optimal for a long period

§ Procedure


§ Data: Depolarization

HeLa cells treated with depolarizing reagent, carbonylcyanide-p-trifluoromethoxyphenylhydrazone (FCCP) were stained with JC-1 MitoMP Detection Kit. Red fluorescence indicates normal membrane potential or health mitochondria. Untreated cells showed red fluorescence, while FCCP treated cells showed little red fluorescence.

<Experimental Condition>

JC-1 concentration:
2 μmol/l in MEM, staining time: 30 min
FCCP concentration:
100 μmol/l, FCCP treatment time: 1 h

<Imaging Condition>

Green: Ex 488 nm / Em 500-550 nm
Red: Ex 561 nm / Em 560-610 nm
Scale Bar: 20 μm

§ Data: Induced Apoptosis

Jurkat cells treated by apoptosis inducing reagent, Staurosporine, were stained with JC-1 MitoMP Detection Kit. Procedures for these data can be found in the Technical Manual.


[Fluorescence Microscope]

Fluorescence imaging of mitochondrial membrane potential in Jurkat cells

<Imaging Condition>

Green: Ex 488 nm / Em 500-550 nm
Red: Ex 561 nm / Em 560-610 nm
Scale Bar: 80 μm



[Flow Cytometry]

Flow cytometric analysis of mitochondrial membrane potential in Jurkat cells

[Plate Reader]

Fluorescence intensity ratio of mitochondrial membrane potential in Jurkat cells

<Detecting Condition>

Green: Ex 488 nm / Em 515-545 nm
Red: Ex 488 nm / Em 564-604 nm
<Detecting Condition>

Green: Ex 485 nm / Em 525-545 nm
Red: Ex 535 nm / Em 585-605 nm

§ The Number of Usable Assays



Storage Condition: 0-5 oC and protect from light

Shipping Condition: ambient temperature


Item # Description/Size Availability Qty Break Price Quantity
MT09-10
1 set
1-2 business days 1 $230.00

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For Research Use Only Products