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Exosomes are a form of extracellular vesicle (EV), which may contribute to malignant transformation and the metastasis of cancer. Consequently, intercellular communication via exosomes is attracting considerable interest in the scientific community. To shed light on such communication, labeling techniques based on fluorescent dyes have been used. Fluorescent dyes that label the cellular membrane are commonly used for exosome labeling because the lipid bilayer in exosomes is a suitable labeling target. ExoSparkler series can be used for staining of purified exosomal membrane or protein, which allows imaging of labeled exosomes taken up by cells.
Our ExoSparkler Exosome Protein Labeling Kits provide everything from fluorescence labeling to purification
ExoSparkler series contains filtration tubes available for the removal of dyes unreacted after fluorescence labeling, as well as an optimized protocol for labeling exosomes. Our ExoSparkler series makes it possible to prepare fluorescence labeling of exosomes using the simple procedure.
ExoSparkler series product comparison
Exosomes were purified by ultracentrifugation (10μg exosome protein) and stained with each dye. Labeled exosomes were added to HeLa cells (1.25×104 cells), and the cells were incubated for 24 hours. Cells were washed, and immunofluorescence images showing labeled exosomes were observed.
Green: Ex 488nm/Em 490-540nm
Red: Ex 561nm/Em 570-640nm
Deep Red: Ex 640nm/Em 640-760nm
Localization of exosomes
Exosomes stained with ExoSparkler Exosome Protein Labeling Kit-Deep Red（Item#: EX06, Protein Dye-Deep Red）or ExoSparkler Exosome Membrane Labeling Kit-Red（item#：EX02, Mem Dye-Red）were added to HeLa cells, and the cells were co-stained with lysosome staining reagent (Green).
Exosomes were purified by ultracentrifugation (5μg exosome protein) and stained with each dye. Labeled exosomes were added to HeLa cells (0.75×104 cells), and the cells were incubated for 3.5 hours. After that, cells were stained with lysosome staing reagent (Green). Cells were washed, and immunofluorescence images showing labeled exosomes were observed.
Protein Dye-Deep Red (purple) : Ex 640nm/Em 640-760nm
MemDye-Red (Red) : Ex 561nm/Em 570-640nm
Lysosome staing reagent (Green). : Ex 488nm/Em 490-540nm