04 Intracellular Fluorescent Probes

Calcium Kit II - Fluo 4

Non wash - Intracellular Calcium assay Kit

Complete kit with all reagents needed for intracellular Ca²⁺ measurements.
Ca-probes are pre-divided into individual tubes [10 plates/kit].
Include probe solubilizer and leakage inhibitor

Product code

CS32　 Calcium Kit II - Fluo 4

Unit size	Price	Item Code
10 plates	Find your distributors	CS32-10

Component

10 plates	・Fluo 4-AM ・Dimethylsulfoxide ・5% Pluronic F-127 ・5% Cremophor El ・250 mmol/l Probenecid ・Hanks' HEPES Buffer (10X) ・Quenching Buffer	50 μg x10 2 mlx1 2.5 ml x1 2.5 ml x1 1.3 ml x1 6 ml x1 55 ml x1

C of A

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Description

The Calcium Kit II - Fluo 4 is a screening kit containing Fluo 4-AM, a reagent for the measurement of intracellular Ca2+, as well as buffers and other components necessary for the measurement.
Depending on the cell type and drugs to be added, the concentrations of Pluronic F-127 or Cremophor EL (solubilizing agents for Fluo 4-AM) and Probenecid (an inhibitor of anion transporters) can be adjusted as required.The kit includes a reagent that eliminates the need for conventional cell washing procedures by quenching the fluorescence of Fluo 4 present in the extracellular space. This allows for the rapid processing of a large number of samples, making the kit suitable for high-throughput screening (HTS).
However, depending on the cell type and added drugs, variations such as "high or low response" or "interactions with ligands" may occur. For measurements requiring minimal impact on the measurement system, we recommend using the sister product, "Calcium Kit - Fluo 4" (Wash Type).
The prepared Loading Buffer allows for the measurement of ten plates, each with 96 wells. For measurement, use a clear-bottom plate and a plate reader capable of downward excitation and fluorescence measurement.

Manual

Technical info

1) Complete kit with all reagents needed for intracellular Ca2+ measurements.
2) Ca-probes are pre-divided into individual includes [10 plates/kit].
3) Concentrations of probe solubilizer and leakage inhibitor can be adjusted as required.
4) Non-Wash type that does not require washing.
*Please refer to the protocol for usage instructions.

Principle

Q & A

Q Are there any differences in the measurement results between Wash and Non-Wash?: A

There is almost no difference.
However, since the non-wash type uses a quenching buffer, the rate of change in fluorescence intensity or response may differ depending on the cells or stimulants used.

Q This is my first time performing this measurement. Could you please explain the principle, measurement method, and troubleshooting methods for Ca²⁺ probes?

A Practical Guide to Measuring Intracellular Ca²⁺ for Beginners

-Insight from Customer Support-

We have created a practical guide to intracellular Ca²⁺ measurement for beginners.
It includes information on the principles and characteristics of Ca²⁺ probes, measurement methods, and troubleshooting techniques. Please refer to it for guidance.
The protocol is available for download via the link below.

A Practical Guide to Measuring Intracellular Ca²+ for Beginners

Handling and storage condition

Handling and storage condition
-20°C,Protect from light
Danger / harmful symbol mark

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Calcium Kit II - Fluo 4

Calcium Kit II - Fluo 4