Calcium Kit - Fluo 4
Intracellular Calcium Ion Measurement Kit
- Complete kit with all reagents needed for intracellular Ca2+ measurements.
- Ca-probes are pre-divided into individual tubes [10 plates/kit].
- Include probe solubilizer and leakage inhibitor
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Product codeCS22 Calcium Kit - Fluo 4
| Unit size | Price | Item Code |
|---|---|---|
| 10 plates | Find your distributors | CS22-10 |
| 10 plates | ・Fluo 4-AM ・Dimethylsulfoxide ・5% Pluronic F-127 ・5% Cremophor El ・250 mmol/l Probenecid ・Recording Medium(2X) |
50 μg x10 2 ml x1 2.5 ml x1 2.5 ml x1 1.3 ml x1 100 ml x1 |
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Description
The Calcium Kit - Fluo 4 is a screening kit containing Fluo 4-AM, a reagent for the measurement of intracellular Ca2+, as well as buffers and other components necessary for the measurement.
Depending on the cell type and drugs to be added, the concentrations of Pluronic F-127 or Cremophor EL (solubilizing agents for Fluo 4-AM) and Probenecid (an inhibitor of anion transporters) can be adjusted as required.
Various fluorescence plate readers and injectors used for high throughput screening allow real-time measurement of changes in intracellular calcium ion concentration caused by drug addition.
Technical info
1) Complete kit with all reagents needed for intracellular Ca2+ measurements.
2) Ca-probes are pre-divided into individual includes [10 plates/kit].
3) Concentrations of probe solubilizer and leakage inhibitor can be adjusted as required.
4) Washing method with minimal impact on the measurement.
*Please refer to the protocol for usage instructions.
Principle
Q & A
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Q
Are there any differences in the measurement results between Wash and Non-Wash?
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A
There is almost no difference.
However, since the non-wash type uses a quenching buffer, the rate of change in fluorescence intensity or response may differ depending on the cells or stimulants used.
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Q
This is my first time performing this measurement. Could you please explain the principle, measurement method, and troubleshooting methods for Ca²⁺ probes?
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A
A Practical Guide to Measuring Intracellular Ca²+ for Beginners
-Insight from Customer Support-
We have created a practical guide to intracellular Ca²⁺ measurement for beginners.
It includes information on the principles and characteristics of Ca²⁺ probes, measurement methods, and troubleshooting techniques. Please refer to it for guidance.
The protocol is available for download via the link below.A Practical Guide to Measuring Intracellular Ca²+ for Beginners
Handling and storage condition
| -20°C,Protect from light | |
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Danger / harmful symbol mark |
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