Cellular function analysis

Dojindo offers various assay kits and reagents to evaluate the key indicators of Cell Function Analysis.
Information on more than 100 products are available in Analytical Reagents for Cellular Function
We hope it would be helpful to advance your research on Cell Function Analysis.

What is Cellular function analysis?

Cellular function analysis has become an important research area in medical field, including elucidation of disease mechanisms and development of the pharmaceuticals. It has also become a focus for cosmetics industry.

Especially, the intracellular metabolism, cellular senescence, and the mitochondrial function are known to be closely related to each other. Thus, it is recommended to use the multiple indicators for the analysis.

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Intracellular Metabolism

Understanding the intracellular metabolism, such as glycolysis, the TCA cycle and mitochondrial electron transport, is essential for analyzing the status of the cell.

We offer the ready-to-use kits and reagents to analyze the intracellular metabolism with various indicators evaluating the metabolites and nutrients uptake, including glucose, lactate, NAD(P)/NAD(P)H, and glutamine/cystine, as well as the mitochondrial function, including mitochondrial membrane potential, and OCR (Oxygen Consumption Rate).

Related product
Glycolysis/OXPHOS Assay Glycolysis/OXPHOS Assay Kit G270
Glycolysis/Mitochondrial Membrane Potential Assay Glycolysis/JC-1 MitoMP Assay Kit G272
ATP Measurement ATP Assay Kit-Luminescence A550
ADP/ATP Ratio Assay ADP/ATP Ratio Assay Kit-Luminescence A552
Glucose Metabolism Assay Glucose Assay Kit-WST G264
Glutamine Assay Glutamine Assay Kit-WST G268
Glutamate Assay Glutamate Assay Kit-WST G269
α-Ketoglutaric Acid Measurement α-Ketoglutarate Assay Kit-Fluorometric K261
Lactic Acid Measurement Lactate Assay Kit-WST L256
NAD/NADH Assay NAD/NADH Assay Kit-WST N509
NADP/NADPH Assay NADP/NADPH Assay Kit-WST N510
Glucose uptake capacity assay Glucose Uptake Assay Kit-Blue UP01
Glucose uptake capacity assay Glucose Uptake Assay Kit-Green UP02
Glucose uptake capacity assay Glucose Uptake Assay Kit-Red UP03
Amino Acid Uptake Assay Amino Acid Uptake Assay Kit UP04
Cystine Uptake Assay Cystine Uptake Assay Kit UP05
Fatty Acid Uptake Assay Fatty Acid Uptake Assay Kit UP07

Mitochondria

Dojindo offers a broad range of reagents and kits for the research on mitochondria, including Mitophagy Detection, Oxygen Consumption Rate Assay, ROS Assay/Detection Kit, as well as the Assay/Detection of lipid peroxidation caused by the ROS.

Mitochondrial membrane potential (MtMP) dependent fluorescent probes for mitochondria staining are also available.

Related product
OCR Plate Assay Kit Extracellular OCR Plate Assay Kit E297
Glucose Metabolism Assay Glucose Assay Kit-WST G264
Lactic Acid Measurement Lactate Assay Kit-WST L256
Mitochondrial Membrane Potential Detection JC-1 MitoMP Detection Kit MT09
Mitochondria Research MT-1 MitoMP Detection Kit MT13
Mitophagy Detection Mitophagy Detection Kit MD01
Mitophagy Detection Mtphagy Dye MT02
Mitochondrial Staining MitoBright LT Green MT10
Mitochondrial Staining MitoBright LT Red MT11
Mitochondrial Staining MitoBright LT Deep Red MT12
Mitochondrial staining MitoBright IM Red for Immunostaining MT15
Mitochondrial Superoxide Detection MitoBright ROS Deep Red - Mitochondrial Superoxide Detection MT16
Mitochondrial Iron Detection Mito-FerroGreen M489
Mitochondrial Singlet Oxygen Imaging Si-DMA for Mitochondrial Singlet Oxygen Imaging MT05
Mitochondrial Lipid Peroxide Detection MitoPeDPP M466

Cellular Senescence

Understanding the apoptosis, necrosis, autophagy and cellular senescence is critical for Cell Function Analysis. Especially, the cellular senescence has been attracting the attention in various fields since the report of the SASP (senescence-associated secretory phenotype), and the stem cell senescence.

We offer four types of kits and reagents with different indicators and methods.

■ Increased expression of SA-β-Gal

■ Accumulation of DNA damage

■ Nucleolar hypertrophy
Related product
Senescence Cell Detection Cellular Senescence Detection Kit - SPiDER-βGal SG03
Senescence Cell Detection （for Microplate） Cellular Senescence Plate Assay Kit - SPiDER-βGal SG05

Experiments using multiple indicators

■ Example: Observing the intracellular metabolic changes associated with cellular senescence

In cellular senescence induced cells, an increased expression of SA-β-gal and the irreversible cell-cycle arrest are observed, and those cells with accumulation of DNA damage shift the energy metabolism to glycolysis as the consequence of the decline in mitochondrial function.

The changes in cell function were evaluated with doxorubicin (DOX) induced senescent A549 cells by conducting the experiments using multiple indicators.

Related products

Cell Proliferation/ Cytotoxicity Assay
Cell Proliferation / Cytotoxicity Assay, CCK-8, WST-8 Cell Counting Kit-8 CK04
Cytotoxicity Assay Cytotoxicity LDH Assay Kit-WST CK12
Cell Proliferation / Cytotoxicity Assay Kit Viability/Cytotoxicity Multiplex Assay Kit CK17
Cell Count Normalization Cell Count Normalization Kit C544
Autophagy
Autophagy (Autolysosome) Detection DALGreen - Autophagy Detection D675
Autophagy (Autophagosome) Detection DAPGreen - Autophagy Detection D676
Autophagy (Autophagosome) Detection DAPRed - Autophagy Detection D677
Oxidative Stress
ROS Detection ROS Assay Kit -Highly Sensitive DCFH-DA- R252
Photo-oxidation Resistant ROS Assay Kit ROS Assay Kit -Photo-oxidation Resistant DCFH-DA- R253
Mitochondrial Superoxide Detection MitoBright ROS Deep Red - Mitochondrial Superoxide Detection MT16
Mitochondrial Singlet Oxygen Imaging Si-DMA for Mitochondrial Singlet Oxygen Imaging MT05
Lipid Peroxide Detection Liperfluo L248
Lipid Peroxidation Assay Lipid Peroxidation Probe -BDP 581/591 C11- L267
Mitochondrial Lipid Peroxide Detection MitoPeDPP M466
Intracellular Iron Ion Measurement FerroOrange F374
Mitochondrial Iron Detection Mito-FerroGreen M489
Glutathione Quantification GSSG/GSH Quantification Kit G257
Lysosome
Lysosomal Acidic pH Detection Kit Lysosomal Acidic pH Detection Kit L266
Lysosome staining reagent (Deep Red) LysoPrime Deep Red - High Specificity and pH Resistance L264
Lysosomal Acidic pH Detection pHLys Red - Lysosomal Acidic pH Detection L265
Endocytosis
Endocytosis Detection ECGreen-Endocytosis Detection E296
Other Organelles
Cell Membrane Staining PlasMem Bright Green P504
Cell Membrane Staining PlasMem Bright Red P505
Nucleolus Fluorescent Staining Nucleolus Bright Green N511
Nucleolus Fluorescent Staining Nucleolus Bright Red N512
Exosome
Exosome Membrane Fluorescent Staining ExoSparkler Exosome Membrane Labeling Kit-Green EX01
Exosome Membrane Fluorescent Staining ExoSparkler Exosome Membrane Labeling Kit-Red EX02
Exosome Membrane Fluorescent Staining ExoSparkler Exosome Membrane Labeling Kit-Deep Red EX03
Exosome Protein Fluorescent Staining ExoSparkler Exosome Protein Labeling Kit-Green EX04
Exosome Protein Fluorescent Staining ExoSparkler Exosome Protein Labeling Kit-Red EX05
Exosome Protein Fluorescent Staining ExoSparkler Exosome Protein Labeling Kit-Deep Red EX06
Exosome Isolation ExoIsolator Exosome Isolation Kit EX10
Filter for exosome isolation kit ExoIsolator Isolation Filter EX11
Lipid Droplet
Lipid Droplet Staining Lipi-Blue LD01
Lipid Droplet Staining Lipi-Green LD02
Lipid Droplet Staining Lipi-Red LD03
Lipid Droplet Staining Lipi-Deep Red LD04
Lipid Droplet Assay Lipid Droplet Assay Kit - Blue LD05
Lipid Droplet Assay Lipid Droplet Assay Kit - Deep Red LD06

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Dojindo offers various assay kits and reagents to evaluate the key indicators of Cell Function Analysis.

Information on more than 100 products are available in Analytical Reagents for Cellular Function.
We hope it would be helpful to advance your research on Cell Function Analysis.

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