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Cell Counting Kit-8
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December 2016 Publications~

RF-EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells

L Su, X Wei, Z Xu, G Chen - Bioelectromagnetics, 2016

... Cell Viability Analysis. Cell viability was determined using Cell Counting Kit-8
(CCK-8, Dojindo Molecular Technologies, Kumamoto, Japan). After 24 h of exposure
to RF-EMF, cells were re-seeded into 96-well plates at 5000 ...

MicroRNA-98 Suppress Warburg Effect by Targeting HK2 in Colon Cancer Cells

W Zhu, Y Huang, Q Pan, P Xiang, N Xie, H Yu - Digestive Diseases and Sciences, 2016

... Cell Proliferation Assay Indicated cells after oligonucleotide transfection were seeded in 96-well
plates at a concentration of 2000 cells per well after 24 h. Cell proliferation assay was performed
using Cell Counting Kit-8 (Dojindo) at 24, 48, 72, and 96 h, respectively. ...

[HTML] Matrine induces Akt/mTOR signalling inhibition-mediated autophagy and apoptosis in acute myeloid leukaemia cells

J Wu, G Hu, Y Dong, R Ma, Z Yu, S Jiang, Y Han, K Yu… - Journal of Cellular and …, 2016

... FMK (Selleck Chemicals). After 12, 24 or 48 hrs, cell viability was measured using
the Cell Counting Kit-8 (CCK-8) assay (Dojindo, Kumamoto, Japan) according to
the manufacturer's instruction. The absorbance was measured ...

Simulated microgravity affects some biological characteristics of Lactobacillus acidophilus

D Shao, L Yao, J Zhu, J Shi, M Jin, Q Huang, H Yang - Applied Microbiology and …, 2016

... The treatment was carried out for 48 h at 37 °C in a 5% CO2 incubator. Subsequently, a 20 µL
volume of CCK-8 assay reagent (Cell Counting Kit-8, Dojindo, Japan) was added to a 200 µL
cell suspension per well in the 96-well plate in triplicate for each treatment. ...

PEPTIDES HAVING OSTEOBLAST GROWTH-PROMOTING ACTIVITY AND USE THEREOF

C Yamazaki, S Harada, K Watabe, M Kim - US Patent 20,160,362,466, 2016

... As a control, medium without a synthetic peptide was added. The reaction solution (10 µg/well)
of Cell Counting Kit-8 (Dojindo Laboratories) was added to each well. The plate was incubated
for 3 hours, and the absorbance at 450 nm was measured with a microplate reader. ...

Physicochemical, antioxidant and cytotoxic properties of Xao tam phan (Paramignya trimera) root extract and its fractions

N Van Tang, JA Sakoff, CJ Scarlett - Chemistry & Biodiversity, 2016

... Keratinocyte Serum-Free Medium (KSFM) were purchased from Gibco by Life
Technologies (Grand Island, NY, USA). Dojindo Cell Counting Kit-8 (CCK-8) was
obtained from Dojindo Molecular Technologies (INC., Maryland, USA). ...

[PDF] Metalloporphyrin-encapsulated Biodegradable Nanosystems for Highly Efficient MRI-Guided Sonodynamic Cancer Therapy

P Huang, X Qian, Y Chen, L Yu, H Lin, L Wang, Y Zhu… - Journal of the American …, 2016

... Cell Counting Kit-8 (CCK-8) and phosphate buffer solution (PBS) were ob- tained from Shanghai
Ruicheng Bio-Tech Co., LTD. ... N,N- bis(carboxymethyl)aminomethyl]fluorescein and tetraacetoxyme-
thyl ester (Calcein-AM) were purchased from Dojindo Molecular Technologies ...

Graphene oxide-chitosan nanocomposites for intracellular delivery of immunostimulatory CpG oligodeoxynucleotides

H Zhang, T Yan, S Xu, S Feng, D Huang, M Fujita… - Materials Science and …, 2017

... The mouse leukemic monocyte macrophage RAW264.7 cell line was purchased from Cell Bank
of Chinese Academy of Sciences (Shanghai, China). Cell Counting Kit-8 (CCK-8) was purchased
from Dojindo (Kumamoto, Japan). 2.2. Preparation of GO-CS nanocomposites. ...

[PDF] Ursolic acid protects MC3T3-E1 cells against dexamethasone-mediated apoptosis, ROS generation and inflammation through activation of IGF-1

J Chen, D He, Q Li, Z Wu, W Huang, Y Zhu - Int J Clin Exp Med, 2016

... Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8, Dojindo, Kumamoto, Japan) assay
as described previously [19]. In brief, MC3T3-E1 cells were seeded in 96-well plates at density
of 3×103 cells/well and cultured in a humidified atmosphere at 37°C with 5% CO2. ...

Regulatory factor X1 depresses ApoE-dependent Aß uptake by miRNA-124 in microglial response to oxidative stress

CZ Feng, JB Yin, JJ Yang, L Cao - Neuroscience, 2016

... Cell cytotoxicity assay. The cell cytotoxicity assay was performed using Cell Counting Kit-8
(CCK-8) (Dojindo Molecular Technologies) according to the manufacturer's instructions.
Approximate 0.25 × 10 5 cells were plated in 24-well plates for overnight. ...

[HTML] Effects of resveratrol on ARPE-19 cell proliferation and migration via regulating the expression of proliferating cell nuclear antigen, P21, P27 and p38MAPK/MMP-9

XN Hao, WJ Wang, J Chen, Q Zhou, YX Qu, XY Liu… - International Journal of …, 2016

... Res and dimethyl sulfoxide (DMSO) were bought from Sigma (USA); cell counting kit-8
(CCK-8) was from Dojindo (Japan); mouse anti-human PCNA, rabbit anti-human P21 were from
Proteintech (USA); rabbit anti-human P27 was from Abcam (UK); rabbit anti-human MMP-9 ...

[HTML] The 3D mechanical environment and chemical milieu influence the hMSC fibrogenesis and fibroblast-to-myofibroblast transition

R Xu, F Besenbacher, M Chen - RSC Advances, 2017

... Life and Analytical Sciences, Shelton, CT). 2.5. Cell viability/proliferation study. Cell
viability/proliferation was determined by Cell Counting Kit-8 (CCK-8, Dojindo,
Kumamoto, Japan) analysis. After culturing cells in conditions ...

[HTML] Neuropeptide Y1 Receptor Regulates Glucocorticoid-Induced Inhibition of Osteoblast Differentiation in Murine MC3T3-E1 Cells via ERK Signaling

W Yu, C Zhu, W Xu, L Jiang, S Jiang - International Journal of Molecular Sciences, 2016

... Cell proliferation and apoptosis were assessed by cell counting kit-8 (CCK-8) assay and
cleaved caspase expression, respectively. Osteoblast differentiation was evaluated by
Alizarin Red S staining and osteogenic marker gene expressions. ...

SIRT4 overexpression protects against diabetic nephropathy by inhibiting podocyte apoptosis

JXIA SHI, Q WANG, H Li, Q Huang - system, 2017

... Cell prolif- eration was then determined using a Cell Counting kit (CCK)-8 assay (Dojindo
Laboratories, Kumamoto, Japan) according to the manufacturer's protocol. ... (A) Proliferation was
analyzed by Cell Counting kit-8 assay after 0, 12, 24, 36, 48, 60 and 72 h glucose ...

[HTML] isoorientin induces apoptosis, decreases invasiveness, and downregulates VegF secretion by activating aMPK signaling in pancreatic cancer cells

T Ye, J Su, C Huang, D Yu, S Dai, X Huang, B Chen… - OncoTargets and therapy, 2016

... After 24 hours, the cells were washed with PBS once, the medium containing isoorientin
was discarded, and 100 µL of FBS-free medium with 10 µL of the Cell Counting Kit
8 (CCK8; Dojindo, Kumamoto, Japan) reagent were added. ...

A Targeted and Stable Polymeric Nanoformulation Enhances Systemic Delivery of mRNA to Tumors

Q Chen, R Qi, X Chen, X Yang, S Wu, H Xiao, W Dong - Molecular Therapy, 2017

Page 1. Original Article A Targeted and Stable Polymeric Nanoformulation
Enhances Systemic Delivery of mRNA to Tumors ...

[HTML] Manipulation of Schwann cell migration across the astrocyte boundary by polysialyltransferase-loaded superparamagnetic nanoparticles under magnetic field

B Xia, L Huang, L Zhu, Z Liu, T Ma, S Zhu, J Huang… - International Journal of …, 2016

... CCK-8 assays. Cell viability and cytotoxicity were analyzed using the Cell Counting
Kit 8 (CCK-8; Dojindo, Kumamoto, Japan) according to the manufacturer's instructions.
Briefly, different concentrations of PEI-SPIONs (0, 1, 2 ...

[PDF] Inhibitor of Nicotinamide Phosphoribosyltransferase Sensitizes Glioblastoma Cells to Temozolomide via Activating ROS/JNK Signaling Pathway

J Feng, PF Yan, H Zhao, FC Zhang, WH Zhao, M Feng - BioMed Research …, 2016

... against tubulin were purchased from Abcam (Cambridge, UK). The non-radioactive cell 98 viability
assay (Cell Counting Kit-8) was purchased from Dojindo Molecular Technologies, 99 Inc.
(Kumamoto, Japan). Terminal deoxynucleotidyl transferase dUTP nick end labeling 100 ...

5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells

HG Kang, S Kim, KH Lee, S Jin, SH Kim, K Lee, H Jeon… - Small, 2016

... Cytotoxicity Assay: Cell viability was assessed using the colorimetric cell counting
kit-8 (CCK-8) (Dojindo Laboratories, Kyoto, Japan). Cells were plated onto 24-well
plates at 4 × 10 5 cells in 10% FBS-MEM (500 µL) and cultured ...

Cortical Actin Alteration at the Matrix-Side Cytoplasm in Lung Adenocarcinoma Cells and Its Significance in Invasion

S Tomii, T Akashi, N Ando, T Tamura, A Sakurai… - Pathobiology, 2016

... for 15 h, and then incubated with DMEM/FCS containing blebbistatin for 48 h. Next, the medium
was replaced with 100 µL of DMEM/FCS containing 10 µL of Cell Counting Kit-8 (Dojindo, Osaka,
Japan), incubated for 30 min, and the optical density at 450 nm was measured. ...

COMBINED EFFECTS OF SILICATE, CALCIUM AND MAGNSIUM IONS ON OSTEOBLAST-LIKE CELL FUNCTIONS

A Obata, T Ogasawara, S Yamada, T Kasuga - Advances in Ceramic Armor, …, 2016

[HTML] Zedoarondiol Inhibits Platelet-Derived Growth Factor-Induced Vascular Smooth Muscle Cells Proliferation via Regulating AMP-Activated Protein Kinase Signaling …

H Mao, T Tao, D Song, M Liu, X Wang, X Liu, D Shi - Cellular Physiology and …, 2016

... (Hangzhou, Zhejiang, China). The Cell Counting Kit-8 (CCK-8) kit was purchased from Dojindo
Laboratories (Kumamoto, Japan). The Bromodeoxyuridine (BrdU) Cell Proliferation ELISA
Kit was obtained from Roche Diagnostics (Mannheim, Germany). ...

[PDF] Tripterygium wilfordii polyglycoside reduces the proliferation and inflammatory cytokines secretion of Hacat cells by regulating the balance of neutrophil elastase and …

N Chen, J Sun, Y Song, J Ge, Y Shi, L Zhang - Int J Clin Exp Pathol, 2016

... (Massachusetts, USA). Penicillin-streptomycin, phosphate buffer saline (PBS) buffer and Cell
Counting Kit-8 (CCK-8, No. CK04) were from Dojindo Molecular Technologies, Inc. Tissue Protein
Extraction Kit (No. KGP2100) was from KEYGEN Biotech. Co., Ltd. (Nanjing, China). ...

[HTML] A novel double-targeted nondrug delivery system for targeting cancer stem cells

S Qiao, Y Zhao, S Geng, Y Li, X Hou, Y Liu, FH Lin… - International Journal of …, 2016

... Cell cytotoxicity assay. Cell cytotoxicity was determined by Cell Counting Kit-8
(CCK-8) (Dojindo, Kumamoto, Japan) according to the manufacturer's instructions.
A total of 5,000 HUVECs were seeded in 96-well plates after 24 ...

[HTML] Bleomycin Suppresses the Proliferation and the Mobility of Human Gastric Cancer Cells Through the Smad Signaling Pathway

J Liu, X Nie, Y Shao, W Su, H Ma, X Xu - Cellular Physiology and Biochemistry, 2016

... Cell proliferation assay. Cell proliferation was assessed by the cell counting kit-8
(CCK-8) assay according to the manufacturer's protocol (Dojindo Laboratories,
Gaithersburg, MD, USA). In brief, 1.0 × 10 4 cells/well were seeded ...

[PDF] Bioinformatic identification of IGF1 as a hub gene in hepatocellular carcinoma (HCC) and in-vitro analysis of the chemosensitizing effect of miR-379 via suppressing …

DJ HUANG, JZ HUANG, J Yang, YH LI, YC LUO… - European Review for …, 2016

... The cells were then treated with varying concentrations of 5-FU, pa- clitaxel (PTX) or Doxorubicin
(DOX) for 72 h. After the treatment, cell viability was measured using the Cell Counting Kit-8
(Dojindo, Tokyo, Japan) according to manufacturer's instruction. ...

[HTML] Triptolide Combined with Radiotherapy for the Treatment of Nasopharyngeal Carcinoma via NF-?B-Related Mechanism

W Zhang, M Kang, T Zhang, B Li, X Liao, R Wang - International Journal of Molecular …, 2016

Advanced nasopharyngeal carcinoma (NPC) has a poor prognosis because of the lack of an
effective treatment. Here we explored the efficiency and the molecular mechanisms of combined
treatment with triptolide and ionizing radiation for treating NPC. Human nasopharyngeal ...

[PDF] Overexpression of CRM1 indicates poor prognosis and its knockdown suppresses tumor growth in gastric cancer

Y Li, H Wang, Q Li, Q Li, Y Gao - Int J Clin Exp Pathol, 2016

... Cell Counting Kit-8 (Dojindo Laboratories, Japan) was used to measure the
absorbance value of OD 450 at 24 h intervals for 5-7 days. All experiments were
independently repeated at least three times. Soft agar growth assay ...

[HTML] PRKAR1A is a functional tumor suppressor inhibiting ERK/Snail/E-cadherin pathway in lung adenocarcinoma

S Wang, Y Cheng, Y Zheng, Z He, W Chen, W Zhou… - Scientific Reports, 2016

... (B) ****P < 0.001. (D,G) CCK-8 (Cell Counting Kit-8) and (E,H) soft ... Cell proliferation assay.
2000cells/well were seeded in 96-well plates. Cell Counting Kit (CCK8) assays were performed
according to the manufacturer's instructions (CK04, Dojindo subsidiary, Shanghai, China). ...

[PDF] Obatoclax inhibits alphavirus membrane fusion by neutralizing the acidic environment of endocytic compartments

FS Varghese, K Rausalu, M Hakanen, S Saul… - Antimicrobial Agents and …, 2016

... For 216 infections done with picornaviruses, cell viability was measured in parallel using the
217 CCK (Cell Counting Kit)-8 (Dojindo) according to manufacturer's instructions. 218 219
Endosomal bypass assay 220 Page 10. Novel antiviral mechanism of obatoclax 10 ...

SYNTHETIC PEPTIDE THAT INCREASES RADIOSENSITIVITY OF TUMOR CELLS AND USE OF SAME

N Baileykobayashi, N Usami, T Yoshida - US Patent 20,160,346,346, 2016

Provided are a synthetic peptide that increases the radiosensitivity of tumor
cells and a pharmaceutical composition containing that synthetic peptide.
<p>The peptide provided by the present invention.

Hepatitis B Virus e Antigen Regulates Monocyte Function and Promotes B Lymphocyte Activation

B Lu, B Zhang, L Wang, C Ma, X Liu, Y Zhao, Y Jiao - Viral Immunology, 2016

... Cell Counting Kit-8 was from Dojindo Laboratorise (Shanghai), Co., Ltd. All primers were
synthesized and validated by Sangon Biotech (Shanghai, China) Co., Ltd. ... CIR cells was
detected by using Cell Counting Kit-8 (Dojindo Laboratorise, co., LTD). ...

Naringenin suppresses Edwardsiella tarda infection in GAKS cells by NanA sialidase inhibition

S Shinyoshi, Y Kamada, K Matsusaki… - Fish & Shellfish Immunology, 2016

... 2.4. Cytotoxicity assay. Cell Counting Kit-8 (DOJINDO, Kumamoto, Japan) was used
for the estimation of cell toxicity. GAKS cells were seeded at 80% confluency in
96-well plate. Various concentrations of test compounds were ...

[HTML] miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke

S Yao, B Tang, G Li, R Fan, F Cao - Neuropsychiatric Disease and Treatment, 2016

... USA). The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories
(Kumamoto, Japan). The Trizol reagent and the M-MLV Reverse Transcriptase kit
were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies ...

Downregulation of DNA methyltransferase 3 alpha promotes cell proliferation and invasion of ectopic endometrial stromal cells in adenomyosis

Y Zou, FY Liu, LQ Wang, JB Guo, BC Yang, XD Wan… - Gene, 2016

... 2.6. Cell proliferation assay. Cell proliferation assay was assessed over six days
by a Cell Counting Kit-8 (CCK-8, Dojindo China Co., Ltd). For the CCK-8 assay,
cells at 72 h post-infection were seeded in a 96-well plate at 6.0 ...

Three-dimensional Hepatic Lobule-like Tissue Constructs Using Cell-microcapsule Technology

Z Liu, M Takeuchi, M Nakajima, C Hu, Y Hasegawa… - Acta Biomaterialia, 2016

... Cell counting kit-8 (CCK8) was purchased from Dojindo Ltd (Tokyo, Japan). Rat
albumin enzyme-linked immunosorbent assay (ELISA) quantitation kit
(ERA3201-1) was purchased from Assaypro Inc (St. Charles, MO, USA). ...

Sulforaphane inhibits osteoclast differentiation by suppressing the cell-cell fusion molecules DC-STAMP and OC-STAMP

T Takagi, H Inoue, N Takahashi, R Katsumata-Tsuboi… - … and Biophysical Research …, 2016

... Cell viability was assessed using the Cell Counting Kit-8 (CCK-8; DOJINDO, Kumamoto, Japan).
The effect of SFN on cell viability was expressed as percent cell viability, with SFN-untreated cells
set at 100%. 2.4. Osteoclast differentiation and TRAP staining assay. ...

[HTML] miR-3940-5p Functions as a Tumor Suppressor in Non–Small Cell Lung Cancer Cells by Targeting Cyclin D1 and Ubiquitin Specific Peptidase-28

K Ren, Y Li, H Lu, Z Li, X Han - Translational Oncology, 2017

... Cell Proliferation Assay. Cell proliferation was determined using Cell Counting Kit 8
(CCK-8; Dojindo, Tokyo, Japan) according to the manufacturer's instructions. Briefly, cells
were seeded in 96-well plates at a density of 3 × 10 3 cells/well. ...

Down-regulation of microRNA-451a facilitates the activation and proliferation of CD4+ T cells by targeting Myc in patients with dilated cardiomyopathy

Z Zeng, K Wang, Y Li, N Xia, S Nie, B Lv, M Zhang… - Journal of Biological …, 2016

... Cell counting kit-8 (CCK-8) assay - CCK-8 assay was performed to assess the proliferation of
Jurkat T cells. ... Then, 10 µl of CCK-8 solution (Dojindo Laboratories, Japan) was added to each
well, and the samples were incubated for 4 hours at 37°C. The absorbance at 450 nm ...

Circular RNA profile identifies circPVT1 as a proliferative factor and prognostic marker in gastric cancer

J Chen, Y Li, Q Zheng, C Bao, J He, B Chen, D Lyu… - Cancer Letters, 2016

... The cells were transfected using Lipofectamine RNAiMax (Life Technologies). 2.9. CCK8 assay.
Cell proliferation was assessed by Cell Counting Kit-8 assay (Dojindo Laboratories, Kumamoto,
Japan). The cells (1 ×10 3 ) were seeded into 96-well plates. ...

[HTML] Differential protein expression in metallothionein protection from depleted uranium-induced nephrotoxicity

Y Hao, J Huang, C Liu, H Li, J Liu, Y Zeng, Z Yang, R Li - Scientific Reports, 2016

... confocal microscope (Leica TCS-SP5, Bensheim, Germany). Cell viability measurement.
Cell activity was monitored using a cell counting kit-8 (CCK-8) (Dojindo, Shanghai,
China) according to the manufacturer's instruction as our.

Glucagon-like peptide-1 inhibits the receptor for advanced glycation endproducts to prevent podocyte apoptosis induced by advanced oxidative protein products

SS Zhang, Z Wu, Z Zhang, ZY Xiong, H Chen… - … and Biophysical Research …, 2016

... AOPPs, advanced oxidative protein products; CCK-8, cell counting kit-8; Bax, Bcl-2-associated
X protein; MCP-1, monocyte chemoattractant protein-1 ... The Cell Counting Kit (CCK)-8 was from
Dojindo Laboratories (Kumamoto, Kyushu, Japan), the Hoechst 33258 Staining Kit was ...

Reduced allergic lung inflammation by root extracts from two species of Peucedanum through inhibition of Th2 cell activation

AR Lee, JM Chun, AY Lee, HS Kim, GJ Gu, BI Kwon - Journal of Ethnopharmacology, 2016

... After washing, stained cells were analyzed by flow cytometry. Cell viability was measured
by using the Cell Counting Kit-8 (CCK-8) assay according to the manufacturer's instructions
(Dojindo Molecular Technologies, Inc., Rockville, MD). 2.12. Statistics. ...

[HTML] Anticancer effect of linalool via cancer-specific hydroxyl radical generation in human colon cancer

K Iwasaki, YW Zheng, S Murata, H Ito, K Nakayama… - World Journal of …, 2016

... All cells were cultured in a 5% CO 2 cell culture incubator at 37 °C. Cell viability was
examined using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan), according
to the manufacturer's instructions. Cancer cells ware seeded ...

IL-22 induced miR-122-5p promotes keratinocyte proliferation by targeting Sprouty2

M Jiang, W Ma, Y Gao, K Jia, Y Zhang, H Liu, Q Sun - Experimental Dermatology, 2016

... negative control as mentioned above. The proliferation rate of HaCaT cells was estimated using
Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Kumamoto, Japan) following the manufacturer's
instructions. According to different time intervals, CCK-8 was added to each ...

Serum-free culturing of human mesenchymal stem cells with immobilized growth factors

H Mao, SM Kim, M Ueki, Y Ito - Journal of Materials Chemistry B, 2017

... mL -1 each) in a medium without FBS. Cell proliferation assay. The proliferation
of the cells was investigated by the WST-8 assay using a Cell Counting Kit-8
(CCK-8, Dojindo, Japan). Briefly, CCK-8 solution was added to the ...

[HTML] Effects of platelet-rich plasma and triamcinolone acetonide on interleukin-1ß-stimulated human rotator cuff-derived cells

T Muto, T Kokubu, Y Mifune, A Inui, R Sakata, Y Harada… - Bone and Joint Research, 2016

Skip to main content. Main menu. Home: Home; About the Journal; Editorial Board; My
Account. Articles: Current Issue; Archive. Specialty: Children's Orthopaedics; Foot & Ankle;
Hip; Knee; Oncology; Research; Shoulder & Elbow; Spine; ...

Evaluation of the osteo-inductive potential of hollow three-dimensional magnesium-strontium substitutes for the bone grafting application

M Li, X Yang, W Wang, Y Zhang, P Wan, K Yang… - Materials Science and …, 2016

... After incubation for 1, 3 and 7 days, 10 µL Cell Counting Kit-8 (CCK-8, DOJINDO, Japan) was
added into each well, followed by continuous incubation for 4 h. The absorbance value at 450
nm was measured in a microplate reader. Each experiment was carried out in triplicate. ...

[HTML] Downregulation of peroxiredoxin-3 by hydrophobic bile acid induces mitochondrial dysfunction and cellular senescence in human trophoblasts

WB Wu, R Menon, YY Xu, JR Zhao, YL Wang, Y Liu… - Scientific Reports, 2016

Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific disorder characterised by
raised bile acids in foetal-maternal circulation, which threatens perinatal health.

[PDF] A novel platform of cardiomyocytes culture and coculture via fibroblast-derived matrix-coupled aligned electrospun nanofiber

M Suhaeri, R Subbiah, SH Kim, CH Kim, SJ Oh… - ACS Applied Materials & …, 2016

Page 1. 1 A novel platform of cardiomyocytes culture and coculture via fibroblast-derived
matrix-coupled aligned electrospun nanofiber Muhammad Suhaeri †‡ , Ramesh Subbiah †‡
, Su-Hyun Kim ? , Chong-Hyun Kim ? , Seung Ja Oh † , ...

[HTML] Sharpin promotes hepatocellular carcinoma progression via transactivation of Versican expression

Y Tanaka, K Tateishi, T Nakatsuka, Y Kudo… - Oncogenesis, 2016

... Briefly, cells were seeded in 96-well plates at a density of 4 × 10 3 cells per well. The number
of viable cells in triplicate wells was determined using the Cell Counting Kit-8 (Dojindo Molecular
Technologies, Rockville, MD, USA). Lentivirus-mediated introduction of transgene. ...

Gender differences of B cell signature related to estrogen-induced IFI44L/BAFF in systemic lupus erythematosus

H Fan, G Zhao, D Ren, F Liu, G Dong, Y Hou - Immunology Letters, 2017

... 2.10. Cell viability analysis. Cell viability was assessed with a Cell Counting Kit 8
(Dojindo Laboratories Inc., Mashikimachi, kamimashiki gun, Japan), according to the
recommendations of the manufacturer. 2.11. Statistical analysis. ...

The novel antioxidant TA293 reveals the role of cytoplasmic hydroxyl radicals in oxidative stress-induced senescence and inflammation

T Sakai, J Imai, T Ito, H Takagaki, M Ui, S Hatta - Biochemical and Biophysical …, 2016

... 2.9. Cell proliferation and cytotoxicity assays. Cytotoxic effects were evaluated using a Cell
Counting Kit-8 (Dojindo, Kumamoto, Japan). We harvested cells and plated them in 96-well plates
at 1 × 10 3 cells/well. The cells were maintained at 37 °C in a humidified incubator. ...

[HTML] Screening of long non-coding RNA and TUG1 inhibits proliferation with TGF-ß induction in patients with COPD

W Tang, Z Shen, J Guo, S Sun - … Journal of Chronic Obstructive Pulmonary Disease, 2016

... Cell Counting Kit-8 assay was used to detect BEAS-2B and HFL1 cell proliferation after
TUG-siRNA transfection with TGF-ß treatment. ... well plates at 1×10 4 cells per well at 37°C. At 24,
48, and 72 h, 10 µL of cholecystokinin octapeptide (CCK-8; Dojindo Laboratories, Kumamoto ...

[HTML] Let-7a enhances the sensitivity of hepatocellular carcinoma cells to cetuximab by regulating STAT3 expression

F Xue, Y Liu, H Zhang, Y Wen, L Yan, Q Tang, E Xiao… - OncoTargets and therapy, 2016

... CO 2 for 48 hours. Cell viability was subsequently measured using a Cell Counting
Kit-8 (CCK-8, Dojindo Laboratories, Tokyo, Japan) after 1–2 hours, according to
manufacturer's instructions. An MRX II microplate reader (Dynex ...

Ion Release, Microstructural, and Biological Properties of iRoot BP Plus and ProRoot MTA Exposed to an Acidic Environment

J Tian, Y Zhang, Z Lai, M Li, Y Huang, H Jiang, X Wei - Journal of Endodontics, 2016

... were placed into 96-well plates. After 1, 3, and 5 days in culture, 10 µL Cell Counting
Kit-8 (Dojindo, Kumamoto, Japan) was added to each well followed by incubation
at 37°C for 2 hours. To assay cell adhesion, MC3T3-E1 ...

Elevated TARP promotes proliferation and metastasis of salivary adenoid cystic carcinoma

H Yue, Y Cai, Y Song, L Meng, X Chen, M Wang… - Oral Surgery, Oral Medicine, …, 2016

... Cell proliferation was determined using the Cell Counting Kit-8 assay. ... At the same time each
day for seven consecutive days, after 24 h of incubation, the medium was removed and a mixture
of 30 µL CCK-8 (Dojindo, Kyushu, Japan) and 270 µL of RPMI 1640 medium was ...

Inhibiting PI3K-AKt signaling pathway is involved in antitumor effects of ginsenoside Rg3 in lung cancer cell

Q Xie, H Wen, Q Zhang, W Zhou, X Lin, D Xie, Y Liu - Biomedicine & …, 2017

... DMSO was added as a control group and the maximum final concentration of DMSO was <0.1%
for each group. Cell Counting kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto,
Japan). Annexin V/FITC kit was purchased from Vazyme Biotech Co., Ltd. ...

Down-regulated long non-coding RNA-ATB in preeclampsia and its effect on suppressing migration, proliferation, and tube formation of trophoblast cells

X Liu, H Chen, W Kong, Y Zhang, L Cao, L Gao… - Placenta, 2016

... 3.4. Cell proliferation assay. Cell proliferation assays were performed with Cell Counting
Kit-8 (DOJINDO, Japan) according to the manufacturer's protocol, and detected at 0, 24,
48, and 72 h post-transfection. 3.5. Cell colony formation assay. ...

Incorporation of cerium oxide into hydroxyapatite coating regulates osteogenic activity of mesenchymal stem cell and macrophage polarization

K Li, Q Shen, Y Xie, M You, L Huang, X Zheng - Journal of Biomaterials Applications, 2016

... The quantitative analysis of BMSCs proliferation on HA-CeO 2 coating surfaces was conducted
using the Cell Counting Kit-8 (CCK-8, Dojindo Molecular Technology, Sigma, Japan) and 2 ×
10 4 cells/well were seeded on the coating surfaces (ø 10 mm). ...

Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta

S Li, A Gao, S Dong, Y Chen, S Sun, Z Lei, Z Zhang - International Journal of …, 2016

... streptomycin solution were purchased from Sigma Aldrich, Inc. (Saint Louis, MO, USA).
A cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies,
Inc. (Kumamoto, Japan). 2.2. Preparation of polysaccharides. ...

Trichostatin A increases radiosensitization of tongue squamous cell carcinoma via miR-375

L Jia, S Zhang, Y Huang, Y Zheng, Y Gan - Oncology Reports, 2017

... Cell proliferation assays. Cell proliferation was measured using Cell Counting Kit-8
(CCK-8, Dojindo, Kumamoto, Japan). Briefly, the cells were plated onto 96-well plates
(2x103 cells/well). After treatment with TSA and/or exposure ...

[HTML] Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery

W Zhao, Y Zhang, X Jiang, C Cui - Drug Design, Development and Therapy, 2016

... The cytotoxicity of the vector was measured by Cell Counting Kit-8 (CCK-8) assay, and the
targeting efficiency and tumor cell viability were evaluated in vivo. ... (Logan, UT, USA). CCK-8
was purchased from Dojindo Molecular Technologies (Osaka, Japan). ...

[HTML] A specific DNA-nanoprobe for tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells

J Zhai, Y Liu, S Huang, S Fang, M Zhao - Nucleic Acids Research, 2016

... Hoechst 33342, propidium iodide (PI) and cell-counting kit (CCK-8) were all obtained from Dojindo
Laboratories (Kumamoto, Japan). ... The cytotoxicity of Nanoprobe A was also examined by using
the Cell Counting Kit-8 (CCK-8) and proved to be very low (Supplementary Figure ...

[HTML] Overexpression of PBK/TOPK Contributes to Tumor Development and Poor Outcome of Esophageal Squamous Cell Carcinoma

T OHASHI, S KOMATSU, D ICHIKAWA, M MIYAMAE… - Anticancer Research, 2016

... For measurements of cell growth, the number of viable cells at different time points after
transfection was assessed by the colorimetric water-soluble tetrazolium salt (WST) assay
(Cell counting kit-8; Dojindo Laboratories, Kumamoto, Japan). ...

Activating AKT to inhibit JNK by troxerutin antagonizes radiation-induced PTEN activation

P Xu, WB Zhang, XH Cai, PY Qiu, MH Hao, DD Lu - European Journal of …, 2016

... Scientific Inc. (Waltham, MA USA). Cell counting kit-8 (CCK-8) was manufactured
by Dojindo Laboratories (Kumamoto, Japan). Apoptosis kit was obtained from
Bao-Sai Biotechnology Co., Ltd, Beijing, China. RPMI-1640 and ...

[PDF] Rab14 Suppression Mediated by MiR-320a Inhibits Cell Proliferation, Migration and Invasion in Breast Cancer

J Yu, L Wang, H Yang, D Ding, L Zhang, J Wang… - Journal of Cancer, 2016

... Relative quantification was determined by the 2-??CT method [21]. Cell proliferation
Cell proliferation was determined by Cell Counting Kit-8 assay (Dojindo, Japan)
according to the protocol. Cells were plated in 96-well plates. ...

[HTML] Intravenous hMSCs Ameliorate Acute Pancreatitis in Mice via Secretion of Tumor Necrosis Factor-a Stimulated Gene/Protein 6

Z He, J Hua, D Qian, J Gong, S Lin, C Xu, G Wei… - Scientific Reports, 2016

... Furthermore, we also evaluated the proliferation of acinar cells using a cell counting
Kit-8 (CCK-8; Dojindo, Kumamoto, Japan) according to the manufacturer's instructions.
Transfection of pancreatic acinar cells with CD44 siRNA. ...

[HTML] Thermo-sensitive hydrogels combined with decellularised matrix deliver bFGF for the functional recovery of rats after a spinal cord injury

HL Xu, FR Tian, CT Lu, J Xu, ZL Fan, JJ Yang… - Scientific Reports, 2016

... UK). Fluorescein isothiocyanate (FITC) was ordered from Sigma (Sigma-Aldrich,
St. Louis, MO, USA). Cell Counting KIT-8 was purchased from Japan (CCK-8, Dojindo
Laboratories Inc., Kumamoto, Japan). Animal and cells. ...

DANCR Acts as a Diagnostic Biomarker and Promotes Tumor Growth and Metastasis in Hepatocellular Carcinoma

X MA, XIN WANG, C YANG, Z WANG, B HAN, L WU… - Anticancer Research, 2016

... Cell proliferation assays. Cell Counting Kit-8 (CCK-8, Dojindo, Kumamoto, Japan)
was used to evaluate cell proliferation. Briefly, cells were seeded into a 96-well plate
and transfected with corresponding oligonucleotides. At ...

Tetramethylpyrazine protects against glucocorticoid-induced apoptosis by promoting autophagy in mesenchymal stem cells and improves bone mass in glucocorticoid …

L Wang, HY Zhang, B Gao, J Shi, Q Huang, YH Han… - Stem Cells and …, 2016

... 100 µM, 200 µM, or 1 mM) for 24 h or 72 h to test the toxicity of TMP. A Cell Counting
Kit-8 (CCK-8; Dojindo Molecular Technologies, Kumamoto, Japan) was used to measure
cell viability in accordance with the manufacturer's instructions, and ...

[PDF] MicroRNA-1284 enhances radio-sensitivity in hepatocellular carcinoma cells by regulating SP1

H Zhang, X Sun, L Ma - Int J Clin Exp Pathol, 2016

... 1284 mimic or control was transfected into cells, and transfected cells viability and radio-sensitivity
were measured by Cell Counting Kit-8 (CCK-8 ... Cell viability was determined by using Cell Co-
unting Kit-8 (CCK-8; Dojindo, Kumamoto Pre- fecture, Kyushu, Japan) according to ...

[HTML] Metformin Inhibits TGF-ß1-Induced Epithelial-to-Mesenchymal Transition via PKM2 Relative-mTOR/p70s6k Signaling Pathway in Cervical Carcinoma Cells

K Cheng, M Hao - International Journal of Molecular Sciences, 2016

... 10 mM metformin with or without 10 ng/mL TGF-ß1. The Cell Counting Kit-8
(CCK-8, Dojindo, Tokyo, Japan) assays were performed to determine the proliferation
of cells. As shown in Figure 2A and Figure 3A, metformin led ...

GADD45a is involved in the apoptosis of lymphocytes induced by riboflavin and ultraviolet light

P Yang, H Wen, T Zhong, H Hu, B Zhu, K Xia, M Xu… - Transfusion, 2016

... well plate, and the cells were incubated for 2 hours at 37°C with 5% CO 2 . Lymphocyte
proliferation was determined at the indicated time points using the cell counting kit-8 (CCK-8)
Assay kit (Dojindo Molecular Technologies, Inc.) according to the manufacturer's instructions. ...

[HTML] LSD1/KDM1 isoform LSD1+ 8a contributes to neural differentiation in small cell lung cancer

T Jotatsu, S Yagishita, K Tajima, F Takahashi… - … and Biophysics Reports, 2016

... Cell proliferation assays were performed at 24, 48, and 72 h using WST-8 (cell counting kit-8,
Dojindo Laboratories, Kumamoto, Japan). ... After 72 h or 7 days of incubation, 10 µl of WST-8 (cell
counting kit-8, Dojindo Laboratories, Kumamoto, Japan) was added to each well. ...

[HTML] Effect of N-Perfluorooctane on Hypoxia/Reoxygenation Injury in Human Umbilical Vein Endothelial Cells

J Jiang, DY Chen, ZT Liu, F Chen, JJ Zhang, J Cui… - Acta Cardiologica Sinica, 2016

... Cell viability A values in each group by cell counting kit-8 (CCK-8) assay. ... After the experimental
treatments, 10 µL of CCK-8 solution was added to each well (Dojindo Laboratories, Japan), and
cells were incubated for 1 h. They were estimated by absorptiometry through the ...

[HTML] STAT6 deficiency ameliorates Graves' disease severity by suppressing thyroid epithelial cell hyperplasia

X Jiang, B Zha, X Liu, R Liu, J Liu, E Huang, T Qian… - Cell Death & Disease, 2016

... Nthy-ori 3-1 cell viability was measured using a Cell Counting Kit-8 (CCK-8; Dojindo,
Kunamoto, Japan) based on a redox assay similar to the 3-(4,5)-dimethylthiahiazo
(-z-y1)-3,5-di- phenytetrazoliumromide assay. Nthy-ori 3-1 cell inhibition assay. ...

[HTML] Tumor Necrosis Factor-Like Weak Inducer of Apoptosis Promotes Hepatic Stellate Cells Migration via Canonical NF-?B/MMP9 Pathway

M Xu, F Zhang, A Wang, C Wang, Y Cao, M Zhang… - PloS one, 2016

... Transwell chambers (pore size 8um) were purchased from BD Biosciences, USA. Cell
Counting Kit-8 (CCK-8) kit was purchased from DOJINDO Laboratories, Japan. MMP9
and p65 siRNA were acquired from RiboBio (Guangdong,China). ...

[HTML] Endocytosed nanoparticles hold endosomes and stimulate binucleated cells formation

L Xia, W Gu, M Zhang, YN Chang, K Chen, X Bai, L Yu… - Particle and Fibre …, 2016

... After 24 or 48 h, cell viability was measured using Cell Counting Kit (CCK-8) ((Dojindo
Laboratories, Japan) according to the manufacturer's instruction. ... Bicinchoninic acid. BSA: Bovine
serum albumin. CCK-8: Cell counting kit-8. DMEM: Dulbecco's minimal essential media. EEA1 ...

[PDF] Albumin conjugated lipid nanoemulsion for site specific delivery of rapamycin at inflammatory site of spinal cord injury

TY Geng, C Xu, GH Xu - Int J Clin Exp Med, 2016

... Ltd.; Dulbecco's modi- fied Eagle's medium was from Invitrogen; Cell Counting KIT-8 was from
Dojindo Laboratories Inc.; Alcohol, Hematoxylene, Eosin, Dpx mount, and Xylene were from
Thomas Baker Pvt. Ltd.; TNF-a and IL-1ß ELISA kit were from Ther- mo fisher scientific. ...

Synthesis of Nontoxic Fluorous Sphingolipids as Molecular Probes of Exogenous Metabolic Studies for Rapid Enrichment by FSPE

S Saito, Y Murai, S Usuki, M Yoshida, MAS Hammam… - European Journal of …, 2016

... After 24 hours, 10µl of Cell Counting Kit-8 Solution (obtained from Dojindo Molecular
Technologies, Inc.) was added. After 4 hours, Absorbance of visible light (wavelength: 450nm)
was measured and cell viability was calculated as following formula: ...

[PDF] MicroRNA-206 acts as a tumor suppressor in bladder cancer via targeting YRDC

B Huang, W Zhai, G Hu, C Huang, T Xie, J Zhang, Y Xu - Am J Transl Res, 2016

... Cell proliferation and colony formation assay Cells proliferation was assessed using
the Cell Counting Kit-8 (CCK-8, Dojindo, Kumamoto, Japan). Briefly, the transfected
cells were seed- ed into 96-well plates at a density of ...

Activation of EVI1 transcription by the LEF1/ß-catenin complex with p53-alteration in myeloid blast crisis of chronic myeloid leukemia

N Manachai, Y Saito, S Nakahata, AG Bahirvani… - … and Biophysical Research …, 2016

... 2.2. Reagents and drugs treatment. The transfection reagent HilyMax and the cell
proliferation/cell toxicity Cell Counting Kit-8 were purchased from DOJINDO (Kumamoto, Japan).
Lithium chloride anhydrous was purchased from Nacalai Tesque (Kyoto, Japan). ...

Development of pH-responsive starch–glycol chitosan nanogels for proapoptotic (KLAKLAK) 2 peptide delivery

SK Kim, YS Youn, KT Oh, ES Lee - Journal of Bioactive and Compatible Polymers: …, 2016

... Mitotracker® was purchased from Invitrogen Inc. (Waltham, MA, USA). The BCA protein assay
kit was obtained from Pierce (Milwaukee, WI, USA). A Cell Counting Kit-8 (CCK-8) was obtained
from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan).7–10,14–16 Page 3. ...

[HTML] GANT61, a GLI inhibitor, sensitizes glioma cells to the temozolomide treatment

J Li, J Cai, S Zhao, K Yao, Y Sun, Y Li, L Chen, R Li… - Journal of Experimental & …, 2016

... Cell viability assay. The cytotoxicity of TMZ and GANT61 on U87 and U251 cells
was determined by Cell Counting Kit-8 (CCK-8, Dojindo, Japan) assay. Tumor cells
were seeded at 2-5 × 10 3 cells/well (0.1 ml) in 96-well flat ...

[HTML] Endocan as a prognostic biomarker of triple-negative breast cancer

A Sagara, K Igarashi, M Otsuka, A Kodama… - Breast Cancer Research …, 2016

... All cells were maintained under a humidified atmosphere of 5% CO 2 at 37 °C. Cell
proliferation assay. The proliferation rate for each cell line was determined using a Cell
Counting Kit-8 (CCK-8; Dojindo Laboratories, Kumamoto, Japan). ...

[HTML] Polyethyleneimine-coated quantum dots for miRNA delivery and its enhanced suppression in HepG2 cells

G Liang, Y Li, W Feng, X Wang, A Jing, J Li, K Ma - International Journal of …, 2016

... Untreated cells were taken as control with 100% viability. Cell Counting Kit-8 (CCK-8; Dojindo
Molecular Technologies, Kumamoto, Japan) was used for assessing the cytotoxicity of
PEI/QD. Details of the process are provided in manufacturer's instruction. ...

[PDF] Deep phosphotyrosine proteomics by optimization of phosphotyrosine enrichment and MS/MS parameters

Y Abe, M Nagano, A Tada, J Adachi, T Tomonaga - Journal of Proteome Research, 2016

... Japan). An ImmunoStar LD kit was obtained from Wako (Osaka, Japan). Cell Counting
Kit-8 was purchased from Dojindo (Kumamoto, Japan). The Prism 6 software package
was obtained from GraphPad Software (La Jolla, CA, USA). ...

Alkalescent nanotube films on a titanium-based implant: A novel approach to enhance biocompatibility

Y Zhang, C Dong, S Yang, J Wu, K Xiao, Y Huang, X Li - Materials Science and …, 2016

... To characterize the adhesion and proliferation of MC3T3-E1, a cell counting kit-8 assay
(CCK-8, Dojindo, Japan) was used to quantitatively evaluate the number of cells. Because
the WST–8 in the assay can be reduced by dehydrogenase ...

[HTML] Lentivirus-mediated knockdown of NLK inhibits small-cell lung cancer growth and metastasis

M Lv, Y Li, X Tian, S Dai, J Sun, G Jin, S Jiang - Drug Design, Development and …, 2016

... 1–5 days. At each termination of culture, cell viability analysis was performed by
a Cell Counting Kit-8 (CCK-8) assay according to the manufacturer's instructions
(Dojindo Laboratories, Kumamoto, Japan). After an incubation ...

PDK1 promotes tumor cell proliferation and migration by enhancing the Warburg effect in non-small cell lung cancer

T Liu, H Yin - Oncology Reports

... Cell Counting Kit-8 (CCK-8, Dojindo, Japan) was used to measure the cell viability at 24, 48,
72 and 96 h according to the manufacturer's insturctions. The absorbance was tested at 450 nm.
The experiments were repeated at least three times. Colony formation assay. ...