Cell Counting Kit-8
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December 2017 Publications~

[PDF]Angiopoietin-1 alleviates LPS-induced inflammatory injury by up-regulation of miR-126 in pancreas cell line HPDE6-C7

B Liu, Y Sun, J Xu, X Wang, Y Sun - Int J Clin Exp Pathol, 2017

… Cell proliferation was assessed by a cell count- ing kit-8 (CCK-8, Dojindo Molecular Technolo-
gies, Gaithersburg, MD) … Ang-1: Angiopoietin-1; CCK-8: cell counting kit-8; ELISA: enzyme-linked
immunosorbent assay; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; IL …

[HTML]NRBP2 Overexpression Inhibits Cell Proliferation and Migration and Increases Cisplatin Sensitivity in Intrahepatic Cholangiocarcinoma

X Li, X Chen, J Jiang, S Yang, Y Gao - Technology in Cancer Research & Treatment, 2017

Aims: Nuclear receptor binding protein 2 is ubiquitously expressed in all tissues in humans.
However, few studies have reported the function of nuclear receptor...

[PDF]Fisetin suppresses malignant proliferation in human oral squamous cell carcinoma through inhibition of Met/Src signaling pathways

YS Li, XJ Qin, W Dai - Am J Transl Res, 2017

… containing 10% FBS. Cells were treated with the indicated concentrations of fisetin
for 24 h. The cell Counting Kit-8 (CCK8, Dojindo Molecular Technologies, Inc., Japan)
was used to evaluate cell proliferation. Generally, 10 µl …

[PDF]Ursodeoxycholic acid induces autophagy via LC3B to suppress hepatocellular carcinoma in vivo and in vitro

F Wang, C Qin, Y Li, W Qu, H Liu, B Li, J Qi, J Liu… - Int J Clin Exp Pathol, 2017

… medium). We detected the absorbance at 24 h, 48 h and 72 h after UDCA treatment
using a Cell Counting Kit-8 (CCK-8) cell viability assay (Dojindo, Japan) according
to the manufactur- er's instructions. Cell migrationassays …

Triptolide inhibits pituitary adenoma cell viability, migration and invasion via ADAM12/EGFR signaling pathway

J Wang, Z Zhang, R Li, W Sun, J Chen, H Zhang, K Shu… - Life Sciences, 2017

… Subsequently, Cell Counting Kit 8 (CCK-8, Dojindo Laboratories, Kumamoto, Japan) solution
were added into each well and incubated for 2 h at 37 °C in 5% CO 2 . Absorbance at 490 nm
was assessed in a plate reader (Tecan Group Ltd., Männedorf, Switzerland). 2.5 …

[PDF]Silencing CCAT2 inhibited proliferation and invasion of epithelial ovarian carcinoma cells by regulating Wnt signaling pathway

BD Wang, J Jiang, MM Liu, RJ Zhuang, H Wang, PL Li - Int J Clin Exp Pathol, 2017

… Next, cells were har- vested for other experiments. Cell proliferation and colony formation assays
The Cell Counting Kit-8 Kit (CCK-8 Kit, Dojindo Laboratories, Japan) was used to determine
relative cell growth according to the instruc- tions of the manufacturer …

[PDF]The long non-coding RNA SUMO1P3 facilitates breast cancer progression by negatively regulating miR-320a

J Liu, Z Song, C Feng, Y Lu, Y Zhou, Y Lin, C Dong - Am J Transl Res, 2017

… Following transfection with sh-SUMO1P3 alone or with sh-SUMO1P3 and miR-320a or with sh-
SUMO1P3 and si-NC, cell proliferation was assessed using the Cell Counting Kit-8 (CCK-8;
Dojindo Molecular technologies, Inc., Kyushu, Japan) according to the manufacturer's …

Diethylstilbestrol regulates mouse gubernaculum testis cell proliferation via PLC-Ca2+-CREB pathway

X Zhang, H Ping, J Li, S Duan, X Jiang - Cell Biochemistry and Function, 2017

… Cell proliferation was detected by using Cell Counting Kit-8 (Dojindo, Gaithersburg, MA, USA)
according to the manufacturer's instructions. Absorbance was measured at 450 nm by using a
microplate reader (Molecular Devices, Sunnyvale, CA, USA). 2.4 | Western blot analysis …

Downregulation of long noncoding RNA H19 contributes to the proliferation and migration of papillary thyroid carcinoma

X Lan, W Sun, W Dong, Z Wang, T Zhang, L He… - Gene, 2017

… 2.5. Cell proliferation assay. Cell proliferation was assessed using the cell counting kit-8
(CCK-8, Dojindo, Japan) assay according to the manufacturer's protocol. Briefly, 3000 cells/well
were placed into 96-well plate. An aliquot of 10 µl CCK-8 solution was added to each well …

Efficacy of Lenvatinib, a multitargeted tyrosine kinase inhibitor, on laser-induced CNV mouse model of neovascular AMD

X Wei, T Zhang, Y Yao, S Zeng, M Li, H Xiang, C Zhao… - Experimental Eye Research, 2017

… 1.1.1.1. Proliferation assay of HCMECs. Cell Counting Kit-8 (CCK-8, Dojindo, Japan)
was used for proliferation activity assay as described in previous study (Wiegering
et al., 2014). When HCMECs were at 80% confluence after …

[PDF]Rhodiola crenulata Attenuates Gamma-Ray Induced Cellular Injury Via Modulation of Oxidative Stress in Human Skin Cells

KT Lin, TC Chang, FY Lai, CS Lin, HL Chao, SY Lee - The American Journal of …, 2018

… Cell Viability Cell viability was assessed as described in our previous study (Lee et al.,
2015). The assay was performed using a cell counting kit-8 (CCK-8, Dojindo, Japan).
Briefly, 5 Â 10 3 cells were seeded into 96-well plates overnight …

QUINAZOLINE HETEROCYCLIC COMPOUND AS EGFR KINASE INHIBITOR AND PREPARATION AND APPLICATION THEREOF

W Sheng, L Yang, Z Pan - US Patent 20,170,355,683, 2017

The present invention relates to an N-substituted-phenyl-5-substituted-alkoxy-
2,3-dihydro-[1,4]dioxane[2,3-f]quinazolin-10-amine (I) or 4-substituted-
arylamino-6-substituted-alkyl-6H-[1,4]oxazino[3,2-.

Pyridopyrimidinone compounds for modulating the catalytic activity of histone lysine demethylases (KDMs)

M Kim, T Park, T Yoon, SM Yang, H Kim, JG Kim - US Patent 9,834,550, 2017

The present invention provides a compound of Formula (I) being capable
of modulating the activity of histone lysine demethylase (KDM), pharmaceutical
compositions thereof, methods to prepare the said.

FIBER STRUCTURE FOR USE AS CELL SCAFFOLD MATERIAL

K Kadowaki, C Kuga, M Fujita, K Tanahashi, S Yamada… - US Patent 20,170,342,376, 2017

… hours. After rinsing the well with PBS(-) (manufactured by Nissui Pharmaceutical
Co., Ltd.), 1 mL of a medium was added thereto, followed by addition of 100 µL of
Cell Counting Kit-8 (manufactured by Dojindo Laboratories). The …

Icariin protects against ischemia-reperfusion injury in H9C2 cells by upregulating heat shock protein 20

ZH Ren, ZP Ke, M Luo, Y Shi - Molecular Medicine Reports, 2018

… Following ICA treatment for 48 h, 10% Cell Counting-kit 8 (CCK-8; CK04; Dojindo Molecular
Technologies, Inc., Kumamoto, Japan) diluted in DMEM was mixed in each well for another 1
h. The absorption of each sample was measured at a wavelength of 450 nm using a …

Knockdown of GRHL3 inhibits activities and induces cell cycle arrest and apoptosis of human colorectal cancer cells

X Wang, F Zhou, H Tao, X Wang, C Zhang, F Su… - Journal of Huazhong …, 2017

… blotting, respectively. 1.4.2 CCK-8 Assay After transfection for 48 h, 3×103 CRC cells
per 100 µL medium were seeded in 96-well plates to grow for another 4 days. Then
10 µL Cell Counting Kit-8 (Dojindo Kumamoto, Japan) re …

[PDF] CRISPR/Cas9 targeting of the androgen receptor suppresses the growth of LNCaP human prostate cancer cells

C Wei, F Wang, W Liu, W Zhao, Y Yang, K Li, L Xiao… - Molecular Medicine Reports, 2018

… A Cell Counting kit (CCK)-8 assay (Dojindo Molecular Technologies, Inc., Kumamoto, Japan)
was used to assess the … The Cell Counting kit-8 assay demonstrated that knocking down the
androgen receptor using the clustered regularly interspaced short palindromic repeats …

[PDF] Carnosic acid, an inducer of NAD (P) H quinone oxidoreductase 1, enhances the cytotoxicity of ß-lapachone in melanoma cell lines

N Arakawa, A Okubo, S Yasuhira, K Takahashi… - Oncology Letters, 2018

… (Kumamoto, Japan). Cell viability assay. A Cell Counting kit-8 (Dojindo Mole- cular
Technologies, Inc.), which utilizes water-soluble tetrazolium salts, was used to evaluate
the proliferation of melanoma cells following drug treatment …

[PDF] MicroRNA-638 inhibits cell growth and tubule formation by suppressing VEGFA expression in human Ewing Sarcoma cells

X Zhou, J Chen, Q Xiao, T Wang, Y Yu, B Li, G Shao… - Bioscience Reports, 2017

… This experiment was repeated three times. Cell proliferation assay The cell proliferation was
analyzed using the Cell Counting Kit-8 (CCK-8; Dojindo, Japan). Approximately 5 × 103
cells/well in 100 ul culture medium were plated in 96-well plates for 0h, 24h, 48h or 72h …

Overexpression of CircRNA BCRC4 regulates cell apoptosis and MicroRNA-101/EZH2 signaling in bladder cancer

B Li, F Xie, F Zheng, G Jiang, F Zeng, X Xiao - … of Huazhong University of Science and …, 2017

… 1.4 Cell Viability by CCK-8 Assay Cell viability was determined by the cell counting
kit-8 assay (CCK-8; Dojindo Laboratories, Japan). Cells were plated at a density of 5000
cells per well in 96-well plates, and then allowed to adhere overnight …

Gas chromatography-mass spectrometry metabolomic study of lipopolysaccharides toxicity on rat basophilic leukemia cells

F Cui, P Zhu, J Ji, I Blaženovic, M Gholami, Y Zhang… - Chemico-Biological …, 2017

… 2.3. Cell viability assays and apoptosis. To evaluate the effect of LPS on RBL-2H3
cells growth and viabilities were measured using a Cell Counting Kit-8 (CCK-8;
Dojindo, Kumamoto, Japan). Briefly, this procedure consisted …

EDTA Treatment for Sodium Hypochlorite–treated Dentin Recovers Disturbed Attachment and Induces Differentiation of Mouse Dental Papilla Cells

K Hashimoto, N Kawashima, S Ichinose, K Nara… - Journal of Endodontics, 2017

… software (Image J; National Institutes of Health, Bethesda, MD) (20). The viability of
cells on the disks was measured using a water soluble tetrazolium salt assay (Cell
Counting Kit-8, Dojindo Laboratories). Ultrastructural Analysis …

[HTML] LncRNA-DANCR contributes to lung adenocarcinoma progression by sponging miR-496 to modulate mTOR expression

Q Lu, Z Rui, Z Guo, W Xie, S Shan, T Ren - Journal of Cellular and Molecular …, 2017

… Page 3. Cell proliferation assay The cell proliferation rate was detected using Cell
Counting Kit-8 (CCK- 8; Dojindo, JPN) and 5-ethynyl-20-deoxyuridine assays (EdU;
Ribobio, Guangzhou, China). A549 and H1299 cells were …

Inhibitory activity of linarin on osteoclastogenesis through receptor activator of nuclear factor ?B ligand-induced NF-?B pathway

J Wang, B Fu, F Lu, X Hu, J Tang, L Huang - Biochemical and Biophysical Research …, 2017

… USA). The Cell Counting Kit-8 (CCK-8) was bought from Dojindo Molecular
Technologies (Tokyo, Japan). Antibodies against NFATc1, p65, p-p65and ß-actin
were obtained from Cell Signaling Technology (Beverly, MA, USA) …

[HTML] Anti-adipogenic effects of the traditional herbal formula Dohongsamul-tang in 3T3-L1 adipocytes

EH Shim, H Lee, MS Lee, S You - BMC Complementary and Alternative Medicine, 2017

… Louis, MO, USA) and the cell counting kit-8 (CCK-8) was purchased from Dojindo Laboratories
(Kumamoto, Japan). Trigliceride (TG) kit was obtained from Bioassay Systems (CA, USA) and
leptin ELISA kit was purchased from R&D System Inc. (MI, USA) …

[HTML] MicroRNA-363 inhibits ovarian cancer progression by inhibiting NOB1

Y Lin, T Xu, S Zhou, M Cui - Oncotarget, 2017

… We determined cell proliferation using the Cell Counting Kit-8(CCK8) assays. Briefly, 2 × 10 3
transfected cells were seeded into 96-well plates and cultured at 5% CO 2 and 37°C for 24 -72
h. At each time point, 10µl of CCK-8 solution(Dojindo Laboratories, Kumamoto, Japan …

[HTML] Long noncoding RNA TUG1 is a diagnostic factor in lung adenocarcinoma and suppresses apoptosis via epigenetic silencing of BAX

H Liu, G Zhou, X Fu, H Cui, G Pu, Y Xiao, W Sun… - Oncotarget, 2017

… Cell viability assay. Cell viability was evaluated using a Cell Counting Kit-8 kit (CCK8, Dojindo,
Japan). Briefly, 5×10 3 cells per well were seeded into a 96-well plate. After transfection, the cells
were maintained and the viability was detected at different time points …

[PDF] Targeting LncRNA-MALAT1 suppresses the progression of osteosarcoma by altering the expression and localization of ß-catenin

ZC Zhang, C Tang, Y Dong, J Zhang, T Yuan, XL Li - Journal of Cancer, 2018

… After electrophoresis at 150 V for 40 minutes, the gel was photographed using a Gel Doc™
XR+ system (Bio-Rad). Cell Counting Kit-8 (CCK-8) assay Cell counting kit-8 was purchased
from Dojindo Molecular Technologies (Kumamoto, Japan) …

[HTML] Comprehensive two-dimensional APTES-decorated MCF7-cell membrane chromatographic system for characterizing potential anti-breast-cancer components from …

XY Wang, X Ding, YF Yuan, LY Zheng, Y Cao, ZY Zhu… - Journal of Food and Drug …, 2017

… Acetonitrile and formic acid of HPLC grade were purchased from Merck Co., (Darmstadt, Germany).
While all the other reagents were of analytical grade. The cell counting kit-8 (CCK-8) and cell
apoptosis kit were purchased from Dojindo China CO., Ltd (Shanghai, China) …

Inhibition of the receptor for advanced glycation promotes proliferation and repair of human periodontal ligament fibroblasts in response to high glucose via the NF-?B …

D Zhan, L Guo, L Zheng - Archives of Oral Biology, 2017

… We used the Cell Counting Kit-8 (CCK-8) to assay cell proliferation. hPDLFs at passage 4 were
seeded in 96-well plates at a density of 1 × 104 cells/well. 10 µL of the CCK-8 solution (DOJINDO,
Kyushu, Japan) was added to the media at 24 h, 48 h, and 72 h, followed by an …

[PDF] Overexpression of centrosomal protein 55 regulates the proliferation of glioma cell and mediates proliferation promoted by EGFRvIII in glioblastoma U251 cells

H Zhu, D Chen, J Tang, C Huang, S Lv, D Wang, G Li - Oncology Letters, 2018

… Proliferation assay of U251 cells The Cell Counting Kit-8 (CCK-8) assay. U251 cells (3,000
cells/well) were seeded into 96-well plates in 100 µl complete medium. The CCK-8 (Dojindo
Laboratories, Kumamoto, Japan) was used to measure cell viability accor ding to the …

[HTML] High Tim-3 expression on AML blasts could enhance chemotherapy sensitivity

L Xu, J Xu, S Ma, X Li, M Zhu, S Chen, Y Han, X Tang… - Oncotarget, 2017

… Cell proliferation was determined by a Cell Counting Kit-8 assay (Dojindo, Japan) 48h
after being seeded as instructed by the manufacturer. The absorbance was measured
at 450 nm in a microplate reader (Bio-Tek, Winooski, VT, USA) …

[HTML] LncRNA MALAT1 acts as an oncogene in multiple myeloma through sponging miR-509-5p to modulate FOXP1 expression

Y Gu, X Xiao, S Yang - Oncotarget, 2017

… Cell proliferation assay. The cell proliferation rate was detected using Cell Counting Kit-8 assays
(CCK-8, Dojindo) according to the instructions. 100 ul cell suspension (1×10 3 cells/well) was
seeded in a 96-well plate and various concentrations of substances were added …

Docetaxel-mediated autophagy promotes chemoresistance in castration-resistant prostate cancer cells by inhibiting STAT3

F Hu, Y Zhao, Y Yu, J Fang, R Cui, Z Liu, X Guo, Q Xu - Cancer Letters, 2017

… 3MA, 3-methyladenine; Atg14, autophagy-related 14; CCK-8, Cell Counting Kit-8; DAPI,
4',6-diamidino-2-phenylindole dihydrochloride; IL-6 … Shanghai, China) for 24h after seeding. 2.2.
Cell viability assay Cell viability was evaluated with Cell Counting Kit (CCK)-8 (Dojindo

[HTML] Decreased long non-coding RNA MTM contributes to gastric cancer cell migration and invasion via modulating MT1F

Z Lin, S Lai, X He, W Zhuo, L Wang, J Si, S Chen - Oncotarget, 2017

… After 6 h of culture (day 0), as well as at 24h (day 1), 48h (day 2), 72h (day 3) and 96h (day
4), cell proliferation was measured using Cell counting kit-8 (CCK-8) assays (Dojindo, Japan)
according to the manufacturer's protocols. Colony formation assay …

ZnO nanostructures enhance the osteogenic capacity of SaOS-2 cells on acid-etched pure Ti

R Zhang, Q Huang, X Liu, X Yang, H Yan, Z Xiong… - Materials Letters, 2017

… Cell proliferation was tested by Cell Counting Kit-8 (CCK-8, Dojindo, Japan) at day 1, day 4 and
day 7. The ALP activity of each group at day 7 was measured by ALP kit (Jiancheng
bio-engineering research institute of Nanjing, China). 3. Results and discussion …

Combined Erlotinib and PF-03084014 treatment contributes to synthetic lethality in head and neck squamous cell carcinoma

Y Zheng, Z Wang, X Ding, Y Dong, W Zhang, Y Zhong… - Cell Proliferation, 2017

… 2.8 | Cell counting kit- 8 (CCK- 8) experiments Cells were seeded in 96- well
microplates at a density of 2 × 103 cells per well. Cells were incubated in new medium
containing 10% CCK- 8 reac- tion solution (DOJINDO, Japan) …

MicroRNA-222-3p associated with Helicobacter pylori targets HIPK2 to promote cell proliferation, invasion and inhibits apoptosis in gastric cancer

X Tan, H Tang, J Bi, N Li, Y Jia - Journal of Cellular Biochemistry, 2017

… SGC-7901 cells were seeded into 96-well plates, treated as indicated, and incubated with the
cell proliferation reagent Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Kumamoto, Japan)
(10µL of CCK-8) for 2h. Absorbance was measured at 450nm on …

Silica nanoparticle releases SIRT6-induced epigenetic silencing of follistatin

L Zhang, B Han, J Xiang, K Liu, H Dong, X Gao - The International Journal of …, 2017

… 2.11. CCK8-based viability assay. The effect of SiO 2 NP on cell viability was
assessed by Cell Counting Kit-8 (CCK8) assay (Dojindo Laboratory, Japan). Cells
were then treated with SiO 2 NPs for another 12 h. 10 µl of CCK8 …

[HTML] The Protective Effects of ?-Opioid Receptor Stimulation in Hypoxic Pulmonary Hypertension Involve Inhibition of Autophagy Through the AMPK-MTOR Pathway

Y Zhou, Y Wang, X Wang, X Tian, S Zhang, F Yang… - Cellular Physiology and …, 2017

… AICAR and rapamycin were obtained from Selleck Chemicals. The Cell Counting Kit-8
(CCK-8) was obtained from Dojindo Laboratories (Kumamoto, Japan). The Protein Quantitation
Kit and chemiluminescence kits were purchased from the Pierce Company. Animals …

[HTML] Salidroside attenuates hypoxia-induced pulmonary arterial smooth muscle cell proliferation and apoptosis resistance by upregulating autophagy through the AMPK …

D Gui, Z Cui, L Zhang, C Yu, D Yao, M Xu, M Chen… - BMC Pulmonary Medicine, 2017

… The rabbit antibody against AMPK was obtained from Abcam (Cambridge, UK). Cell counting
kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). The in-situ Cell
Death Detection Kit was purchased from Roche Diagnostics (Penzberg, Germany) …

Enhanced Gene Transfection Efficiency by Use of Peptide Vectors Containing Laminin Receptor Targeting Sequence YIGSR

Z Meng, Z Kang, C Sun, S Yang, B Zhao, S Feng… - Nanoscale, 2017

… Bradford protein assay kit was purchased from Beijing Solarbio Science &Technology Co. Ltd.
(Beijing, China). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular
Technologies, Inc. DNase I was obtained from BBI Life Sciences Corporation …

[HTML] Effect of siRNA-silencing of SALL2 gene on growth, migration and invasion of human ovarian carcinoma A2780 cells

F Miao, X Zhang, Y Cao, Y Wang, X Zhang - BMC Cancer, 2017

… The cells were incubated at 37 °C under 5% CO 2 . They were subsequently incubated for an
additional 2 h with 10 µl CCK-8 (Dojindo, Kumamoto, Japan) for 24, 48, and 72 h. The absorbance
at 450 nm was measured using a microplate reader (Tecan M200 PRO, Switzerland …

The atypical protein kinase RIOK3 contributes to glioma cell proliferation/survival, migration/invasion and the AKT/mTOR signaling pathway

T Zhang, D Ji, P Wang, L Dong, L Jin, H Shi, X Liu… - Cancer Letters, 2017

… The surviving cells were used in the subsequent experiments. 2.11. Cell growth assays. Cell
viability was measured with a Cell Counting Kit-8 (CCK-8, Dojindo, Japan). EdU
(5-ethynyl-20-deoxyuridine) assay was performed with a commercial kit (Ribobio, Guangzhou …

[HTML] Taurine Protects Mouse Spermatocytes from Ionizing Radiation-Induced Damage Through Activation of Nrf2/HO-1 Signaling

W Yang, J Huang, B Xiao, Y Liu, Y Zhu, F Wang, S Sun - Cellular Physiology and …, 2017

… Cell viability assay. Cell viability was determined by Cell counting kit-8 (CCK-8, Dojindo,
Japan). According to the manufacturer's instructions, GC-2 cells (5 × 10 3 cells/well) were
seeded into 96-well culture plate in 100µl of cell culture medium …

Asperosaponin VI promotes angiogenesis and accelerates wound healing in rats via up-regulating HIF-1a/VEGF signaling

C Wang, Y Lou, M Tong, L Zhang, Z Zhang, Y Feng… - Acta pharmacologica Sinica, 2017

… Assessment of cellular viability and proliferation. Cell viability was evaluated by
Cell Counting Kit-8 assays (CCK-8; Dojindo Co, Japan) according to the
manufacturer's instructions. In brief, HUVECs were seeded on 96-well …

[HTML] Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process.

L Wang, G Li, L Ren, X Kong, Y Wang, X Han, W Jiang… - International Journal of …, 2017

… Cell viability test. Cell Counting Kit-8 (CCK-8) assay was performed according to the
manufacturer's instructions (Dojindo, Kumamoto, Japan) to evaluate the viability of
hBMSCs under the influence of 317L-Cu SS. First, the small …

[HTML] Biocompatible 5-Aminolevulinic Acid/Au Nanoparticle-Loaded Ethosomal Vesicles for In Vitro Transdermal Synergistic Photodynamic/Photothermal Therapy of …

Z Zhang, Y Chen, J Ding, C Zhang, A Zhang, D He… - Nanoscale Research Letters, 2017

… respectively. After 12 h, the cell viability was measured using a cell counting kit-8
(CCK-8, Dojindo, Japan) following the manufacturer's instructions. PDT/PTT Procedure.
HSF were seeded in 12-well plates at 4 × 10 4 cells/well …

[PDF] Ginsenoside Rg1 protects human renal tubular epithelial cells from lipopolysaccharide-induced apoptosis and inflammation damage

XJ Ni, ZQ Xu, H Jin, SL Zheng, Y Cai, JJ Wang

… CCK-8 assay The viability of HK-2 cells was assessed by using a Cell Counting
Kit-8 (CCK-8, Dojindo Molecular Technologies, USA). Briefly, cells were seeded
on a 96-well plate with a density of 5 x 103 cells/well, and then …

Extracts of Cordyceps sinensis inhibit breast cancer cell metastasis via down-regulation of metastasis-related cytokines expression

H Cai, J Li, B Gu, Y Xiao, R Chen, X Liu, X Xie, L Cao - Journal of …, 2017

… WECS, water extracts of Cordyceps sinensis; MMP-9, matrix metalloproteinase-9; CCK8, cell
counting kit-8; ELISA, enzyme-linked immunosorbent assay; … 0.40 mg/ml) of WECS for additional
72 h. The cell viability was measured by CCK8 assay (Dojindo Molecular Technologies …

Nitric oxide promotes epidermal stem cell proliferation via FOXG1-c-Myc signalling

R Zhan, F Wang, Y Wu, Y Wang, W Qian, M Liu, T Liu… - Nitric Oxide, 2017

… For the CCK-8 (Dojindo, Kumamoto, Japan) test, the cells were mixed with 10 ml of CCK-8 solution
per well and were incubated for an … In the cell proliferation assay, the cell counting kit-8
(CCK-8) test (http://www.nature.com/onc/journal/v33/n22/full/onc2013250a.htmlFig

[HTML] ROS-induced cleavage of NHLRC2 by caspase-8 leads to apoptotic cell death in the HCT116 human colon cancer cell line

K Nishi, Y Iwaihara, T Tsunoda, K Doi, T Sakata… - Cell Death & Disease, 2017

Article.

Fatty acid oxidation contributes to IL-1ß secretion in M2 macrophages and promotes macrophage-mediated tumor cell migration

Q Zhang, H Wang, C Mao, M Sun, G Dominah, L Chen… - Molecular Immunology, 2018

… 96-well plate and were cultured in normal or conditioned medium (1:1 diluted with fresh complete
medium) for 48 h. Cell proliferation was then detected using Cell Counting Kit-8 (Dojindo
Molecular Techonlogies, Rockville, MD, USA) according to the manufacture's instructions …

MiR-564 is down-regulated in osteosarcoma and inhibits the proliferation of osteosarcoma cells via targeting Akt

N Ru, F Zhang, J Liang, Y Du, W Wu, F Wang, X Liu - Gene, 2017

… 2.5. Cell viability assay. OS cells transfected with the indicate miRNA were seeded in the 96-well
plate with a density of 5 × 10 3 cells per well. The cell viability was determined by adding 10 µl
Cell Counting Kit-8 (CCK-8) reagent (Dojindo, Rockville, MD) into the culture medium …

[HTML] ESTABLISHMENT AND CHARACTERIZATION OF CELL LINE OF UNDIFFERENTIATED PLEOMORPHIC SARCOMA

Y TAKAI, R OYAMA, K Fusako, M SAKUMOTO… - TISSUE CULTURE …, 2017

… At 24, 48, 72, 96, and 120 h, 10 µL of Cell Counting Kit 8 (CCK-8) reagent (Dojindo Molecular
Technologies, Inc., Kumamoto, Japan) was added to each well and incubated for 2 h. Absorbance
values at a wavelength of 450 nm were recorded using a microplate reader (Bio-Rad …

Long non-coding RNA XLOC_008466 acts as an oncogenic molecular in cervical cancer tumorigenesis

F Guo, YZ Chen, L Li, C Chen, JH Jin, J Yang, JJ Chen… - Biomedicine & …, 2018

… For cervical cancer cells proliferation, cell-counting kit-8 (CCK-8) assay and colony formation
assay were performed. For CCK-8 assay, briefly, HT-3 and HeLa cells (5 × 10 3 cells/well) were
seeded into 96-well plates and cultured with CCK-8 reagent (Dojindo, Japan) at 37 °C …

[HTML] Decreased expression of interferon-induced protein 2 (IFIT2) by Wnt/ß-catenin signaling confers anti-apoptotic properties to colorectal cancer cells

T Ohsugi, K Yamaguchi, C Zhu, T Ikenoue, Y Furukawa - Oncotarget, 2017

… Cell proliferation assay. Cell proliferation assay was carried out by water soluble
tetrazolium salts (WST)-based colorimetric method using Cell-counting kit-8 according
to the supplier's protocol (Dojindo, Kumamoto, Japan). Absorbance …

IGFBP7 regulates the osteogenic differentiation of bone marrow–derived mesenchymal stem cells via Wnt/ß-catenin signaling pathway

W Zhang, E Chen, M Chen, C Ye, Y Qi, Q Ding, H Li… - The FASEB Journal, 2017

… After adherence for 24 h, cells were treated with 10% cell counting kit-8 (CCK-8) (Dojindo,
Kumamoto, Japan) in 150 ml a-MEM without FBS for 3 h at 37°C. Absorbance was measured
at 450 nm, which indirectly represents the cell proliferation rate, on a microplate reader …

STMN1, a prognostic predictor of esophageal squamous cell carcinoma, is a marker of the activation of the PI3K pathway

W Jiang, S Huang, L Song, Z Wang - Oncology Reports, 2018

… Cell proliferation assay. In the cell proliferation assay using a Cell Counting Kit-8 (CCK-8;
Dojindo Laboratories, Kymamoto, Japan), viable cells were seeded into 96-well tissue culture
plates such that there were 3,000 cells in a final volume of 100 µl/well …

Docetaxel-mediated autophagy promotes chemoresistance in castration-resistant prostate cancer cells by inhibiting STAT3

F Hu, Y Zhao, Y Yu, J Fang, R Cui, Z Liu, X Guo, Q Xu - Cancer Letters, 2017

… 3MA, 3-methyladenine; Atg14, autophagy-related 14; CCK-8, Cell Counting Kit-8; DAPI,
4',6-diamidino-2-phenylindole dihydrochloride; IL-6 … Shanghai, China) for 24h after seeding. 2.2.
Cell viability assay Cell viability was evaluated with Cell Counting Kit (CCK)-8 (Dojindo

[HTML] Decreased long non-coding RNA MTM contributes to gastric cancer cell migration and invasion via modulating MT1F

Z Lin, S Lai, X He, W Zhuo, L Wang, J Si, S Chen - Oncotarget, 2017

… After 6 h of culture (day 0), as well as at 24h (day 1), 48h (day 2), 72h (day 3) and 96h (day
4), cell proliferation was measured using Cell counting kit-8 (CCK-8) assays (Dojindo, Japan)
according to the manufacturer's protocols. Colony formation assay …

ZnO nanostructures enhance the osteogenic capacity of SaOS-2 cells on acid-etched pure Ti

R Zhang, Q Huang, X Liu, X Yang, H Yan, Z Xiong… - Materials Letters, 2017

… Cell proliferation was tested by Cell Counting Kit-8 (CCK-8, Dojindo, Japan) at day 1, day 4 and
day 7. The ALP activity of each group at day 7 was measured by ALP kit (Jiancheng
bio-engineering research institute of Nanjing, China). 3. Results and discussion …

Combined Erlotinib and PF-03084014 treatment contributes to synthetic lethality in head and neck squamous cell carcinoma

Y Zheng, Z Wang, X Ding, Y Dong, W Zhang, Y Zhong… - Cell Proliferation, 2017

… 2.8 | Cell counting kit- 8 (CCK- 8) experiments Cells were seeded in 96- well
microplates at a density of 2 × 103 cells per well. Cells were incubated in new medium
containing 10% CCK- 8 reac- tion solution (DOJINDO, Japan) …

MicroRNA-222-3p associated with Helicobacter pylori targets HIPK2 to promote cell proliferation, invasion and inhibits apoptosis in gastric cancer

X Tan, H Tang, J Bi, N Li, Y Jia - Journal of Cellular Biochemistry, 2017

… SGC-7901 cells were seeded into 96-well plates, treated as indicated, and incubated with the
cell proliferation reagent Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Kumamoto, Japan)
(10µL of CCK-8) for 2h. Absorbance was measured at 450nm on …

Silica nanoparticle releases SIRT6-induced epigenetic silencing of follistatin

L Zhang, B Han, J Xiang, K Liu, H Dong, X Gao - The International Journal of …, 2017

… 2.11. CCK8-based viability assay. The effect of SiO 2 NP on cell viability was
assessed by Cell Counting Kit-8 (CCK8) assay (Dojindo Laboratory, Japan). Cells
were then treated with SiO 2 NPs for another 12 h. 10 µl of CCK8 …

[HTML] The Protective Effects of ?-Opioid Receptor Stimulation in Hypoxic Pulmonary Hypertension Involve Inhibition of Autophagy Through the AMPK-MTOR Pathway

Y Zhou, Y Wang, X Wang, X Tian, S Zhang, F Yang… - Cellular Physiology and …, 2017

… AICAR and rapamycin were obtained from Selleck Chemicals. The Cell Counting Kit-8
(CCK-8) was obtained from Dojindo Laboratories (Kumamoto, Japan). The Protein Quantitation
Kit and chemiluminescence kits were purchased from the Pierce Company. Animals …

[HTML] Salidroside attenuates hypoxia-induced pulmonary arterial smooth muscle cell proliferation and apoptosis resistance by upregulating autophagy through the AMPK …

D Gui, Z Cui, L Zhang, C Yu, D Yao, M Xu, M Chen… - BMC Pulmonary Medicine, 2017

… The rabbit antibody against AMPK was obtained from Abcam (Cambridge, UK). Cell counting
kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). The in-situ Cell
Death Detection Kit was purchased from Roche Diagnostics (Penzberg, Germany) …

Enhanced Gene Transfection Efficiency by Use of Peptide Vectors Containing Laminin Receptor Targeting Sequence YIGSR

Z Meng, Z Kang, C Sun, S Yang, B Zhao, S Feng… - Nanoscale, 2017

… Bradford protein assay kit was purchased from Beijing Solarbio Science &Technology Co. Ltd.
(Beijing, China). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular
Technologies, Inc. DNase I was obtained from BBI Life Sciences Corporation …

[HTML] Effect of siRNA-silencing of SALL2 gene on growth, migration and invasion of human ovarian carcinoma A2780 cells

F Miao, X Zhang, Y Cao, Y Wang, X Zhang - BMC Cancer, 2017

… The cells were incubated at 37 °C under 5% CO 2 . They were subsequently incubated for an
additional 2 h with 10 µl CCK-8 (Dojindo, Kumamoto, Japan) for 24, 48, and 72 h. The absorbance
at 450 nm was measured using a microplate reader (Tecan M200 PRO, Switzerland …

The atypical protein kinase RIOK3 contributes to glioma cell proliferation/survival, migration/invasion and the AKT/mTOR signaling pathway

T Zhang, D Ji, P Wang, L Dong, L Jin, H Shi, X Liu… - Cancer Letters, 2017

… The surviving cells were used in the subsequent experiments. 2.11. Cell growth assays. Cell
viability was measured with a Cell Counting Kit-8 (CCK-8, Dojindo, Japan). EdU
(5-ethynyl-20-deoxyuridine) assay was performed with a commercial kit (Ribobio, Guangzhou …

[HTML] Taurine Protects Mouse Spermatocytes from Ionizing Radiation-Induced Damage Through Activation of Nrf2/HO-1 Signaling

W Yang, J Huang, B Xiao, Y Liu, Y Zhu, F Wang, S Sun - Cellular Physiology and …, 2017

… Cell viability assay. Cell viability was determined by Cell counting kit-8 (CCK-8, Dojindo,
Japan). According to the manufacturer's instructions, GC-2 cells (5 × 10 3 cells/well) were
seeded into 96-well culture plate in 100µl of cell culture medium …

Asperosaponin VI promotes angiogenesis and accelerates wound healing in rats via up-regulating HIF-1a/VEGF signaling

C Wang, Y Lou, M Tong, L Zhang, Z Zhang, Y Feng… - Acta pharmacologica Sinica, 2017

… Assessment of cellular viability and proliferation. Cell viability was evaluated by
Cell Counting Kit-8 assays (CCK-8; Dojindo Co, Japan) according to the
manufacturer's instructions. In brief, HUVECs were seeded on 96-well …

[HTML] Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process.

L Wang, G Li, L Ren, X Kong, Y Wang, X Han, W Jiang… - International Journal of …, 2017

… Cell viability test. Cell Counting Kit-8 (CCK-8) assay was performed according to the
manufacturer's instructions (Dojindo, Kumamoto, Japan) to evaluate the viability of
hBMSCs under the influence of 317L-Cu SS. First, the small …

[PDF] MicroRNA-376a inhibits cell proliferation and invasion in glioblastoma multiforme by directly targeting specificity protein 1

Y Li, Y Wu, Z Sun, R Wang, D Ma - Molecular Medicine Reports, 2018

Cell Counting Kit-8 (CCK-8) proliferation assay. The prolif- erative abilities of GBM
U251 cells were determined using CCK-8 proliferation assays (Dojindo Molecular
Technologies, Inc., Kumamoto, Japan). Transfected cells …

[PDF] Paeoniflorin blocks the proliferation of vascular smooth muscle cells induced by platelet-derived growth factor-BB through ROS mediated ERK1/2 and p38 signaling …

X Fan, J Wu, H Yang, L Yan, S Wang - Molecular Medicine Reports, 2018

… (Rock Hill, NJ, USA). Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Molecular
Technologies, Inc. (Kumamoto, Japan). A cell proliferation ELISA, BrdU (colo- rimetric) kit was
purchased from Roche (11647229001; Roche Diagnostics, Basel, Switzerland) …

Thioredoxin-interacting protein deficiency ameliorates diabetic retinal angiogenesis

J Duan, C Du, Y Shi, D Liu, J Ma - The International Journal of Biochemistry & Cell …, 2017

… Mouse VEGF ELISA Kit was purchased from Abcam (Cambridge, UK). The cell counting
kit-8(CCK-8) was obtained from Dojindo (Kumamoto, Japan). N-Acetyl-L-cysteine (NAC),
D-glucose and mannitol were purchased from Sigma (St. Louis, MO, USA). 2.2 …

Fabrication of biomimetic resorption lacunae-like structure on titanium surface and its osteoblast responses

F Huo, W Guo, H Wu, Y Wang, G He, L Xie, W Tian - Applied Surface Science, 2017

… Subsequently the samples with immobilized cells were observed by FE-SEM. 2.6. Cell proliferation
and differentiation. Cell Counting Kit-8 (CCK-8; Dojindo Molecular Technologies, Tokyo, Japan)
was used to examine viability of MG63 cells after culturing for 1 to 5 days …

microRNA-625 targets Yes-associated protein 1 to suppress cell proliferation and invasion of osteosarcoma

Z Luo, G Wu, D Zhang, J Liu, R Ran - Molecular Medicine Reports, 2018

Cell Counting Kit-8 (CCK-8) and Transwell inva- sion assays were conducted at 24 and
48 h post transfection … CCK-8 assay. Cell proliferation was determined using the CCK-8
assay (Dojindo Molecular Technologies, Inc., Rockville, MD, USA) …

Strontium incorporation improves the bone-forming ability of scaffolds derived from porcine bone

D Cheng, Q Liang, Y Li, J Fan, G Wang, H Pan, C Ruan - Colloids and Surfaces B …, 2017

… Proliferation of MSCs on scaffolds after 1, 3, 5, and 7 days of culture was evaluated
by Cell Counting Kit-8 (CCK-8, Dojindo Laboratories, Japan) following the protocol
provided by the manufacturer. The absorbance at 450 nm …

KLF4 protects brain microvascular endothelial cells from ischemic stroke induced apoptosis by transcriptionally activating MALAT1

H Yang, X Xi, B Zhao, Z Su, Z Wang - Biochemical and Biophysical Research …, 2017

… 2.7. Assessment of cell viability and caspase-3 activity. Cell viability was quantified
using WST-8 assay with Cell Counting Kit-8 (CCK-8, Dojindo, Tokyo, Japan) according
to manufacturer's instruction. Caspase-3 activity in b.End3 …

[HTML] Proteomic Analysis Reveals Grb2 as a Key Regulator of Periodic Mechanical Stress Transduction in Chondrocytes

S Xu, Z Li, Z Wang, C Zhai, W Liang, C Zhu, W Fan - Cellular Physiology and …, 2017

… CCK-8 assay. Cell proliferation was determined using CCK-8 solution according to the
manufacturer's instructions (Dojindo Lab., Kumamoto, Japan) … (C, D) Direct cell counting and
cell counting kit-8 (CCK-8) assay of chondrocytes. Data are represented as mean ± SD …

[HTML] Lysophosphatidylcholine acyltransferase 4 is involved in chondrogenic differentiation of ATDC5 cells

S Tabe, H Hikiji, W Ariyoshi, T Hashidate-Yoshida… - Scientific Reports, 2017

Article.

[HTML] Apatinib Inhibits Angiogenesis Via Suppressing Akt/GSK3ß/ANG Signaling Pathway in Anaplastic Thyroid Cancer

Z Jin, X Cheng, H Feng, J Kuang, W Yang, C Peng… - Cellular Physiology and …, 2017

… Louis, MO, USA). CCK-8 Assays of Cell Viability and Colony Formation Assay. The cytotoxicity
of Apatinib was estimated using the CCK-8 assay (Cell Counting Kit-8, Dojindo, Kumamoto, Japan).
Cells were plated in 96-well plates at 3, 000 cells per well 24 h before treatment …

[HTML] Collagen I promotes hepatocellular carcinoma cell proliferation by regulating integrin ß1/FAK signaling pathway in nonalcoholic fatty liver

X Zheng, W Liu, J Xiang, P Liu, M Ke, B Wang, R Wu… - Oncotarget, 2017

… CCK-8 assay. The viability of HCC cells cultured on different ECM or adding EGF were
quantitatively determined by the 2-(2-methoxy-4-nitriphenyl)-3-(4-nitrophenyl)-5-.
(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt (CCK-8) assay (Cell Counting Kit-8, Dojindo) …

[HTML] MicroRNA-936 induces cell cycle arrest and inhibits glioma cell proliferation by targeting CKS1

D Wang, T Zhi, X Xu, Z Bao, L Fan, Z Li, J Ji, N Liu - American Journal of Cancer …, 2017

Cell counting kit-8 (CCK-8) assay. U87 and U251 cells were seeded in 96-well plates
at a density of 2000 cells per well. And the CCK-8 assay (Dojindo, Japan) were
performing when the cells were cultured at 24, 48, 72 and 96 hs …

[HTML] Oncogene TUBA1C promotes migration and proliferation in hepatocellular carcinoma and predicts a poor prognosis

J Wang, W Chen, W Wei, J Lou - Oncotarget, 2017

… Each step was performed three times. For the cell proliferation assay, HCC cells were seeded
into a 96-well plates (3000/well) and use Cell Counting Kit-8 (Dojindo Laboratories, JA) per 24
hours, following the manufacturer provided protocols to test the proliferation rate …

[HTML] Long non-coding RNA UCA1 indicates an unfavorable prognosis and promotes tumorigenesis via regulating AKT/GSK-3ß signaling pathway in cholangiocarcinoma

Y Xu, Y Yao, K Leng, Z Li, W Qin, X Zhong, P Kang… - Oncotarget, 2017

… Afterwards, 10 µL of cell counting kit-8 (CCK-8) reagent (Dojindo, Kumamoto, Japan) was added
into each well before incubated at 37°C for 2 h. A microplate reader (Tecan, Männedorf,
Switzerland) was used to detect absorbance value at 450 nm wavelength …

[HTML] Diphlorethohydroxycarmalol from Ishige okamurae Suppresses Osteoclast Differentiation by Downregulating the NF-?B Signaling Pathway

HJ Ihn, JA Kim, HS Cho, HI Shin, GY Kim, YH Choi… - International Journal of …, 2017

Marine algae possess a variety of beneficial effects on human health. In this study, we investigated
whether diphlorethohydroxycarmalol (DPHC), isolated from Ishige okamurae, a brown alga,
suppresses receptor activator of nuclear factor-?B ligand (RANKL)-induced osteoclast …

Low-level ultrahigh-frequency and ultrashort-pulse blue laser irradiation enhances osteoblast extracellular calcification by upregulating proliferation and …

R Mikami, K Mizutani, A Aoki, Y Tamura, K Aoki… - Lasers in Surgery and …, 2017

… 2 MIKAMI ET AL. Page 3. Kit-8; Dojindo, Kumamoto, Japan) assay was employed.
This assay enables sensitive colorimetric assays for determination of cell viability
in cell proliferation and cytotoxicity assays. The water-soluble …

[HTML] Autophagy inhibition sensitizes LY3023414-induced anti-glioma cell activity in vitro and in vivo

L Zheng, H Li, Y Mo, G Qi, B Liu, J Zhao - Oncotarget, 2017

… Cells were initially seeded onto 96-well plates at 1 ×10 4 cells/well. After treatment, cell survival
was measured by the Cell Counting Kit-8 (CCK-8) (Dojindo, Japan) assay. The optical density
(OD) value of CCK-8 was recorded. Colony formation assay …

[HTML] Dual inhibition of BRD4 and PI3K-AKT by SF2523 suppresses human renal cell carcinoma cell growth

H Zhu, JH Mao, Y Wang, DH Gu, XD Pan, Y Shan… - Oncotarget, 2017

… Experiments were according to the principles of Declaration of Helsinki. CCK-8 assay. Cell
Counting Kit-8 (CCK-8; Dojindo, Kumamoto, Japan) was applied to detect cell survival. The optical
density (OD) of CCK-8 was measured at 450 nm. Trypan blue staining assay …

Apatinib inhibits migration and invasion as well as PD-L1 expression in osteosarcoma by targeting STAT3

B Zheng, T Ren, Y Huang, W Guo - Biochemical and Biophysical Research …, 2017

… well plates. The cells were treated with Apatinib at different concentration. Cell
proliferation was analyzed using Cell Counting Kit 8 (Dojindo, Kumamoto, Japan)
according to the manufacturer's protocol. 2.4. Transwell assay …

Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells

L Shang, T Chen, Y Deng, Y Huang, Y Huang, J Xian… - PLoS One, 2017

… Cell proliferation assays were performed using the cell counting kit-8 (CCK-8) (Dojindo,
Kumamoto, Japan) according to the manufacturer's instructions. Two groups of cells
were seeded into 96-well plates at a density of 1 × 103 …

Self-assembly regulated anticancer activity of platinum coordinated selenomethionine

T Li, W Xiang, F Li, H Xu - Biomaterials, 2017

… After 24 h, cytotoxicity was quantified by adding 10 µL cell counting kit-8 (Dojindo, Japan)
reagent to each well. The cells were incubated at 310 K for 1 h. Then, the absorbance at 450
nm was read on a microplate reader (Themo Multiscan MK3, USA) …

[HTML] DUSP4 promotes doxorubicin resistance in gastric cancer through epithelial-mesenchymal transition

X Kang, M Li, H Zhu, X Lu, J Miao, S Du, X Xia, W Guan - Oncotarget, 2017

Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay were
used to measure cell viability and proliferation in gastric cancer cells treated with doxorubicin …
Cell viability was assessed using the CCK-8 assay (Dojindo, Kumamoto, Japan) …

Long non-coding RNA SOX21-AS1 sponges miR-145 to promote the tumorigenesis of colorectal cancer by targeting MYO6

AW Wei, LF Li - Biomedicine & Pharmacotherapy, 2017

… With culture for 24 h, the proliferation of CRC cells was measured using Cell Counting
Kit-8 (Dojindo, Tokyo, Japan) according to the manufacturer's illustration. The
absorbance of CRC cells was measured at 450 nm at pointed time …

[PDF] MicroRNA-582-5p suppressed gastric cancer cell proliferation via targeting AKT3

Y Jin, LP TAO, SC YAO, QK HUANG, ZF CHEN… - European Review for …, 2017

Cell Counting kit-8 Assay CCK8 (Dojindo, Kumamoto, Japan) assay was applied to investigate
the cell proliferation. The cells were planted in a density of 1*103 cells with 100 µL medium,
and then were cultured for 24, 48, 72, 96 h after transfection …

[HTML] Long non-coding RNA GAS5 suppresses pancreatic cancer metastasis through modulating miR-32-5p/PTEN axis

ZQ Gao, J Wang, DH Chen, XS Ma, Y Wu, Z Tang… - Cell & Bioscience, 2017

… The relative cell viability was evaluated by Cell Counting Kit-8 assay, which is a widely used
method for analysis of cell viability. In brief, PANC-1 and BxPC-3 cells were cultured in 96-well
plates with 6 × 10 3 cells/well. CCK-8 solution (10 µl, Dojindo) was added in each well …

[HTML] FOXA1 inhibits hepatocellular carcinoma progression by suppressing PIK3R1 expression in male patients

S He, J Zhang, W Zhang, F Chen, R Luo - Journal of Experimental & Clinical Cancer …, 2017

… For the Cell Counting Kit-8 (CCK-8) assay, transfected HepG2 and Hep3B cells were seeded
in 96-well plates at a density of 10 3 … Subsequently, 100 µL of complete medium supplemented
with 10 µL of CCK-8 solution (Dojindo, Kumamoto, Japan) was added to each well, the …

[HTML] Can zinc protect cells from the cytotoxic effects of cobalt ions and nanoparticles derived from metal-on-metal joint arthroplasties?

Y Liu, H Zhu, H Hong, W Wang, F Liu - Bone and Joint Research, 2017

Skip to main content …

[PDF] Promoter methylation of Wnt/ß-Catenin signal inhibitor TMEM88 is associated with unfavorable prognosis of nonsmall cell lung cancer

R Ma, N Feng, X Yu, H Lin, X Zhang, O Shi, H Zhang… - Cancer Biology & Medicine, 2017

… Cell proliferation was analyzed by using Cell Counting Kit-8 (Dojindo, Cat no. CK04, Japan).
After DAC treatment for 0 h, 24 h, and 48 h, the cells were treated with 10% CCK-8 in new
media for 2 h at 37 °C and the absorbance at 450 nm was measured …

[HTML] MicroRNA-218 inhibits proliferation and invasion in ovarian cancer by targeting Runx2

N Li, L Wang, G Tan, Z Guo, L Liu, M Yang, J He - Oncotarget, 2017

… CCK8 assay. Ovarian cancer cell proliferation was evaluated by means of the Cell
Counting Kit-8 assay (CCK-8; Dojindo, Kumamoto, Japan) in accordance to
manufacturer's instructions. The optical density (OD) was measured …

[HTML] LncRNA-UCA1 enhances MMP-13 expression by inhibiting miR-204-5p in human chondrocytes

G Wang, X Bu, Y Zhang, X Zhao, Y Kong, L Ma, S Niu… - Oncotarget, 2017

… Cell proliferation was measured by Cell Counting Kit 8 (CCK-8, Dojindo, Japan) following
to the manufacturer's information. The end product was determined spectrophotometrically
at the wavelength of 450 nm. Luciferase reporter assay …

[HTML] MicroRNA-21 inhibits mitochondria-mediated apoptosis in keloid

H Wu, J Wang, H Ma, Z Xiao, X Dong - Oncotarget, 2017

… Upregulated miR-21 promotes cell proliferation of human KF. The effect of miR-21 on proliferation
of keloid fibroblasts was detected by cell counting kit-8 (CCK8) assay … CCK8 assay (Dojindo,
China) was performed to assess the role of miRNA-21 in keloid fibroblasts …

[HTML] CLCA4 inhibits bladder cancer cell proliferation, migration, and invasion by suppressing the PI3K/AKT pathway

T Hou, L Zhou, L Wang, G Kazobinka, X Zhang, Z Chen - Oncotarget, 2017

Cell counting kit-8 (CCK-8) assay. Cell viability was quantified by using the CCK-8
kit (DOJINDO Laboratories, Japan) as previously described [15]. Cells were plated
on 60 mm plates (0.5×10 3 cells/plate) and cultured for 10 days …

[HTML] MicroRNA-30c-5p ameliorates hypoxia-reoxygenation-induced tubular epithelial cell injury via HIF1a stabilization by targeting SOCS3

YF Zou, WT Liao, ZJ Fu, Q Zhao, YX Chen, W Zhang - Oncotarget, 2017

… cells were cultured in 96-well plates, and fresh medium was added after the oligo transfection
and H/R. A total of 10 µl of CCK-8 (Cell counting kit-8, Dojindo, Japan) solution was added into
the wells and incubated at 37°C for 1-4 h according to the manufacturer's instructions …

[PDF] Metabolic reprogramming induced by inhibition of SLC2A1 suppresses tumor progression in lung adenocarcinoma

Y Wang, S Shi, Y Ding, Z Wang, S Liu, J Yang, T Xu - Int J Clin Exp Pathol, 2017

… Cell viability assay For cell viability assay, cells were seeded into a 96-well plate at 3 ×
103 cells per well with 100 µl culture medium and cultured at 37°C. The cell viability
was quantified by Cell Counting Kit-8 (CCK-8, Dojindo, Japan) …

Ginsenoside Rg3 sensitizes hypoxic lung cancer cells to cisplatin via blocking of NF-?B mediated epithelial–mesenchymal transition and stemness

J Wang, L Tian, MN Khan, L Zhang, Q Chen, Y Zhao… - Cancer Letters, 2017

… using Image J 1.46r software (NIH, Bethesda, MD, USA). 2.4 Cell viability assay Cell viability
was detected using Cell Counting Kit-8 (CCK-8, Dojindo Laboratories, Kumamoto, Japan)
according to the manufacturer's instructions. Briefly, 3,000 Cells were …

[HTML] Alteration of exosomes secreted from renal tubular epithelial cells exposed to high-concentration oxalate

Z He, X Guan, Y Liu, Z Tao, Q Liu, J Wu, Y Deng - Oncotarget, 2017

… 0.5, 1, 2, 4, 5, 8, or 10 mM) for 24, 48, or 72 h. Cell proliferation and cytotoxicity were evaluated
with the Cell Counting Kit-8 (CCK-8 … cells were exposed to different concentrations of Ox (100
µl/well) for 24, 48, or 72 h. Ten microliters of CCK-8 solution (Dojindo, Kamimashiki-gun …

miR-21 Inhibitors Modulate Biological Functions of Gastric Cancer Cells Via PTEN/PI3K/mTOR Pathway

P Wang, Q Guan, D Zhou, Z Yu, Y Song, W Qiu - DNA and Cell Biology, 2017

… Cell proliferation Cell Counting Kit-8 (CCK-8) assay was performed to detect cell proliferation …
At 24, 48, 72, and 96 h after incubation respectively, 10 µL of CCK-8 (Dojindo) was added to
each well, and plates were incu- bated at 37°C for another 1–4 h. The optical density (OD …

[HTML] Kaempferide Prevents Titanium Particle Induced Osteolysis by Suppressing JNK Activation during Osteoclast Formation

Z Jiao, W Xu, J Zheng, P Shen, A Qin, S Zhang, C Yang - Scientific Reports, 2017

Article.

[HTML] PGC-1alpha levels correlate with survival in patients with stage III NSCLC and may define a new biomarker to metabolism-targeted therapy

A Cruz-Bermúdez, RJ Vicente-Blanco… - Scientific Reports, 2017

Article.

[PDF] MicroRNA-520d-5p inhibits human glioma cell proliferation and induces cell cycle arrest by directly targeting PTTG1

T Zhi, K Jiang, X Xu, T Yu, W Wu, E Nie, X Zhou, X Jin… - Am J Transl Res, 2017

… Cell proliferation assay For the cell counting kit-8 (CCK-8) assay, sta- bled transfected
U87-MG and LN229 cells were seeded in 96-well plates, and then cells were cultured for
24, 48, 72 and 96 h before per- forming the CCK-8 assay (Dojindo, Japan) …

[PDF] Antitumor Activity and Mechanism of a Reverse Transcriptase Inhibitor, Dapivirine, in Glioblastoma

W Liu, X Song, S Zhao, M He, H Wang, Z Chen… - Journal of Cancer, 2018

… or controls (DMSO). To determine IC50 concentrations, cells were treated for 48
h, and the viability of cells was determined according to Cell Counting Kit-8
manufactures (Dojindo Laboratories, Japan). Cells were cultured at …

TUG1 knockdown ameliorates atherosclerosis via up-regulating the expression of miR-133a target gene FGF1

L Zhang, H Cheng, Y Yue, S Li, D Zhang, R He - Cardiovascular Pathology, 2017

… assay system (Promega). 2.10. Cell Counting Kit-8 (CCK-8) assay Cell proliferation
was assessed using CCK-8 assay (Dojindo, Kumamoto, Japan) according to the
manufacturer's instruction. Transfected RAW264.7 and MOVAS …

[HTML] Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage

Q Zhang, S Bu, J Sun, M Xu, X Yao, K He, D Lai - Stem Cell Research & Therapy, 2017

… further analysis. Cell counting Kit-8 (CCK-8) viability assay. Cell viability was assessed
using a CCK-8 detection kit (Dojindo Molecular Technologies, Inc., Kumamoto, Japan),
according to the manufacturer's protocol. After cells …

[PDF] Multiple Myeloma-Derived Exosomes Regulate the Functions of Mesenchymal Stem Cells Partially via Modulating miR-21 and miR-146a

Q Cheng, X Li, J Liu, Q Ye, Y Chen, S Tan, J Liu - Stem Cells International, 2017

… 2.4. Cell Proliferation Assay. Proliferation of MSCs was determined by various methods
including MTT assay (Sigma, USA), Cell Counting Kit-8 (CCK-8, Dojindo, Japan)
assay, and direct cell counting. For the MTT assay, MSCs …

The miR-875-5p inhibits SATB2 to promote the invasion of lung cancer cells

J Wang, Y Lu, H Ding, T Gu, C Gong, J Sun, Z Zhang… - Gene, 2017

… 2.9. Cell counting Kit-8 (CCK-8) assay. Proliferations of H157 and A549 NSCLC cells were
determined using a CCK-8 (Dojindo, Kumamoto, Japan). The H157 and A549 NSCLC cells
were seeded in 24-well plates at a density of 5 × 10 5 cells/well …

[HTML] SETD7 is a prognosis predicting factor of breast cancer and regulates redox homeostasis

R Huang, X Li, Y Yu, L Ma, S Liu, X Zong, Q Zheng - Oncotarget, 2017

… Cells were seeded onto 96-well plates with each well containing 4500 cells and 100µL culture
medium. CCK-8 (Cell Counting Kit-8; Dojindo Laboratories, Kyushu, Japan) was employed to
examine the cell viability according to the manufacturer's instructions …

Quercetin inhibits renal cyst growth in vitro and via parenteral injection in a polycystic kidney disease mouse model

Y Zhu, T Teng, H Wang, H Guo, L Du, B Yang, X Yin… - Food & Function, 2017

… and goat anti- rabbit (LI-COR, 926-3221) secondary antibodies were used. A cAMP
enzyme immunoassay kit (Sigma, CA200) and cell counting kit-8 (CCK-8) (Dojindo,
CK04) were used for related research. MDCK cyst model …

Micro RNA-518 inhibits gastric cancer cell growth by inducing apoptosis via targeting MDM2

C Feng, Q Xian, S Liu - Biomedicine & Pharmacotherapy, 2018

… Thereafter, the medium was removed, 10 µL cell counting kit 8 (CCK-8) solution (Dojindo
Laboratories, Kumamoto, Japan) was added into the well and the cells were incubated in an
atmosphere of 5% CO 2 at 37 °C for 4 h. The cell viability was analyzed at 450 nm using a …

Core–Shell Bi2Se3@ mSiO2-PEG as a Multifunctional Drug-Delivery Nanoplatform for Synergistic Thermo-Chemotherapy with Infrared Thermal Imaging of Cancer …

Z Li, Z Li, L Sun, B Du, Y Wang, G Zhao, D Yu, S Yang… - Particle & Particle Systems …, 2017

… HUVEC, 4T1 and Hela cells were incubated with the Bi2Se3@mSiO2-PEG NPs dispersions at
various concentrations for 24 and 48 h, and the cell viability was measured by the Cell Counting
Kit-8 (CCK-8) assay. As shown in Figure 4, the cell viability of all cells remained over …

GSDME mediates caspase-3-dependent pyroptosis in gastric cancer

Y Wang, B Yin, D Li, G Wang, X Han, X Sun - Biochemical and Biophysical Research …, 2017

… 2.2. Cell viability assay. The Cell Counting Kit-8 (CCK-8, Dojindo, Japan) was used
to detect cell viability according to the manufacturer's instructions. Briefly, 1 × 10
4 SGC-7901 or 8 × 10 3 MKN-45 were seeded into 96-well …