Cell Counting Kit-8
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June 2017 Publications~

[PDF] Inhibitory effect of dihydroartemisinin on chondrogenic and hypertrophic differentiation of mesenchymal stem cells

Z Cao, C Liu, Y Bai, C Dou, JM Li, DW Shi, SW Dong… - Am J Transl Res, 2017

... Then, 10 µL Cell Counting Kit-8 (CCK8, Dojindo Laboratories, Japan) reagents were
added to each well and incubated for an additional 4 hours. The absor- bance was
read at the wavelength of 450 nm in an automated plate reader. ...

[PDF] Vasohibin 2 as a potential predictor of aggressive behavior of triple-negative breast cancer

B Wang, L Yang, Q Zhao, L Zhu - Am J Transl Res, 2017

... Cell proliferation assay and cell cycle analysis Cells were seeded in 96-well plates
at 1 × 103 per well. Cell proliferation was evaluated using Cell Counting Kit-8
(CCK-8, Dojindo, USA) ac- cording to the manufacturer's instructions. ...

[PDF] Therapeutic potential of ghrelin and des-acyl ghrelin against chemotherapy-induced cardiotoxicity

M Nonaka, N Kurebayashi, T Murayama, M Sugihara… - Endocrine Journal, 2017

... The changes in cell confluency were analyzed by the IncuCyte ZOOM® live-cell imaging
system (Essen BioScience, Ltd., Japan) and cell viability was deter- mined using Cell
Counting Kit-8 (Dojindo Molecular Technologies, Inc., Japan). ...

[PDF] Downregulated long non-coding RNA TRPM2-AS inhibits cisplatin resistance of non-small cell lung cancer cells via activation of p53-p66shc pathway

LY MA, XW XIE, L Ma, JL PANG, XM XIONG… - European Review for …, 2017

... Cell Counting Kit-8 assay The chemo-sensitivity to cisplatin of both A549/DPP cells
and the paired A549 cells was monitored with Cell Counting Kit-8 assay (CCK8,
Dojindo Molecular Technologies, Inc., Kunamo- to, Japan). ...

[PDF] PDE3A is hypermethylated in cisplatin resistant non-small cell lung cancer cells and is a modulator of chemotherapy response

FM TIAN, CY ZHONG, XN WANG, Y Meng - European Review for Medical and …, 2017

... Page 3. PDE3A downregulation in chemioresistant NSCLC 2637 the Cell Counting
Kit-8 (Dojindo, Kumamoto, Japan) according to manufacturer's instruction. Briefly,
CCK-8 solution was added to each well and the plate was ...

[HTML] Downregulation of Talin-1 expression associates with increased proliferation and migration of vascular smooth muscle cells in aortic dissection

X Wei, Y Sun, Y Wu, J Zhu, B Gao, H Yan, Z Zhao… - BMC Cardiovascular …, 2017

... Cell proliferation assay. Cell proliferation was detected by Cell-counting Kit-8 (Dojindo)
according to the manufacturer's instructions. VSMCs were seeded in 96-well plates
at a density of 5 × 10 3 cells per well. After cell adhesion ...

[HTML] Cellular Uptake and Delivery-Dependent Effects of Tb3+-Doped Hydroxyapatite Nanorods

Y Wei, Y He, X Li, H Chen, X Deng - Molecules, 2017

With the increasing interest in hydroxyapatite (HA) nanostructures for use in biomedicine, the
systematic evaluation of their potential effects on biological systems is becoming critically important.
In this work, we report the in vitro cellular uptake, in vivo tissue distributions and toxicity ...

[HTML] The characteristics of stem cells in human degenerative intervertebral disc

L Liang, X Li, D Li, W Jiang, H Wang, J Chen, Z Sun… - Medicine, 2017

Background: The aim of this study is to identify which possessed the best stem-cell-like characteris.

Upregulated long non-coding RNA LOC90784 promotes cell proliferation and invasion and is associated with poor clinical features in HCC

JH Xu, WH Chang, HW Fu, WQ Shu, T Yuan, P Chen - Biochemical and Biophysical …, 2017

... 2.5. Cell proliferation and colony formation assays. Cell proliferation assays were
performed with the Cell Counting Kit-8 (CCK-8, Dojindo, Japan) according to the
manufacturer's protocol. Approximately 2000 cells/well were ...

MicroRNA-493 suppresses hepatocellular carcinoma tumorigenesis through down-regulation of anthrax toxin receptor 1 (ANTXR1) and R-Spondin 2 (RSPO2)

Y Xu, K Ge, J Lu, J Huang, W Wei, Q Huang - Biomedicine & Pharmacotherapy, 2017

... 2.6. Cell proliferation, colony-formation assays and tumorigenesis in nude mice. In
cell proliferation assay the number of viable cells was measured by Cell Counting
Kit-8(Dojindo, Kumamoto, Japan). Briefly, cells were cultured ...

[HTML] Deregulated expression of Cdc6 as BCR/ABL-dependent survival factor in chronic myeloid leukemia cells

JH Zhang, YL He, R Zhu, W Du, JH Xiao - Tumor Biology, 2017

Chronic myeloid leukemia is characterized by the presence of the reciprocal translocation
t(9;22) and the BCR/ABL oncogene. The BCR/ABL oncogene activates multi.

d-Tocotrienol suppresses tumorigenesis by inducing apoptosis and blocking the COX-2/PGE 2 pathway that stimulates tumor–stromal interactions in colon cancer

S Wada, Y Naito, Y Matsushita, M Nouchi, M Kawai… - Journal of Functional Foods, 2017

... HT29 and Colon 26 cells were treated with d-tocotrienol using the same procedure. After
72-h incubation, cell viability was determined using Cell Counting Kit 8 assay, according
to the manufacturer's instructions (Dojindo, Kumamoto, Japan). ...

Anti-androgenic mechanisms of Bisphenol A involve androgen receptor signaling pathway

H Wang, Z Ding, QM Shi, X Ge, HX Wang, MX Li… - Toxicology, 2017

... Carlsbad, California, USA) before use. The Cell Counting Kit-8 (CCK-8) was obtained
from Dojindo Laboratories (Kumamoto, Japan). The ESCORT V Transfection Reagent
was from Sigma Chemical Co. (St. Louis, MO, USA) and ...

[HTML] The effects of lovastatin on the morphology, cell viability and differentiation of stem cells derived from gingiva

BB Kim, M Kim, JB Park - Biomedical Research, 2017

... Cell viability was analysed on days 2, 7, and 14. Viable cells were identified using a Cell Counting
Kit-8 (CCK-8, Dojindo, Tokyo, Japan) assay. The spectrophotometric absorbance of the samples
was measured using a plate reader (BioTek, Winooski, VT, USA) at 450 nm (n=3). ...

Vitamin D enhances omega-3 polyunsaturated fatty acids-induced apoptosis in breast cancer cells

J Yang, S Zhu, G Lin, C Song, Z He - Cell Biology International, 2017

... PARP, poly-ADP-ribose polymerase; FITC, fluorescein isothiocyanate; PI, propidium iodide;
CCK-8, cell counting kit-8; AKT, protein kinase B; Erk1/2, extracellular ... (DOJINDO, Japan) and
incubated for 4 h at 37?. Cells were subjected to absorbance measurement at 450 nm. ...

Differential effect of p38 and MK2 kinase inhibitors on the inflammatory and toxicity biomarkers in vitro

RK Singh, M Diwan, SG Dastidar, AK Najmi - Human & Experimental Toxicology, 2017

Background: Many inflammatory responses including chemotaxis, production of nitric oxide,
and modulation of pro-inflammatory cytokines in immunological cells ar.

microRNA-205 acts as a tumor suppressor and directly targets YAP1 in glioma

T Ji, X Zhang, W Li - Molecular Medicine Reports, 2017

... Following transfection for 24 h, reverse transcrip- tion-quantitative polymerase chain reaction
(RT-qPCR) and a Cell Counting kit 8 (CCK8) assay were ... Cell proliferation was evaluated by
performing a CCK-8 assay (Dojindo Molecular Technologies, Inc., Kumamoto, Japan). ...

[HTML] Overexpression of long non-coding RNA MEG3 suppresses breast cancer cell proliferation, invasion, and angiogenesis through AKT pathway

C Zhang, M Yu, X Li, Z Zhang, C Han, B Yan - Tumor Biology, 2017

Long non-coding RNA MEG3 has been identified as a tumor suppressor which plays important
roles in tumorigenesis; however, its potential role in breast cancer ha.

[HTML] PTTG3P promotes gastric tumour cell proliferation and invasion and is an indicator of poor prognosis

W Weng, S Ni, Y Wang, M Xu, Q Zhang, Y Yang, Y Wu… - Journal of Cellular and …, 2017

... Stably infected cells were maintained with 2 lg/ml puromycin (Sigma-Aldrich). Cell proliferation
assays Cell proliferation was evaluated using Cell Counting Kit-8 (CCK-8) (Dojindo, Kumamoto,
Japan), an EdU DNA imaging kit (Life Technology) and colony-formation assays. ...

[HTML] MicroRNA-1285-5p influences the proliferation and metastasis of non-small-cell lung carcinoma cells via downregulating CDH1 and Smad4

S Zhou, Z Zhang, P Zheng, W Zhao, N Han - Tumor Biology, 2017

Abnormal expression of microRNAs has been reported to regulate gene expression and cancer
cell growth, invasion, and migration. Recently, upregulation of hsa-mi.

lncRNA HULC promotes the growth of hepatocellular carcinoma cells via stabilizing COX-2 protein

H Xiong, B Li, J He, Y Zeng, Y Zhang, F He - Biochemical and Biophysical Research …, 2017

... Cocktail were purchase from Roche (Basel, Switzerland). Cell Counting Kit-8 (CCK-8)
was from Dojindo (Shanghai, China). SiRNAs for USP22, COX-2 and control siRNA were
synthesized by GenePharma (Shanghai, China). 2.2. Cell culture. ...

[HTML] Mithramycin inhibits epithelial-to-mesenchymal transition and invasion by downregulating SP1 and SNAI1 in salivary adenoid cystic carcinoma

J Li, H Gao, L Meng, L Yin - Tumor Biology, 2017

Mithramycin exhibits certain anticancer effects in glioma, metastatic cerebral carcinoma,
malignant lymphoma, chorionic carcinoma and breast cancer. However, it.

[HTML] Identification of curcumin-inhibited extracellular matrix receptors in non–small cell lung cancer A549 cells by RNA sequencing

H Li, H Wu, H Zhang, Y Li, S Li, Q Hou, S Wu, SY Yang - Tumor Biology, 2017

Curcumin is a potent anti-cancer drug in several types of human cancers. Despite of several
preclinical and clinical studies of curcumin, the precise mechanism.

[HTML] Long non-coding RNA DANCR, a prognostic indicator, promotes cell growth and tumorigenicity in gastric cancer

YP Hao, JH Qiu, DB Zhang, CG Yu - Tumor Biology, 2017

Gastric cancer remains the third leading cause of cancer-related mortality worldwide, and
proliferation of gastric cancer represents the major reason for its po.

Effect of various ratios of co-cultured ATDC5 cells and chondrocytes on the expression of cartilaginous phenotype in microcavitary alginate hydrogel

Y Yao, Y Huang, D Qian, S Zhang, Y Chen, B Bai - Journal of Cellular Biochemistry, 2017

... Cell Counting Kit-8 (CCK-8) assay for cell proliferation Samples were collected from each group
on day 1, 3, 7, 10, 14, 21, and 28, respectively. Each bead was cultured in 330µL test reagent
consisting of 30 µL CCK-8 reagent (Dojindo Laboratories, Kumamoto, Japan) and 300 ...

[HTML] Long non-coding RNA CASC2 regulates cell biological behaviour through the MAPK signalling pathway in hepatocellular carcinoma

Y Gan, N Han, X He, J Yu, M Zhang, Y Zhou, H Liang… - Tumor Biology, 2017

Long non-coding RNAs have previously been demonstrated to play important roles in regulating
human diseases, especially cancer. However, the biological function.

[PDF] miR-200c Accelerates Hepatic Stellate Cell-Induced Liver Fibrosis via Targeting the FOG2/PI3K Pathway

T Ma, X Cai, Z Wang, L Huang, C Wang, S Jiang… - BioMed Research …, 2017

... Lipofectamine 2000 and Trizol were obtained from Invitrogen. Cell Counting Kit-8 (CCK-8)
was from Dojindo (Kumamoto, Japan). miExpress™ Precursor miRNA Expression
(pEZX-MR03) clone was ordered from GeneCopoeia (Rockville, MD, USA). ...

[HTML] MicroRNA-105 inhibits human glioma cell malignancy by directly targeting SUZ12

J Zhang, W Wu, S Xu, J Zhang, J Zhang, Q Yu, Y Jiao… - Tumor Biology, 2017

Glioma accounts for the majority of primary malignant brain tumors in adults and is highly
aggressive. Although various therapeutic approaches have been applied.

[HTML] Achaete-scute complex homologue-1 promotes development of laryngocarcinoma via facilitating the epithelial–mesenchymal transformation

H Ma, X Du, S Zhang, Q Wang, Y Yin, X Qiu, P Da… - Tumor Biology, 2017

Laryngeal cancer is one of the most common fatal cancers among head and neck carcinomas,
whose mechanism, however, remains unclear. The proneural basic-helix-lo.

[HTML] Silencing of AQP3 induces apoptosis of gastric cancer cells via downregulation of glycerol intake and downstream inhibition of lipogenesis and autophagy

L Chen, Z Li, Q Zhang, S Wei, B Li, X Zhang, L Zhang… - OncoTargets and therapy, 2017

... after transfection and starved (cultured in RPMI 1640 without supplementation with FBS and
penicillin–streptomycin solution) for 48 h. The cell number was estimated with Cell Counting
kit 8 (CCK-8; Dojindo, Kumamoto, Japan) according to the manufacturer's instructions. ...

Lysosome-mediated degradation of a distinct pool of lipid droplets during hepatic stellate cell activation

M Tuohetahuntila, MR Molenaar, B Spee, JF Brouwers… - Journal of Biological …, 2017

... Medium was changed every 3 days. Cell viability and cytotoxicity was assayed with
the Cell Counting Kit-8 according to the instructions provided by the manufacturer
(Dojindo Molecular Technologies, Inc., Rockville, MD, USA). ...

[HTML] Long non-coding RNA MSTO2P promotes the proliferation and colony formation in gastric cancer by indirectly regulating miR-335 expression

H Li, H Zhu, Y Zhou, H Wang, Z Niu, Y Shen, L Lv - Tumor Biology, 2017

Long non-coding RNAs are emerging as new players in gene regulation, but whether long
non-coding RNAs influence the expression of microRNA is unclear. The expre.

Cardiotrophin-1 stimulates the neural differentiation of human umbilical cord blood-derived mesenchymal stem cells and survival of differentiated cells through PI3K/ …

L Peng, X Shu, C Lang, X Yu - Cytotechnology, 2017

... Cell viability assay by Cell Counting Kit-8 (CCK-8). Cell viability assays were
performed using Cell Counting Kit-8 (CCK-8) (Dojindo, Kumamoto, Japan).
CCK-8 was performed according to the manufacturer's instructions. Cells ...

[HTML] LASP2 suppresses colorectal cancer progression through JNK/p38 MAPK pathway meditated epithelial-mesenchymal transition

B Wang, L Zhang, L Zhao, R Zhou, Y Ding, G Li, L Zhao - Cell Communication and …, 2017

... Briefly, 10 µl Cell Counting Kit-8 (Dojindo) was added to each well for 2 h and the
absorbance value (OD) was measured at 490 nm using a microplate reader. All the
experiments were repeated for at least three times. Transwell assay. ...

Sensitivity of mitochondrial DNA depleted ?0 cells to H 2 O 2 depends on the plasma membrane status

K Tomita, Y Kuwahara, Y Takashi, T Tsukahara… - … and Biophysical Research …, 2017

... 2.3. Cell survival assay. The effect of H 2 O 2 on cell proliferation was analyzed by
the water-soluble tetrazolium salt assay using Cell Counting Kit-8 (CCK-8; Dojindo,
Kumamoto, Japan) according to the manufacturer's protocol. ...

The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor

S Kokabu, C Nakatomi, T Matsubara, Y Ono… - Journal of Biological …, 2017

... Cell proliferation assay The proliferation of plated satellite cells were assessed using a Cell
Counting kit-8 (Dojindo, Kumamoto, Japan), according to manufacturer's protocol (50). Chromatin
immunoprecipitation assay - Chromatin immunoprecipitation (ChIP) was ...

[HTML] ANGPTL1 attenuates colorectal cancer metastasis by up-regulating microRNA-138

H Chen, Q Xiao, Y Hu, L Chen, K Jiang, Y Tang, Y Tan… - Journal of Experimental & …, 2017

... Cell proliferation assay. Cell proliferation was analyzed using the Cell Counting
Kit-8 (CCK-8) (Dojindo Laboratories, Tokyo, Japan). Cells were seeded into
96-well plates at the density of 5 × 10 3 cells/200 µl per well. 20 µl ...

JS-K, a GST-activated nitric oxide donor prodrug, enhances chemo-sensitivity in renal carcinoma cells and prevents cardiac myocytes toxicity induced by Doxorubicin

M Qiu, L Ke, S Zhang, X Zeng, Z Fang, J Liu - Cancer Chemotherapy and …, 2017

... Cell viability assay Cell viability was detected with a Cell Counting Kit-8 (CCK-8,
Dojindo, Japan) according to the manufacturer's instructions. Briefly, cells were seeded
in 96-well plates (NEST Biotechnology Co., Ltd, Shanghai ...

Immunomodulatory activity of Ganoderma atrum polysaccharide on purified T lymphocytes through Ca2+/CaN and MAPK pathway based on RNA-seq

QD Xiang, Q Yu, H Wang, MM Zhao, SY Liu, S Nie… - Journal of Agricultural and …, 2017

... Enzyme linked 98 immunosorbent assay (ELISA) kits were from Wuhan Boster Biological
Technology 99 Co. (Wuhan, Hubei, China). Cell counting kit-8 was from Dojindo (Kumamoto,
100 Japan). All antibodies used in flow cytometry were purchased from BD Biosciences 101 ...

Design and development of caffeic acid conjugated with Bombyx mori derived peptide biomaterials for anti-aging skin care applications

H Lee, ATE Vilian, JY Kim, MH Chun, JS Suh, HH Seo… - RSC Advances, 2017

... Gibco, USA). The cells were incubated at 37 °C and 5% CO 2 . The cell viability after
exposure to the different CA–APPPKK was determined using a Cell Counting
Kit-8 (CCK-8) assay (CK04-11, Dojindo, Japan). Fibroblast cells ...

SYNERGISTIC PHARMACEUTICAL COMBINATION FOR THE TREATMENT OF SQUAMOUS CELL CARCINOMA OF HEAD AND NECK

V Agarwal, A Balakrishnan, G Periyasamy - US Patent 20,170,157,090, 2017

... Cisplatin, 5-fluorouracil and docetaxel were obtained from Sigma. CK-8 cytotoxicity kit was
procured from Dojindo Molecular Technologies, Japan. Culture media and fetal bovine serum
(FBS) were obtained from Sigma (St. Louis, Mo.) and Gibco (Paisley, Scotland) respectively. ...

SLC7A11 over-expression in glioblastoma is associated with increased cancer stem-cell like properties

MD Polewski, RF Reveron-Thornton, GA Cherryholmes… - Stem Cells and …, 2017

... analyzed using FlowJo 7.6.1. Cell Viability Assay Cell counting kit-8 (CCK-8; Dojindo Molecular
Technologies) was used to measure cell viability according to the manufacturer's protocol.
Absorbance was measured at 450 nm using a SpectraMax M3. Statistical Analysis ...

[HTML] Optimized Photodynamic Therapy with Multifunctional Cobalt Magnetic Nanoparticles

KH Choi, KC Nam, UH Kim, G Cho, JS Jung, BJ Park - Nanomaterials, 2017

Photodynamic therapy (PDT) has been adopted as a minimally invasive approach for the localized
treatment of superficial tumors, representing an improvement in the care of cancer patients. To
improve the efficacy of PDT, it is important to first select an optimized nanocarrier and ...

MiR-20b inhibits mycobacterium tuberculosis induced inflammation in the lung of mice through targeting NLRP3

J Lou, Y Wang, Z Zhang, W Qiu - Experimental Cell Research, 2017

... 2.8. Cell viability assay. Cells were seeded in 96-well plate, next, the cell viability was
tested using the 10-mL Cell Counting Kit-8 solution (Dojindo Laboratories, Kumamoto,
Japan). 2.9. Enzymes activity detection and elisa assay. ...

[PDF] Identification of rhodamine 123-positive stem cell subpopulations in canine hepatocellular carcinoma cells

H Itoh, S Nishikawa, T Haraguchi, Y Arikawa… - Biomedical Reports, 2017

... Cell proliferation was analyzed using WST-8 and a Cell Counting Kit-8 (CCK-8; Dojindo Molecular
Technologies, Inc.) according to the manufacturer's instructions. Briefly, sorted RhoHi and RhoLo
subpopulations were seeded into 96-well plates at 3x103 cells/well. ...

[HTML] ?-3 free fatty acids and all-trans retinoic acid synergistically induce growth inhibition of three subtypes of breast cancer cell lines

G Lin, S Zhu, Y Wu, C Song, W Wang, Y Zhang… - Scientific Reports, 2017

... evaluate cell viability. Cell Counting Kit 8 (CCK8) was purchased from Dojindo
Molecular Technology (Tokyo, Japan). For CCK8 assay, cells were cultured in
96-well plates at a density of 5000 cells per well in 100 µl medium. ?-3 ...

A novel whey tetrapeptide IPAV reduces interleukin-8 production induced by TNF-a in human intestinal Caco-2 cells

M Oyama, T Van Hung, K Yoda, F He, T Suzuki - Journal of Functional Foods, 2017

... In addition, the viability of Caco-2 cells incubated with or without WPI hydrolysate (2.5 and 5
mg/mL) and IPAV (100 and 200 µM) for 24 h was examined using a commercially available
kit (Cell Counting Kit-8; Dojindo Laboratories, Kumamoto, Japan). 2.7. ...

[HTML] Long noncoding RNA CRNDE stabilized by hnRNPUL2 accelerates cell proliferation and migration in colorectal carcinoma via activating Ras/MAPK signaling …

H Jiang, Y Wang, M Ai, H Wang, Z Duan, H Wang… - Cell Death & Disease, 2017

... The Cell Counting Kit-8 (CCK-8) assay was used to explore the biological effects of CRNDE on
cell proliferation and revealed that the downregulation of ... Cell proliferation was evaluated using
CCK-8 (Dojindo, Rockville, MD, USA) according to the manufacturer's instructions. ...

The cardiac glycoside oleandrin induces apoptosis in human colon cancer cells via the mitochondrial pathway

L Pan, Y Zhang, W Zhao, X Zhou, C Wang, F Deng - Cancer Chemotherapy and …, 2017

... propidium iodide (PI), annexin V-FITC apoptosis detection kit, and 3-(4,5-dimethylthiazol-2-yl)-
2,55-diphenyltetrazo- lium bromide (MTT) (Jiangsu Keygen Biotech Corp., Ltd); cell counting
kit-8 (CCK-8, Dojindo); caspase-3,9 activity kits (Beyotime Institute of Biotechnology ...

The anti-growth and anti-metastasis effects of Schisandrin B on hepatocarcinoma cells in vitro and in vivo

R Sun, R Zhai, C Ma, M Wei - Biochemical and Biophysical Research …, 2017

... 2.2. Cell counting kit-8 assay. Cell Proliferation assay using the cell counting kit-8 (CCK-8; Dojindo,
Japan) was performed according to the manufacturer's instructions. Brie?y, cells were seeded
into 96-well plates at a density of 5000 cells per well with complete growth medium. ...

E2F8 confers cisplatin resistance to ER+ breast cancer cells via transcriptionally activating MASTL

J Tian, Y Lin, J Yu - Biomedicine & Pharmacotherapy, 2017

... after indicating transfection, MCF-7 cells were seeded in a 96-well plate and were further treated
with varying concentrations of cisplatin for 48 h. After that, cell viability was measured using the
Cell Counting Kit-8 (Dojindo, Tokyo, Japan) according to manufacturer's instruction. ...

GSK3 inhibitor AR-A014418 promotes osteogenic differentiation of human adipose-derived stem cells via ERK and mTORC2/Akt signaling pathway

M Zhang, P Zhang, Y Liu, Y Zhou - Biochemical and Biophysical Research …, 2017

... 2.2. Cell proliferation assay. AR-A014418 and U0126 were obtained from Sigma-Aldrich. The
cell proliferative ability was detected with the Cell Counting Kit-8 assay kit (Dojindo, Kumamoto,
Japan). At each time point, the supernatant of each group was discarded. ...

Hydroxycamptothecin prevents intraarticular scar adhesion by activating the PERK signal pathway

X Li, H Chen, Y Sun, J Dai, S Wang, J Wang, L Yan - European Journal of …, 2017

... 2.2. Cell Viability. Cell viability was measured using a Cell Counting Kit-8 (CCK-8; Dojindo,
Tokyo, Japan). The fibroblasts were cultured in triplicate in 96-well plates and treated with
0, 0.05 µg/ml, 0.1 µg/ml, 0.5 µg/ml, 1 µg/ml, 4 µg/ml and 8 µg/ml HCPT. ...

[HTML] Pluronic F127 and D-a-Tocopheryl Polyethylene Glycol Succinate (TPGS) Mixed Micelles for Targeting Drug Delivery across The Blood Brain Barrier

X Meng, J Liu, X Yu, J Li, X Lu, T Shen - Scientific Reports, 2017

... Cells counting kit-8 (CCK-8) were purchase from DOJINDO laboratories (Shanghai, China). ...
excipients (Pluronic F127, TPGS and blank FT mixed micelles) with concentration ranging from
0.1 to 100 µg/ml for 60 h. Cell viability was measured using cell counting kit-8 (CCK-8 ...

[HTML] NLRP3 participates in the regulation of EMT in bleomycin-induced pulmonary fibrosis

R Tian, Y Zhu, J Yao, X Meng, J Wang, H Xie, R Wang - Experimental Cell Research, 2017

... µM. The same volume of CCK-8 solution was added to each well for another 1 h.
The effect of BLM on cell viability was detected using the Cell Counting Kit-8
(CCK-8) (Dojindo, Kumamoto, Kyushu, Japan). The absorbance ...

[PDF] Effects of RNA interference of the eno1 gene on the malignant biological behaviors of gastric cell lines

T Chen, Y Liu, RH Bode Lin, Z Huang - 2017

... Beijing, China). Cell Counting Kit-8 (CCK8) was purchased from DOJINDO Co., Ltd
(Shanghai, China). Primary rabbit ... Beijing, China). Cell Counting Kit-8(CCK8)was
purchased from DOJINDO Co., Ltd (Shanghai, China). Primary rabbit ...

[HTML] Characteristics and potentials of stem cells derived from human degenerated nucleus pulposus: potential for regeneration of the intervertebral disc

XC Li, Y Tang, JH Wu, PS Yang, DL Wang, DK Ruan - BMC Musculoskeletal …, 2017

... Measurement of cell proliferation capacity. To measure the proliferation capacity,
the Cell Counting Kit-8 (CCK-8, Dojindo Laboratories, Japan) and the Passage
4 (P4) cells were used as described previously [27]. Briefly, 10 ...

[HTML] Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264. 7 Cells through Suppression of NF-?B/MAPK Signaling Pathway

Y Li, S He, J Tang, N Ding, X Chu, L Cheng, X Ding… - … -Based Complementary and …, 2017

... 2.3. Microscopic Observation and Cell Counting Kit-8 (CCK-8) Assay for Cell Viability. ... the same
concentration was being performed in triplicate, followed by stimulating with 1 µg/ml LPS in a
37°C, 5% CO 2 incubator for 18 h. A volume of 10 µL CCK-8 (Dojindo, Kumamoto, Japan ...

Nicotinamide induces mitochondrial-mediated apoptosis through oxidative stress in human cervical cancer HeLa cells

Y Feng, Y Wang, C Jiang, Z Fang, Z Zhang, X Lin… - Life Sciences, 2017

... All sterile plastic materials for cell culture were purchased from Corning Incorporated. 2.2. Cell
proliferation assay. Cell proliferation was assessed by Cell Counting Kit-8 (CCK-8, Dojindo,
Molecular Technologies, Japan) as the manufacturer's instruction. ...

[HTML] Bactericidal effects of 310 nm ultraviolet light-emitting diode irradiation on oral bacteria

A Takada, K Matsushita, S Horioka, Y Furuichi, Y Sumi - BMC Oral Health, 2017

... Cytotoxicity to oral epithelial cells. The toxicity of UV-LED irradiation to oral epithelial cells was
measured using the Cell Counting Kit-8 (Dojindo, Tokyo, Japan) of WST-8 assay. Briefly,
Ca9–22 cells were seeded in 96-well tissue culture plates at a density of 2 × 10 4 cells/well. ...

[HTML] Enhanced expression and phosphorylation of Sirt7 activates smad2 and ERK signaling and promotes the cardiac fibrosis differentiation upon angiotensin-II …

H Wang, S Liu, S Liu, W Wei, X Zhou, F Lin, J Wang… - PloS one, 2017

... 2.7. Cell viability assay. Cells were seeded in a 96-well plate as a density of 5000 cells/well
for 24 h. Cell viability was measured using the Cell Counting Kit-8 (Dojindo Molecular
Technologies, Japan), according to the manufacturer's instructions. ...

Direct 3D cell-printing of human skin with functional transwell system

BS Kim, JS Lee, G Gao, DW Cho - 2017

... In addition, the cell proliferation rate was evaluated for 60 h using Cell Counting Kit-8 (CCK-8,
Dojindo, Kumamoto, Japan). The CCK-8 stock solution was diluted (1:10) in cell culture medium
to constitute a working solution, in which the samples were submerged for 150 min. ...

Fabrication of single and bundled filament-like tissues using biodegradable hyaluronic acid-based hollow hydrogel fibers

M Khanmohammadi, S Sakai, M Taya - International Journal of Biological …, 2017

... The growth profiles of recovered cells were estimated by measuring absorbances of
water-soluble formazan dye derived from tetrazolium salt released from the cells using a
colorimetric assay kit (Cell Counting Kit-8; Dojindo, Kumamoto, Japan) [19]. ...

Photoresponsive lipid-polymer hybrid nanoparticles for controlled doxorubicin release

Y Zheng, D Zhang, Z Zhang, X Liu - Nanotechnology, 2017

... DMAP, Sinopharm), doxorubicin hydrochloride (Chembest), sodium carbonate (Na 2 CO 3 ,
Sinopharm), calcium hydride (CaH 2 , Sinopharm), PLGA (Sigma-Aldrich), L-a-
Phosphatidylcholine (Vetec™ reagent grade, Sigma-Aldrich), Cell Counting Kit-8 (CCK-8, Dojindo), ...

Hsp70 protects human trabecular meshwork cells injury induced by UVB through Smad pathway

Y Cao, L Gao, R Tang, W Zhang - Die Pharmazie-An International Journal of …, 2017

... 4.3. Cell proliferation assay TM cells were seeded in 96-well plate with 5000 cells/well.
Cell proliferation was assessed by a Cell Counting Kit-8 (CCK-8, DOJINDO Molecular
Technologies, Gaithersburg, MD) assay. After stimulation ...

miR-152-5p inhibits proliferation and induces apoptosis of liver cancer cells by up-regulating FOXO expression

DL Chang, W Wei, ZP Yu, CK Qin - Die Pharmazie-An International Journal of …, 2017

... HepG2 cells (A) and MHCC97 cells (B). The apoptotic cells rat was detected by fly cytometry
in HepG2 cells (C) and MHCC97 cells (D). CCK-8, Cell Counting Kit-8; ns, no ... Kit-8 (CCK-8, Dojindo,
Kumamoto Prefecture, Kyushu, Japan), according to the manufacturer's instruction. ...

Quinacridone derivative as a new photosensitizer: Photodynamic effects in cells and in vivo

Y Liu, X Hu, L Wang, X Liu, T Bing, W Tan… - Dyes and Pigments, 2017

... pBR322 plasmid DNA was from Takra Biotechnology Co. LTD (Dalian, China). Cell Counting
Kit-8 (CCK-8) and Annexin V-FITC Apoptosis Detection Kit were bought from Dojindo Laboratories.
Reactive Oxygen Species Assay Kit was from Beyotime (Beijing, China). ...

Combined effects of deoxynivalenol and zearalenone on oxidative injury and apoptosis in porcine splenic lymphocytes in vitro

Z Ren, H Deng, Y Deng, Z Liang, J Deng, Z Zuo, Y Hu… - … and Toxicologic Pathology, 2017

... Histopaque 1077 and the Cell Counting Kit-8 (CCK-8) were obtained from Dojindo
Laboratories (Tokyo, Japan). The annexin V-fluorescein isothiocyanate (FITC) Apoptosis
Detection kit was purchased from BD Pharmingen (Lexington, KY, USA). ...

miR-873 suppresses H9C2 cardiomyocyte proliferation by targeting GLI1

JS Zhang, Y Zhao, Y Lv, PY Liu, JX Ruan, YL Sun… - Gene, 2017

... 2.7. Cell proliferation assay. Cell proliferation was measured using a Cell Counting Kit-8
(CCK-8) solution (Dojindo, Kumamoto, Japan) according to the manufacturer's instructions.
The data were measured at 24, 48, 72, 96 and 120 h after transfection. ...

[HTML] The involvement and possible mechanism of pro-inflammatory tumor necrosis factor alpha (TNF-a) in thoracic ossification of the ligamentum flavum

C Zhang, Z Chen, X Meng, M Li, L Zhang, A Huang - PLOS ONE, 2017

... density of 1×10 4 cells/well overnight. Cell proliferation was determined using the
Cell Counting Kit-8 (CCK8) kit (Dojindo, kumamoto, Japan) according to the
manufacturer's instructions. At each time point, the cells were stained ...

Downregulation of lncRNA TUBA4B is Associated with Poor Prognosis for Epithelial Ovarian Cancer

FF Zhu, FY Zheng, HO Wang, JJ Zheng, Q Zhang - Pathology & Oncology Research, 2017

... SKOV3 cell proliferation was assessed with the cell counting kit-8 (CCK-8) assay kit (Dojindo
Molecular Technologies, Inc., Kumamoto, Japan) in accordance with the manufac- turer's
suggestions. Cells were seeded at an initial density of 2 × 104 cells/mL in 96-well plates. ...

Mussel-inspired poly (L-DOPA)-templated mineralization for calcium phosphate-assembled intracellular nanocarriers

HY Nam, KH Min, JR Choi, HJ Lee, SC Lee - Colloids and Surfaces B: Biointerfaces, 2017

... After 24 h and 48 h, the cells were washed with PBS, followed by the cell counting kit (CCK) assay
(Dojindo Laboratories, Kumamoto ... NPs, and DOX-CaP-NPs for 24 h at 37 °C. The cells were
washed twice with PBS, and the medium was replaced with cell counting kit-8 (CCK-8 ...

Labdane diterpene lactones of Vitex pubescens and their antileukemic properties

L Anwar, M Efdi, M Ninomiya, S Ibrahim, DP Putra… - Medicinal Chemistry …, 2017

... CCK-8 assay Cell counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies,
Inc. (Kumamoto, Japan). HL-60 cells (2 × 104 cells/mL, 100 µL) were seeded in 96-well plates.
After 24-h incubation, solutions of test compounds were added into the culture. ...

BERBERINE-URSODEOXYCHOLIC ACID CONJUGATE FOR TREATING THE LIVER

SS Andrali, V Tekumalla - US Patent 20,170,143,742, 2017

... To obtain the data in FIG. 2, and the other IC50 data hereinbelow, Cell Counting
Kit-8 (CCK-8) was used. CCK-8 is commercially available from Dojindo Molecular
Technologies, Inc. CCK-8 allows sensitive colorimetric assays ...

[HTML] Neuroprotective effects of Activin A on endoplasmic reticulum stress-mediated apoptotic and autophagic PC12 cell death

L Xue, H Liu, Y Cui, Y Dong, J Wang, Q Ji, J He, M Yao… - Neural Regeneration …, 2017

... by the Cell Counting Kit-8 (CCK-8) assay. Briefly, PC12 cells were plated in 96-well plates and
differentiated into neurons. After different treatments, the medium was replaced with 100 µL of
normal medium, and 10 µL of CCK-8 solution (Dojindo Laboratories, Kumamoto, Japan ...

Development of an ON/OFF switchable fluorescent probe targeting His tag fused proteins in living cells

K Okitsu, T Misawa, T Shoda, M Kurihara, Y Demizu - Bioorganic & Medicinal …, 2017

... We performed a cytotoxicity assay using cell counting kit-8 according to the manufacturer's
instructions. 23 The results of the cytotoxicity assay revealed that fluorescent probe 1 and
R9-H4A2 did not exhibit marked cytotoxicity against HT1080 cells at 30 µM. ...

In vivo stem cell tracking with imageable nanoparticles that bind bioorthogonal chemical receptors on the stem cell surface

S Lee, HI Yoon, JH Na, S Jeon, S Lim, H Koo, SS Han… - Biomaterials, 2017

It is urgently necessary to develop reliable non-invasive stem cell imaging technology
for tracking the in vivo fate of transplanted stem cells in living subjec.

Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-?B pathway

YJ Wang, HQ Zhang, HL Han, YY Zou, QL Gao… - … and Biophysical Research …, 2017

... Briefly, hBMSCs (5.0 × 103 cells/well) were plated in 96-well plates with various concentrations
of taxifolin (50, 100, 200, 400 µM) for 24 or 48 h. Then, 10 µl of Cell Counting Kit-8 reagent
(Dojindo, Kumamoto, Japan) was added to each plate for 4 h at 37 °C. Absorbance at 450 ...

FBXO50 Enhances the Malignant Behavior of Gastric Cancer Cells

T Miwa, M Kanda, H Tanaka, C Tanaka, D Kobayashi… - Annals of Surgical Oncology, 2017

... Cell Proliferation, Adhesion, Migration, and Invasion Assays. Briefly, cell proliferation
was evaluated using the Cell Counting Kit-8 (Dojindo Molecular Technologies, Inc.,
Kumamoto, Japan). Cells (5 × 10 3 cells per well) were ...

[PDF] KCa3. 1 as an Effective Target for Inhibition of Growth and Progression of Intrahepatic Cholangiocarcinoma

P Song, Y Du, W Song, H Chen, Z Xuan, L Zhao… - Journal of Cancer, 2017

... Cell viability assay Cell counting kit-8 (Dojindo, Japan) was used to detect the
proliferation of Huh28, HUCCT1 cells. These two cell lines were seeded in 96-well
plates and placed in 37°C incubator to allow adherence. Then ...

[HTML] Long noncoding RNA UCA1 induced by SP1 promotes cell proliferation via recruiting EZH2 and activating AKT pathway in gastric cancer

ZQ Wang, Q Cai, L Hu, CY He, JF Li, ZW Quan, BY Liu… - Cell Death & Disease, 2017

... Cell-proliferation and cell cycle analysis. Cell viability was measured using Cell
Counting Kit-8 (Dojindo, Japan). Cell cycle were analyzed by flow cytometry on a
FACScan (Beckman Instruments, Fullerton, CA, USA). The cell ...

Synergistic anti-tumor activity of nimotuzumab in combination with trastuzumab in HER2-positive breast cancer

Y Yang, R Guo, X Tian, Z Zhang, P Zhang, C Li, Z Feng - … and Biophysical Research …, 2017

... CCK-8, Cell Counting Kit 8. 1. Introduction. ... Cells were incubated with increasing concentrations
of trastuzumab, nimotuzumab, or trastuzumab and nimotuzumab in combination. Four days later,
cell proliferation was determined using CCK-8 kit (Dojindo, Japan). ...

[HTML] Preparation and characterization of a novel silicon-modified nanobubble

J Liu, B Zhang, M Li, M Zhou, F Li, X Huang, M Pan… - PloS one, 2017

... Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Tokyo, Japan). The U87 human glioma
cell line was purchased from the American Type Culture Collection. ... Cytotoxicity assay. The
cytotoxicity was assessed by Cell Counting Kit-8 (CCK-8, Dojindo, Kumamoto, Japan). ...

Synergic modulation of inflammatory state of macrophages utilizing anti-oxidant and phosphatidylserine containing polymer-lipid hybrid nanoparticles

MZ Hosain, K Yuzuriha, K Zai, M Takeo, A Kishimura… - MedChemComm, 2017

... 2.6. Assay for cell survival Relative cell viability was measured using a cell counting
kit-8 (WST-8, Dojindo Laboratories, Inc. Japan). Briefly, 1×104 RAW264.7 cells were
seeded in 100 µL of DMEM containing FBS in a 96-well plate. ...

Promising bio-composites of polypyrrole and chitosan: Surface and in vitro biocompatibility performance on 316L SS implants

AM Kumar, B Suresh, S Das, IB Obot, AY Adesina… - Carbohydrate Polymers, 2017

... Cells were allowed to grow on the specimens for 5 and 7 days and then transferred to new
6-well plates, after which an assay was performed as per the Cell Counting Kit-8 (CCK-8)
protocol (Dojindo, Molecular Technologies, Rockville, MD, USA). ...

Notch signaling pathway regulates the growth and the expression of inflammatory cytokines in mouse basophils

S Qu, JJ Lin, J Zhang, LQ Song, XM Yang, C Wu - Cellular Immunology, 2017

... Cells (10 3 /well) were plated in 96-well plates in 200 µL of medium and incubated at 37°C.
Following treatment, reagent from the Cell Counting Kit-8 (CCK-8)10 µL (Dojindo) was added
to wells and incubated for 2 h. The absorbance of the supernatant was measured at 450 nm ...

Enhanced barrier functions and anti-inflammatory effect of cultured coconut extract on human skin

S Kim, JE Jang, J Kim, YI Lee, DW Lee, SY Song… - Food and Chemical …, 2017

... and 1 wt%). Culture medium was replaced every three days. After one, three, and
seven days of culture, the viability of the cells was measured using a cell counting
kit-8 (CCK-8; Dojindo, Kumamoto, Japan). The CCK-8 solution ...

[HTML] Flot2 promotes tumor growth and metastasis through modulating cell cycle and inducing epithelial-mesenchymal transition of hepatocellular carcinoma

CH Wang, XD Zhu, DN Ma, HC Sun, DM Gao, N Zhang… - American Journal of Cancer …, 2017

... Cell proliferation, migration, and invasion assays. The proliferation of HCC cells was evaluated
using a Cell Counting Kit-8 (CCK-8, Dojindo, Kumamoto, Japan) assay. Cells were plated in
a 96-well plate at 2×10 3 cells per well and allowed to grow for different times. ...

[HTML] Histidine deficiency attenuates cell viability in rat intestinal epithelial cells by apoptosis via mitochondrial dysfunction

T Matsui, H Ichikawa, T Fujita, T Takagi, M Osada-Oka… - Journal of Nutrition & …, 2017

... Cell viability was evaluated with Cell counting kit-8 (Dojindo Laboratories, Kumamoto, Japan)
following the manufacturer's instructions [17]. Absorbance was read at 450 nm using the
SpectraMax 190 Microplate Reader (Molecular devices, Tokyo, Japan). ...

Circular RNA hsa_circ_0010729 regulates vascular endothelial cell proliferation and apoptosis by targeting the miR-186/HIF-1a axis

RY Dang, FL Liu, Y Li - Biochemical and Biophysical Research …, 2017

... 2.9. Cell proliferation assay. Cell proliferation assay was performed to detect cell viability
using Cell Counting Kit-8 (CCK-8, DOJINDO). Briefly, HUVECs were cultured in 96-well
plates at a density of 1 × 10 4 cells per well. Subsequently ...

[HTML] Regulation of Matrix Metalloproteinase-2 Secretion from Scleral Fibroblasts and Retinal Pigment Epithelial Cells by miR-29a

Y Zhang, DN Hu, Y Zhu, H Sun, P Gu, D Zhu, J Zhou - BioMed Research International, 2017

... The effects of miR-29a on the growth of scleral fibroblasts and RPE cells were assessed using
the cell counting kit-8. The changes in MMP-2 ... effects of miR-29a on scleral fibroblast and RPE
cell growth were assessed using a cell counting kit (CCK8; Dojindo, Kumamoto, Japan ...

Identification of MicroRNAs Involved in Resistance to Sunitinib in Renal Cell Carcinoma Cells

N YAMAGUCHI, M OSAKI, K ONUMA, T YUMIOKA… - Anticancer Research, 2017

... Cell proliferation assay. Cell proliferation of ACHN and SR-ACHN cells, as well
as RCC23 and SR-RCC23 cells, was examined using a Cell Counting Kit 8 (Dojindo
Molecular Technologies, Kumamoto, Japan). Cells (5×10 ...

Comparison of chemical structures and cytoprotection abilities between direct and indirect antioxidants

S Joko, M Watanabe, H Fuda, S Takeda, T Furukawa… - Journal of Functional Foods, 2017

... Dual-Glo Luciferase Assay System, FuGENE HD Transfection Reagent, ReliaPrep RNA Cell
Miniprep System, GoScript Reverse Transcription System (Promega, Madison, WI, USA); Cell
Counting Kit-8 (CCK-8), GSSG/GSH Quantification Kit (Dojindo Molecular Technologies ...

[HTML] MicroRNA-365 inhibits ovarian cancer progression by targeting Wnt5a

Y Wang, C Xu, Y Wang, X Zhang - American Journal of Cancer Research, 2017

... The relative expression of miR-365 and Wnt5a was analyzed by the 2 -??Ct method.
Cell proliferation and colony formation assays. Cell proliferation was assessed using
the Cell Counting Kit-8 (CCK-8, Dojindo, Kumamoto, Japan). ...

[PDF] Combination of icotinib and wogonin induces apoptosis and autophagy to overcome acquired resistance in lung cancer harbouring EGFR T790M mutation

H Zhang, C Ge, C Lin, L Yi, J Ran, X Shi, C Tang, Y Wu… - Int J Clin Exp Med, 2017

... Evaluation of the inhibitory activity Rate of cell inhibition was eva- luated using the
cell counting kit-8 (CCK-8) (Dojindo, Kuma- moto, Japan) according to the
manufacturer's protocol. Bri- efly, at the end of the treat- ment, 10 µL ...

[PDF] MicroRNA-520f represses non-small cell lung cancer progression by inhibiting TM4SF1

Y Liu, S Zhao, M Song, H Zhang - Int J Clin Exp Med, 2017

... Proliferation assay We used the Cell Counting Kit-8 (CCK-8, Dojindo, Kumamoto, Japan)
to quantify prolif- eration. After treatment for 24 h, H1299 and H522 cells were suspended
and seeded into a 12-well plate (105 cells/well) for 5 days. ...

Effects of interleukin-17 on human retinal vascular endothelial cell capillary tube formation in vitro

G Liu, H Wu, L Chen, J Xu, M Wang, D Li, P Lu - Molecular Medicine Reports

... no. NBP2-27338; clone 4H1524) and human recombinant IL-17 protein (cat. no.
NBP2-35040-25 µg) were purchased from Novus Biologicals (Littleton, CO, USA). The cell
counting kit-8 was purchased from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). ...

Metformin enhances the cytotoxicity of 5-aminolevulinic acid-mediated photodynamic therapy in vitro

T Osaki, I Yokoe, K Takahashi, K Inoue, M Ishizuka… - Oncology Letters

... Subsequently, the cells were incubated at 37°C for 24 h in the dark. The viability of KLN205
cells was examined using the Cell Counting Kit-8 (Dojindo Molecular Technologies, Inc.,
Kumamoto, Japan) according to the manufacturer's instruc- tions. ...

Involvement of the TGFß1/Smad2/MMP3 signaling pathway in SB431542-induced inhibition of cell invasion in multiple myeloma RPMI 8226 cells

H Xi, QG Shuai, L SHAO - Oncology Letters

... Cell counting kit-8 (CCK-8) cell proliferation assay. ... incubated at 37°C in the presence and absence
of SB431542 (1.0, 10, 100 and 1,000 nmol/ml; Med Chem Express Co., Monmouth Junction,
NJ, USA) for 12, 24 and 48 h. CCK-8 solution (10%; Dojindo Molecular Tech ...

VPA and MEL induce apoptosis by inhibiting the Nrf2-ARE signaling pathway in TMZ-resistant U251 cells

H Pan, H Wang, Y Jia, Q Wang, L Li, Q Wu, L Chen - Molecular Medicine Reports

... Following this stepwise increase in TMZ concentration for ~8 months, the final U251-TMZ
cell line was able to survive in 100 µM TMZ. Subsequently, Cell Counting kit-8 (CCK-8;
Dojindo Molecular Technologies, Inc., Kumamoto, Japan) was ...