Cell Counting Kit-8
March 2017 Publications~

[PDF] Novel Akt activator SC-79 is a potential treatment for alcohol-induced osteonecrosis of the femoral head

YX Chen, SC Tao, ZL Xu, WJ Yin, YL Zhang, JH Yin…

... Cell toxicity and proliferation The effect of ethanol and Ly294002 on the proliferation
of BMSCs was assessed using a cell viability assay (Cell Counting Kit-8 (CCK-8),
Dojindo Molecular Technologies, Inc., Japan). Briefly, BMSCs ...

The activation of Akt/mTOR pathway by bleomycin in Epithelial-to-mesenchymal transition of human submandibular gland cells: A treatment mechanism of bleomycin …

Y Cai, R Sun, R Wang, JG Ren, W Zhang, YF Zhao… - Biomedicine & …, 2017

... St. Louis, MO, USA) were added in the media, and then the viability of the cultured
cells was measured using the Cell Counting Kit-8 assay (Dojindo, Kyushu, Japan)
as previously described [13]. 2.3. Evaluation of apoptosis. ...

High STMN1 level is associated with chemo-resistance and poor prognosis in gastric cancer patients

T Bai, T Yokobori, B Altan, M Ide, E Mochiki, M Yanai… - British Journal of Cancer, 2017

... The cells were seeded in 96-well plates (approximately 3000 cells per well in 100 µl of medium
containing 10% FBS). After 0 h, 24 h, 48 h, and 72 h, cell proliferation was measured with the
Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Tokyo, Japan). ...

Monascus ruber-Fermented Buckwheat (Red Yeast Buckwheat) Suppresses Adipogenesis in 3T3-L1 Cells

H Hong, J Park, WL Lumbera, SG Hwang - Journal of Medicinal Food, 2017

... Determination of cell viability We determined the effect of different concentrations
of RYB on 3T3-L1 preadipocyte viability using a Cell Counting Kit-8 (CCK-8) assay
(Dojindo Molecular Technologies, Tokyo, Japan). In brief, the ...

[HTML] miR-18a counteracts AKT and ERK activation to inhibit the proliferation of pancreatic progenitor cells

X Li, Z Zhang, Y Li, Y Zhao, W Zhai, L Yang, D Kong… - Scientific Reports, 2017

... CCK8 assay and cell counting. Cell number was assayed using cell counting
kit-8 (CCK8) (Dojindo Laboratories, Japan). After the pancreatic progenitor cells
were transfected for 72 h, 10 µL CCK8 reaction solution was added ...

Odontoclastogenesis of mouse papilla-derived MDPC-23 cells induced by LPS

C Li, WT Qi, HW Jiang - International Endodontic Journal, 2017

... kit-8 (CCK8) MDPC-23 cells were seeded at a density of 1.5×104 cells/mL in a 96-well plate with
a-MEM containing 10% FBS (with or without LPS treatment), and blank group without cells was
also prepared. After 2, 4 and 6 days, 10 µL of CCK-8 solution (DojinDo, Kumamoto ...

[PDF] HOXA10 promotes nasopharyngeal carcinoma cell proliferation and invasion via inducing the expression of ZIC2

ZH SHEN, KM ZHAO, T Du - European Review for Medical and Pharmacological …, 2017

... a 96-well plate (3×103 cells per well) and were further cultured for 3 days. Then,
cell viability was measured by WST-8 assay using Cell Counting Kit-8 (CCK-8,
Dojindo, Tokyo, Ja- pan) following the manufacturer's protocol. ...

Linc00152 promotes cancer progression in hepatitis B virus-associated hepatocellular carcinoma

X Deng, X fang Zhao, X qiu Liang, R Chen, Y feng Pan… - Biomedicine & …, 2017

... 2.5. Cell proliferation assays and cell invasion assays. The cells were plated in
96-well plates, and measured using Cell Counting Kit-8 (Dojindo, Japan). After cell
transfection, the cell proliferation ability was monitored at 0 h ...

Expression, purification and characterization of active untagged recombinant human leukemia inhibitory factor from E coli

X Xi, X Li, F Wu, X Guan, L Jin, Y Guo, W Song, B Du - Protein Expression and …, 2017

... for purification purpose. M1 cells were purchased from ATCC. Cell Counting Kit-8
(CK04) was obtained from DOJINDO LABORATORIES. DMEM and Fetal bovine
serum were purchased from Life technology. IPTG (Isopropyl ß ...

[PDF] Glioma cells promote angiogenesis through the release of exosomes containing long non-coding RNA POU3F3

HL LANG, GW HU, Y Chen, Y Liu, W Tu, YM LU, L Wu… - European Review for …, 2017

... Endothelial Cell Proliferation Assay A Cell Counting Kit-8 assay (CCK-8; Dojindo,
Kumamoto, Japan) was used to assess cell proli- feration. Briefly, HBMECs were
seeded at 5 × 104 cells/mL (100 µL/well) in a 96-well plate. ...

[HTML] FNC efficiently inhibits mantle cell lymphoma growth

Y Zhang, R Zhang, X Ding, B Peng, N Wang, F Ma… - PloS one, 2017

... For in vivo experiments, FNC and Ara-C were dissolved in 0.9% sodium chloride.
Cell proliferation analysis. Cell viability was determined using the Cell Counting
kit-8 (Dojindo Company, Japan)[17]. Briefly, 2×10 4 JeKo-1 cells ...

Thermosensitive hydrogel loaded with chitosan-carbon nanotubes for near infrared light triggered drug delivery

X Dong, C Wei, J Liang, T Liu, D Kong, F Lv - Colloids and Surfaces B: Biointerfaces, 2017

... The cell toxicity of CNTs-CS or CNTs on L929 and BEL-7402 cells was evaluated with Cell
Counting Kit-8 (Dojindo Molecular Technologies, Inc., Kumamoto, Japan) after incubation for
24 hours with different concentrations of CNTs-CS or CNTs (0.625 µg/mL,1.25 µg/mL,2.5 µg ...

MicroRNA-802 plays a tumour suppressive role in tongue squamous cell carcinoma through directly targeting MAP2K4

X Wu, Z Gong, L Sun, L Ma, Q Wang - Cell Proliferation, 2017

... 2.3 Cell viability and cell invasion assays. Cell Counting Kit-8 (CCK8; Dojindo, Tokyo,
Japan) was used for cell viability. Cell was cultured on the 96-well plate and the number
of cells was measured by measurement of an absorbance ...

TiO 2 nanorod arrays modified Ti substrates promote the adhesion, proliferation and osteogenic differentiation of human periodontal ligament stem cells

Z Li, J Qiu, LQ Du, L Jia, H Liu, S Ge - Materials Science and Engineering: C, 2017

... A cell counting kit-8 (CCK8; Dojindo, Tokyo, Japan) was used to evaluate the
proliferation of PDLSCs. Briefly, after incubated for 3, 5 and 7 days, the substrates were
transferred to a new 24-well plate. Then 500 µl DMEM containing ...

[PDF] MicroRNA-122 inhibits proliferation and invasion in gastric cancer by targeting CREB1.

M Rao, Y Zhu, Y Zhou, X Cong, L Feng - American Journal of Cancer Research, 2017

... Cell proliferation, migration, and invasion as- says Cell proliferation was determined using
Cell Counting Kit-8 assay kits (CCK-8, Dojindo, Kumamoto, Japan). Briefly, transfected cells
were seeded into 24-well plates at a density of 5 × 103 cells/well. ...

[PDF] The involvement of senescence induced by the telomere shortness in the decline of osteogenic differentiation in BMSCs

C Li, GJ WEI, L Xu, JS RONG, SQ TAO, YS WANG - European Review for Medical and …, 2017

... kit (Beyotime Biotech Ltd., Co, Shanghai, China); reagents used in cell culture, including DMEM
(Dulbecco's modified Eagle medium), fetal bovine serum (FBS), and 0.25% trypsin (Gibco, Grand
Island, NY, USA); CCK-8 (Cell Counting Kit-8, Dojindo, Japan); alkaline ...

Block copolymer-boron cluster conjugate for effective boron neutron capture therapy of solid tumors

P Mi, H Yanagie, N Dewi, HC Yen, X Liu, M Suzuki… - Journal of Controlled …, 2017

... (Tokyo, Japan). Cell Counting Kit-8 (CCK-8) was bought from Dojindo Molecular Technologies,
Inc. (Kumamoto, Japan). Hoechst 33342, Alexa647- and Alexa488-succinimidyl esters were
bought from Life Technologies Japan Co., Ltd. (Tokyo, Japan). ...

MicroRNA-488-3p sensitizes malignant melanoma cells to cisplatin by targeting PRKDC

N Li, Y Ma, L Ma, Y Guan, L Ma, D Yang - Cell Biology International, 2017

... After transfected with miR-488-3p, 2 × 103 cells were seeded in 96-well culture plates, cells were
tested for proliferation every 24 h using the Cell Counting Kit-8 (CCK-8, Dojindo, and Kumamoto,
Japan) and the A450 values were measured with a microplate reader. ...

5'-triphosphate siRNA targeting MDR1 reverses multi-drug resistance and activates RIG-I-induced immune-stimulatory and apoptotic effects against human myeloid …

D Li, RP Gale, Y Liu, B Lei, Y Wang, D Diao, M Zhang - Leukemia Research, 2017

... All data were analyzed using FlowJo software (Version7.6.5, Tree Star, Ashland, OR, USA).
2.7. Cell viability assay. Cell viability was determined by Cell Counting Kit-8 (CCK8) assay
(Dojindo Molecular Technologies Inc., Shanghai, China). ...

[HTML] HCMV-encoded miR-UL112-3p promotes glioblastoma progression via tumour suppressor candidate 3

Q Liang, K Wang, B Wang, Q Cai - Scientific Reports, 2017

... All of the assays were performed 48 hours after transfection. Cell viability. Cell viability
was monitored using cell counting kit-8 (CCK-8; Dojindo Laboratories, Kumamoto, Japan).
Briefly, 5 × 10 3 cells/well were plated into 96-well microplates. ...

[HTML] OX40 signaling is involved in the autoactivation of CD4+ CD28- T cells and contributes to the pathogenesis of autoimmune arthritis

J Jiang, C Liu, M Liu, Y Shen, X Hu, Q Wang, J Wu… - Arthritis Research & …, 2017

... Rat immunoglobulin G (IgG) (clone RTK4530; BioLegend) was added as a control.
After 72 h of incubation, cell proliferation was determined using a Cell Counting
Kit-8 (CCK-8; Dojindo Laboratories, Mashikimachi, Japan). ...

Effect of Momordica charantia protein on proliferation, apoptosis and the AKT signal transduction pathway in the human endometrial carcinoma Ishikawa H cell line in …

HZ Gu, RR Lin, H WANG, X ZHU, Y Hu, F ZHENG - system

... viability in various concentrations of MCP30 (0, 8.33, 16.67, 33.33, 166.67, 333.33 and 666.67
pM; these concentrations were selected due to preliminary experiments) for 24, 48 and 72 h was
assessed using the Cell Counting Kit-8 (CCK-8 kit; Dojindo Laboratories, Kumamoto ...

Xiaoaiping combined with cisplatin can inhibit proliferation and invasion and induce cell cycle arrest and apoptosis in human ovarian cancer cell lines

AW Zheng, YQ Chen, J Fang, YL Zhang, DD Jia - Biomedicine & Pharmacotherapy, 2017

... Annexin-V-PI Apoptosis Detection Kit and Cell counting kit-8 (CCK-8) were provided by
Dojindo (Kumamoto, Japan). The human ovarian cancer HO-8910 and HO-8910PM cell
lines were obtained from Zhejiang Cancer Research Institute, China. ...

Up regulation of IL-6 is involved in di (2-ethylhexyl) phthalate (DEHP) induced migration and invasion of non small cell lung cancer (NSCLC) cells

Y Wang, M Zhao, J Liu, J Ni, Y Jiao, C Bai - Biomedicine & Pharmacotherapy, 2017

... 2.3. Cell proliferation assay. The cell counting kit-8 (CCK8, Dojindo, Kumamoto, Japan)
was used to test the effects of DEHP on the proliferation of NSCLC cells according
to the manufacturer's instructions. Briefly, A549 or H1299 ...

Claudin-1 regulates pulmonary artery smooth muscle cell proliferation through the activation of ERK1/2

X Cheng, Y Wang, H Chen, Y Xu, W Xiong, T Wang - Biomedicine & …, 2017

... GAPDH was used as endogenous control. 2.9. Cell counting kit-8 assay (CCK-8). CCK-8 was
obtained from Dojindo Laboratory (Kumamoto, Japan). In brief, human PASMCs were seeded
in 96-well culture plates (3 × 10 3 cells/well) and allowed to adhere overnight. ...

LncRNA PVT1 epigenetically silences miR-195 and modulates EMT and chemoresistance in cervical cancer cells

CJ Shen, YM Cheng, CL Wang - Journal of Drug Targeting, 2017

... h after transfection. Then, the cells were treated with 10 nM PTX for 72 h. After that, cell
viability was detected using Cell Counting Kit-8 (CCK-8, Dojindo, Kumamoto, Japan)
according to the manufacturer's instructions. The cell viability curves were ...

Dickkopf 3 attenuates xanthine dehydrogenase expression to prevent oxidative stress-induced apoptosis

S Qui, J Kano, M Noguchi - Genes to Cells, 2017

... Cells were cultured for 48 h after siRNA transfection, treated with Cell Proliferation Reagent
WST-1 (Roche Applied Science, Mannheim, Germany) or Cell Counting Kit-8 (DOJINDO,
Kumamoto, Japan) in 10% v/v medium and then incubated for 2 h at 37 °C. The ...

IL-22 suppresses the infection of porcine enteric coronaviruses and rotavirus by activating STAT3 signal pathway

M Xue, J Zhao, L Ying, F Fu, L Li, Y Ma, H Shi, J Zhang… - Antiviral Research, 2017

... The range of detection for the IL-22 ELISA is 62.5–1000 pg/ml. 2.8. Cytotoxicity assay. The
cytotoxicity of purified mpIL-22 or S3I-201 in IPEC-J2 cells was measured using a Cell
Counting Kit-8 (CCK-8) (Dojindo, China) according to the kit instructions. ...

[HTML] Nanostructured lipid carrier (NLC)-based novel hydrogels as potential carriers for nepafenac applied after cataract surgery for the treatment of inflammation: design, …

S Yu, G Tan, D Liu, X Yang, W Pan - RSC Advances, 2017

... Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F-12), fetal bovine serum
(FBS) and trypsin were all obtained from Gibco Technology (Shanghai, China). Cell Counting
Kit-8 (CCK-8) was acquired from Dojindo Molecular Technology Inc. (Shanghai, China). ...

Characterization of dormant and active human cancer cells by quantitative phase imaging

P Guo, J Huang, MA Moses - Cytometry Part A, 2017

... Cell Proliferation Assays. Cell proliferation assays were performed using the Cell
Counting Kit-8 (CCK-8) (Dojindo Molecular Technologies, MD, USA) according to
manufacturer's instructions as previously reported by us [24-27]. ...

[PDF] Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats

Y Zhao, Z Jia, S Huang, Y Wu, L Liu, L Lin, D Wang… - Stem Cells International, 2017

... 4.5. Proliferation Assay. The proliferation capacity of young and old NPMSCs was
determined using the Cell Counting Kit-8 (CCK-8, Dojindo Laboratories, Kumamoto,
Japan) according to the manufacturer's protocol. Briefly, P3 ...

[HTML] Identification of Mesencephalic Astrocyte-Derived Neurotrophic Factor as a Novel Neuroprotective Factor for Retinal Ganglion Cells

FJ Gao, JH Wu, TT Li, SS Du, Q Wu - Frontiers in Molecular Neuroscience, 2017

... The stained sections and cells were visualized and imaged using confocal microscopy (Leica
SP8, Hamburg, Germany). Cell Counting Kit-8 (CCK8) Assay for RGC Viability. CCK8 solution
(Dojindo Laboratories, Kumamoto, Japan) was added to 96-well plates (10 µl/well). ...

[HTML] MicroRNA-532 and microRNA-3064 inhibit cell proliferation and invasion by acting as direct regulators of human telomerase reverse transcriptase in ovarian cancer

L Bai, H Wang, AH Wang, LY Zhang, J Bai - PLOS ONE, 2017

... After 72 hours, 10 µl of Cell counting kit-8 solution (Dojindo, Japan) was added to each well and
incubated at 37°C for 2 hours; followed by the measurement of absorbance using an automated
micro-plate reader (Corona Electric) at 450 nm. In vitro transwell invasion assay. ...

RIPK1/RIPK3/MLKL-mediated necroptosis contributes to compression-induced rat nucleus pulposus cells death

S Chen, X Lv, B Hu, Z Shao, B Wang, K Ma, H Lin… - Apoptosis, 2017

... Receptor-interacing protein kinase 1 RIPK3 Receptor-interacing protein kinase 3 MLKL Mixed
lineage kinase domain-like CCK-8 Cell counting kit-8 PI Propidium ... 1.0 MPa pressure for 0, 12,
24, 36 or 48 h, and measured using a Cell Counting Kit (CCK-8, Dojindo, Japan) accord ...

Gene delivery of TIPE2 inhibits breast cancer development and metastasis via CD8+ T and NK cell-mediated antitumor responses

Z Zhang, L Liu, S Cao, Y Zhu, Q Mei - Molecular Immunology, 2017

... 2.5. Cell proliferation assays. Cell proliferation was determined by cell counting
kit-8 (CCK-8, Dojindo, Japan) as described by the manufacturer's instructions.
4T1/vector and 4T1/TIPE2 cells (3000 per well) were plated in 96 ...

Anti-osteoclastogenesis of Mineral Trioxide Aggregate through Inhibition of the Autophagic Pathway

X Cheng, L Zhu, J Zhang, J Yu, S Liu, F Lv, Y Lin, G Liu… - Journal of Endodontics, 2017

... hours). Cell counting kit-8 (CCK-8) (Dojindo, Kumamoto, Japan) was performed
following the manufacturer's protocol. The reagent (from CCK-8) mixed with a-MEM
(1:10) was added before incubation at 37°C for another 2 hours. ...

[HTML] Microglia-derived IL-1ß contributes to axon development disorders and synaptic deficit through p38-MAPK signal pathway in septic neonatal rats

Q Han, Q Lin, P Huang, M Chen, X Hu, H Fu, S He… - Journal of …, 2017

... The purity of primary neuron cultures in this study was above 95%. Treatment of primary
cultured neurons. To determine the IL-1ß concentration, the CCK-8 assay (cell counting kit
8) was performed following the manufacturer's instructions (DOJINDO). ...

[PDF] Mutual Regulation of MiR-199a-5p and HIF-1a Modulates the Warburg Effect in Hepatocellular Carcinoma

B Li, L He, D Zuo, W He, Y Wang, Y Zhang, W Liu… - Journal of Cancer, 2017

... All results were normalized with cell number. Cell proliferation assay and colony formation
assay The cell proliferation was determined by way of WST-8 staining with Cell Counting
Kit-8 (CCK-8, Dojindo, Japan) following the manufacturer's protocol. ...

Protective effect of polyacetylene from Dendropanax morbifera Leveille leaves on pulmonary inflammation induced by cigarette smoke and lipopolysaccharide

JW Lee, HW Ryu, SU Lee, TH Son, HA Park, MO Kim… - Journal of Functional Foods, 2017

... Cell viability was then measured in triplicate using the Cell Counting Kit-8 (CCK-8, Dojindo
Molecular Technologies, Rockville, ML) according to the manufacturer's protocol. Absorbance
was measured using a VERSA max microplate reader (Molecular Devices, Sunnyvale, CA). ...

Increased semaphorin 3c expression promotes tumor growth and metastasis in pancreatic ductal adenocarcinoma by activating the ERK1/2 signaling pathway

X Xu, Z Zhao, S Guo, J Li, S Liu, Y You, B Ni, H Wang… - Cancer Letters, 2017

... A CCK-8 (Cell Counting Kit-8) (Dojindo, Kumanoto, Japan) cell proliferation assay was conducted
according to the manufacturer's protocol. The OD450 was measured by spectrophotometry (Bio
Tek, Vermont, USA) after a 2-h incubation with 20 µl of CCK-8 reagent. 2.9. ...

[HTML] Enhanced osteogenesis and angiogenesis by mesoporous hydroxyapatite microspheres-derived simvastatin sustained release system for superior bone regeneration

WL Yu, TW Sun, C Qi, HK Zhao, ZY Ding, ZW Zhang… - Scientific Reports, 2017

Biomaterials with both excellent osteogenic and angiogenic activities
are desirable to repair massive bone defects.

An osteogenesis/angiogenesis-stimulation artificial ligament for anterior cruciate ligament reconstruction

H Li, J Li, J Jiang, F Lv, J Chang, S Chen, C Wu - Acta Biomaterialia, 2017

... The cell viability at different time point (1, 3 and 7 days) in each group (PET, BG/PET,
Cu-BG/PET, Cu-BG/PET+siRNA and Cu-BG/PET+NC group) was assessed with Cell Counting

[HTML] Induction of immunogenic cell death of tumors by newly synthesized heterocyclic quinone derivative

K Son, K ryung Choi, CK Ryu, SJ Lee, HJ Kim, H Lee - PloS one, 2017

... At the end of each incubation period, the cultured wells were then treated with 20 µl/well of Cell
Counting Kit-8 solution (Dojindo Laboratories) for last 3 hr and the optical density of wells was
measured at 450 nm using a microplate reader (Bio-Tek). Generation of DCs in vitro. ...

Autophagy Inhibits C2-ceramide-mediated Cell Death by Decreasing the Reactive Oxygen Species Levels in SH-SY5Y Cells

C Fan, Y Liu, M Zhao, R Zhan, W Cui, H Jin, Y Teng… - Neuroscience Letters, 2017

... Complete medium plus sterile DMSO was used as the control group. 2.2. Cell viability and
clonogenic assays. Cell viability was evaluated using the cell counting kit-8 (CCK-8) assay
[22], according to the manufacturer's protocol (Dojindo, Japan). ...

Nanocapsules of therapeutic proteins with enhanced stability and long blood circulation for hyperuricemia management

X Zhang, D Xu, X Jin, G Liu, S Liang, H Wang, W Chen… - Journal of Controlled …, 2017

... purchased from Sigma and used as received. Cell Counting Kit-8 was obtained from
Dojindo Laboratories. Clear polystyrene 12-well and 96-well tissue culture plates were
obtained from Corning Costar. Male Balb/c and Kun Ming ...

MiR-216b increases cisplatin sensitivity in ovarian cancer cells by targeting PARP1

Y Liu, Z Niu, X Lin, Y Tian - Cancer Gene Therapy, 2017

... well plates; 24 h later, cisplatin (Sigma Chemicals, St Louis, MO, USA) was added at various
concentrations of 0, 2.5, 5, 20, 40 and 80 µm. After 48 h of incubation, cell viability was assessed
using the Cell Counting Kit 8 (CCK-8) (Dojindo Laboratories, Kumamoto, Japan) assay. ...

MR image-guided delivery of cisplatin-loaded brain-penetrating nanoparticles to invasive glioma with focused ultrasound

KF Timbie, U Afzal, A Date, C Zhang, J Song, GW Miller… - Journal of Controlled …, 2017

... Cells were incubated for 3 days at 37 °C and 5% CO 2 . To quantify the number of live cells, media
was replaced with 100 µL of fresh medium and 10 µL of Dojindo Cell Counting Kit-8 (Dojindo
Molecular Technologies, Inc., Rockville, MD) solution was subsequently added. ...

[HTML] Chitosan-Functionalized Graphene Oxide as a Potential Immunoadjuvant

T Yan, H Zhang, D Huang, S Feng, M Fujita, XD Gao - Nanomaterials, 2017

The application of graphene oxide (GO) as a potential vaccine adjuvant has recently attracted
considerable attention. However, appropriate surface functionalization of GO is crucial to improve
its biocompatibility and enhance its adjuvant activity. In this study, we developed a simple ...

Long non-coding RNA SNHG20 predicts a poor prognosis for HCC and promotes cell invasion by regulating the epithelial-to-mesenchymal transition

J Liu, C Lu, M Xiao, F Jiang, L Qu, R Ni - Biomedicine & Pharmacotherapy, 2017

... 2.5. Cell counting kit-8 tests (CCK8) and transwell invasion assays. Cell proliferation
ability was detected using the CCK-8 (Cell Counting Kit-8) assay (Dojindo, Kumamoto,
Japan) according to the manufacturer's protocol. Cells ...

[PDF] DNA-PK Inhibition by NU7441 Enhances Chemosensitivity to Topoisomerase Inhibitor in Non-Small Cell Lung Carcinoma Cells by Blocking DNA Damage Repair

M Yanai, H Makino, B Ping, K Takeda, N Tanaka… - Yonago Acta Medica, 2017

... Cell Counting Kit-8 (CCK-8) assay A CCK-8 assay was performed to evaluate cell prolifer- ation
inhibition. ... After 72 h of drug treatment, 10% CCK-8 solution (Dojindo, Kumamoto, Japan) was
added to each well and incubation was continued for an addi- tional 3 h. The ...

A yellowish-green-light-controllable nitric oxide donor based on N-nitrosoaminophenol applicable for photocontrolled vasodilation

H Okuno, N Ieda, Y Hotta, M Kawaguchi, K Kimura… - Organic & Biomolecular …, 2017

... Kumamoto, Japan). The cell viability assay was conducted using the Cell Counting
Kit-8 (DOJINDO LABOLATORIES, Kumamoto, Japan). ESR spectra were recorded
on a JES-RE2X spectrometer (JEOL Co. Ltd, Tokyo, Japan). MGD ...

[HTML] Individual and Combined Cytotoxic Effects of Co-Occurring Deoxynivalenol Family Mycotoxins on Human Gastric Epithelial Cells

Y Yang, S Yu, Y Tan, N Liu, A Wu - Toxins, 2017

Mycotoxin contamination is a significant health concern for human beings, but health risk
assessments are usually based on one single mycotoxin, which might neglect the additive
or competitive interactions between co-occurring mycotoxins [...].

[HTML] Salinomycin repressed the epithelial–mesenchymal transition of epithelial ovarian cancer cells via downregulating Wnt/ß-catenin pathway

R Li, T Dong, C Hu, J Lu, J Dai, P Liu - OncoTargets and therapy, 2017

... The cell counting kit 8 (CCK-8) and Boyden chamber assays showed that salinomycin could
effectively reduce the abilities of proliferation, migration and invasion in EOC cells. ... After incubating
for 24, 48, 72 h, cell viability was measured by CCK-8 (Dojindo, Kumamoto, Japan). ...

[HTML] Blockage of Glyoxalase I Inhibits Colorectal Tumorigenesis and Tumor Growth via Upregulation of STAT1, p53, and Bax and Downregulation of c-Myc and Bcl-2

Y Chen, L Fang, J Zhang, G Li, M Ma, C Li, J Lyu… - International Journal of …, 2017

... RNA interference was used to deplete GLOI activity in four CRC cell lines. The cells'
proliferation, apoptosis, migration, and invasion were assessed by using the Cell Counting
Kit-8, plate colony formation assay, flow cytometry, and transwell assays. ...

[PDF] Gambogenic acid synergistically potentiates bortezomib-induced apoptosis in multiple myeloma

R Chen, H Zhang, P Liu, X Wu, B Chen - Journal of Cancer, 2017

... and BTZ (Selleck, Houston, TX, USA) were dissolved in dimethyl sulfoxide (DMSO) (Sigma
Chemical Co., USA) to 100mM and stored at -20°C. Cell counting kit-8 (CCK-8) was purchased
from Dojindo, Kumamoto, Japan and was dissolved in phosphate buffered saline (PBS). ...

The role of the progressive ankylosis protein (ANK) in adipogenic/osteogenic fate decision of precursor cells

T Minashima, M Quirno, YJ Lee, T Kirsch - Bone, 2017

... The plate was incubated for 12 h, 24 h or 48 h in a humidified incubator at 37 °C followed by
the addition of 10 µl Cell counting Kit-8 (CCK-8; Dojindo Molecular Technologies, Inc., Rockville,
MD) and incubation for 4 h at 37 °C. After the incubation, the absorbance of the ...

Long non-coding RNA UCA1 regulates the expression of Snail2 by miR-203 to promote hepatocellular carcinoma progression

JN Xiao, TH Yan, RM Yu, Y Gao, WL Zeng, SW Lu… - Journal of Cancer Research …, 2017

... Total of 5000 cells were seeded into 96-well plates and cultured overnight. Cell
proliferation abilities at 0, 24, 48, 72 h, after transfection were detected using Cell Counting
Kit-8 (Dojindo, Japan) and measured in the absorbance at 450 nm. ...

[HTML] Edaravone protects against oxygen-glucose-serum deprivation/restoration-induced apoptosis in spinal cord astrocytes by inhibiting integrated stress response

B Dai, T Yan, Y Shen, Y Xu, H Shen, D Chen, J Wang… - Neural Regeneration …, 2017

... supplied medium for 6 hours. Cell viability assay Cell Counting Kit-8 (CCK-8; Dojindo
Laboratories, Kumamoto, Japan) was used to measure cell viability, according to
the manufacturer's protocol. Cells were plated on 96-well ...

The study of mechanisms of biological activity of copper oxide nanoparticle CuO in the test for seedling roots of Triticum vulgare

AM Korotkova, SV Lebedev, IA Gavrish - Environmental Science and Pollution …, 2017

... Determination of viability and activity of reductase output formazan using WST-test (Cell
Counting Kit-8). ... The indicator is defined in the test (Cell Counting Kit-8) in the number
of water-soluble tetrazolium salt (WST) passed into formazan. ...

[PDF] miR-1181 inhibits invasion and proliferation via STAT3 in pancreatic cancer

J Wang, XJ Guo, YM Ding, JX Jiang - World J Gastroenterol, 2017

... We then investigated miR-1181's effect on invasion and proliferation of pancreatic cancer cells
by transwell assay, wound healing assay, cell counting kit-8 assay and colony-forming assay,
and ... We then added 10 µL of CCK-8 (Dojindo, Tokyo, Japan) solution to each well. ...

Controlled release of core-shell ZSM-5/chitosan ellipsoids loaded with pifithrin-a for enhanced osteoinductivity

R Zhu, YX Chen, QF Ke, CQ Zhang, YP Guo - Materials & Design, 2017

... 2.6. Cell proliferation. The proliferations of the hBMSCs on ZSM-5/CS and ZSM-5/CS/PFTa
ellipsoids were examined by Cell Counting Kit-8 assay (Dojindo Molecular Technologies, Inc.
Japan). Briefly, 1 × 10 4 hBMSCs were seeded on ZSM-5 and ZSM-5/CS, respectively. ...

[HTML] Nuclear retention of the lncRNA SNHG1 by doxorubicin attenuates hnRNPC–p53 protein interactions

Y Shen, S Liu, J Fan, Y Jin, B Tian, X Zheng, H Fu - EMBO reports, 2017

Skip to main content. Advertisement. ...

[PDF] Ginsenoside Rg1 ameliorates cigarette smoke-induced airway fibrosis by suppressing the TGF-ß1/Smad pathway in vivo and in vitro

S Guan, Q Liu, F Han, W Gu, L Song, Y Zhang, X Guo…

... for western blot analysis. 2.3. Cell viability assay CSE and ginsenoside Rg1
cytotoxicities were evaluated using a Cell Counting Kit-8 (CCK-8) assay (Dojindo,
Kumamoto, Japan). Ginsenoside Rg1 (20 mg) was dissolved in 20 mL of ...

Enhanced growth and osteogenic differentiation of MC3T3-E1 cells on Ti6Al4V alloys modified with reduced graphene oxide

X Li, K Linb, Z Wang

... Cell proliferation on the rGO coating To evaluate the effect of the rGO-coated titanium alloy
substrates on the proliferation of MC3T3-E1 cells, the Cell Counting Kit-8 (CCK-8) was
used according to the instructions (Dojindo, Kumamoto, Japan). ...

Osteoblast-like cell responses to ion products released from magnesium-and silicate-containing calcium carbonates

S Yamada, Y Ota, A Obata, T Kasuga - Bio-Medical Materials and Engineering, 2017

... The culture plates were placed in a CO 2 incubator for 3 hours. The adhered cellular
numbers in the culture well were estimated using a Cell Counting Kit-8 (CCK-8;
Dojindo, Kumamoto, Japan), following its instructions. The ...

BRCA1-Associated Protein-1 Suppresses Osteosarcoma Cell Proliferation and Migration Through Regulation PI3K/Akt Pathway

S Gao, H Sun, C Cheng, G Wang - DNA and Cell Biology, 2017

... Cell counting kit-8 assays for proliferation Around 3 ×103 cells per well were seeded in 96-well
plates, the viability of the cells was detected from three replicates by the Cell Counting Kit-8
(CCK-8) Assay Kit (Dojindo; CK04) at indicated time point following the manufacturer's ...

[PDF] A minicircuitry comprised of microRNA-9 and SIRT1 contributes to leukemogenesis in t (8; 21) acute myeloid leukemia

L Zhou, L Fu, N Lv, XS CHEN, J Liu, Y Li, QY XU… - European Review for …, 2017

... Cell Proliferation The number of viable cells was assessed using a CCK-8 (Cell
Counting Kit-8) assay (Dojindo, Kumamoto, Japan) according to the manufacturer's
instructions. After 6, 24, 48, or 72 h of transfection, cells were ...

[HTML] MicroRNA-320 inhibits invasion and induces apoptosis by targeting CRKL and inhibiting ERK and AKT signaling in gastric cancer cells

Y Zhao, Q Dong, E Wang - OncoTargets and therapy, 2017

... CCK8 assay. CCK8 assay was performed using Cell Counting Kit-8 solution (Dojindo,
Gaithersburg, MD, USA) according to the manufacturer's protocol. Cells were incubated with
10 µL/well of Cell Counting Kit-8 solution during the last 4 h of the culture. ...

[HTML] ß-Catenin promotes cell proliferation, migration, and invasion but induces apoptosis in renal cell carcinoma

C Yang, S Ji, Y Li, L Fu, T Jiang, F Meng - OncoTargets and therapy, 2017

... Cell proliferation assay. A498, 786-O, and ACHN cells were seeded in a 96-well plate (1×10
5 cells/well) and cultured at 37°C for indicated times. Cell proliferation was detected by Cell
Counting Kit-8 (CCK-8; Dojindo Molecular Technologies, Tokyo, Japan). ...

The soy-derived peptide Vglycin inhibits the growth of colon cancer cells in vitro and in vivo

C Gao, R Sun, YR Xie, AL Jiang, M Lin, M Li, ZW Chen… - Experimental Biology and …, 2017

Alkali-etching growth of nest-like Ag@ mTiO 2 hierarchical nanostructures and their potential applications

Z Zhang, H Zhang - Materials Science and Engineering: C, 2017

... Reagent Co., Ltd. Doxycycline (Doxy, 99.9%) was purchased from Beijing Huafeng
United Technology Co., Ltd. Cell Counting Kit-8 (CCK-8) was purchased from Dojindo
Molecular Technologies (Japan). For cell culture medium ...

Iriomoteolides-9a and 11a: two new odd-numbered macrolides from the marine dinoflagellate Amphidinium species

K Kumagai, M Tsuda, E Fukushi, J Kawabata… - Journal of Natural …, 2017

... containing 10% fetal calf serum. After 72 h, the number of viable cells was counted
using Cell Counting Kit-8 (Dojindo Co., Kumamoto, Japan), according to the
manufacturer's instructions. The assay reagent was a tetrazolium ...

[PDF] Incorporating isosorbide as the chain extender improves mechanical properties of linear biodegradable polyurethanes as potential bone regeneration materials

Y Ma, J Liu, M Luo, J Xing, J Wu, H Pan, C Ruan, Y Luo - RSC Advances, 2017

... Cell counting kit-8 (CCK-8) and 4,6- diamidino-2-phenylindole (DAPI) were purchased from
Dojindo (Kumamoto, Japan). Alkaline phosphatase assay kit and BCIP/ NBT ALP color
development kit were purchased from Beyo- time (Shanghai, China). ...

Inhibition of ALDH2 Protects PC12 Cells against Formaldehyde-Induced Cytotoxicity: involving the Protection of Hydrogen Sulfide

Y Chen, CF Zhou, F Xiao, HL Huang, P Zhang, HF Gu… - Clinical and Experimental …, 2017

... selective pharmacological pan-Trk inhibitor) were purchased from Sigma Chemical CO (St. Louis,
MO, USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies,
Inc. (Rockvile, MD, USA). Specific ALDH2 antibody were purchased from Abcam. ...

An in vitro investigation of the marked impact of dendritic cell interactions with bone grafts

L Zhang, J Ke, Y Wang, S Yang, RJ Miron, Y Zhang - Journal of Biomedical Materials …, 2017

... At time points 1, 3 and 5 days, cell number was quantified using a Cell Counting Kit-8
(Dojindo, Japan) according to the manufacturer's protocol and measured by a microplate
reader at 450 nm (PowerWave XS2, BioTek, Winooski, VT, USA). ...

[HTML] The protective effects of propofol against CoCl 2-induced HT22 cell hypoxia injury via PP2A/CAMKIIa/nNOS pathway

Y Lu, W Chen, C Lin, J Wang, M Zhu, J Chen, C Miao - BMC anesthesiology, 2017

... Analysis for cell viability. Cell viability was maesured by cell counting kit-8 (CCK8)
(Dojindo Laboratories, Kumamoto, Japan) according to the manufacture's instruction.
Briefly, 5 × 10 3 cells per well were plated in 96-well plates ...

Application of PEG and EGCG modified collagen-base membrane to promote osteoblasts proliferation

C Chenyu, J Deng, Y Hou, L Xiang, Y Wu, Y Qu, Y Man - Materials Science and …, 2017

... 2.5. Cell viability. The cell viability was determined by Cell Counting Kit-8 (CCK-8,
Dojindo Laboratories, Kumamoto, Japan). MG63 cells were cultured on collagen
membranes for 1, 2 and 3 days. The collagen membranes were ...

[PDF] Matrine inhibits TGF-ß1-induced proliferation and migration of airway smooth muscle cells by suppressing the MAPK signaling pathway

G Geng, H Si, G Mao, B Guan, Y Zhang, Y Zheng… - Int J Clin Exp Med, 2017

... Cell Counting kit-8 (CCK-8; Dojindo Molecul- ar Technologies, Inc., Kumamoto, Japan) was used
to assess the rate of cellular proliferation. Briefly, human ASMCs were plated into 96- well plates
at a seed density of ~2×103 cells/ well in 100 µl culture medium. ...

[HTML] Effects of Histone Deacetylase Inhibitor (Valproic Acid) on the Expression of Hypoxia-inducible Factor-1 Alpha in Human Retinal Müller Cells

YJ Kim, SJ Park, NR Kim, HS Chin - Korean Journal of Ophthalmology, 2017

... were exposed to 0, 100, 200, 300, 400, 500, 600, 700, 800, 900, or 1,000 µM CoCl 2 . The cells
were then washed twice with phosphate buffered saline and the cell counting kit-8 (CCK-8;
Dojindo Molecular Technologies, Rockville, MD, USA) was used to measure cell viability. ...

[PDF] MiR-630 acts as a tumor suppressor in cervical cancer and inhibits epithelial-mesenchymal transition in cervical cancer

RZ Jiang - Int J Clin Exp Pathol, 2017

... wells. Added Cell Counting Kit-8 (CCK-8, WST- 8, Dojindo, JaPan) to each well after 0
h, 24 h, 48 h, 72 h and 96 h respectively and incu- bated with 1 h, detected at 450 nm using
a microplate reader. The experiment was repeat- ed three times. ...

[PDF] Effect of calcitonin-gene-related peptide on proliferation and osteogenesis in rat bone mesenchymal stem cells

T Zhang, X Yu, S Liu, L Lv, Y Wang, S Li - Int J Clin Exp Pathol, 2017

... GAPDH protein ex- pression was used as an in- ternal control. Cell proliferation assay
The status of rBMSCs cell pro- liferation was evaluated us- ing Cell Counting Kit-8 (CCK-
8, Dojindo, Japan) according to the manufacturer's instr- uction. ...

[PDF] Long non-coding RNA XIST promotes cervical cancer cell epithelial-mesenchymal transition through the Wnt/ß-catenin pathway

G Sun, C Wang, H Zhang - Int J Clin Exp Pathol, 2017

... Page 2. XIST regulated EMT by Wnt/ß-catenin in CC 2334 Int J Clin Exp Pathol 2017;10(2):2333-
2339 Cell proliferation assay Cell proliferation was measured using a Cell Counting Kit-8
(CCK-8, Dojindo). Cells were seeded into 96-well plates at 2000 cells per well. ...

[HTML] Raf Kinase Inhibitor Protein Attenuates Ischemic-Induced Microglia Cell Apoptosis and Activation Through NF-?B Pathway

L Su, R Zhang, Y Chen, C Ma, Z Zhu - Cellular Physiology and Biochemistry, 2017

... Cell viability assay. Cell viability was assayed by Cell Counting Kit-8 according to the
manufacturer's instructions (Dojindo, Kumamoto, Japan). Lactate dehydrogenase (LDH) release
test was performed as instructed by the manufacturer (Promega, Madison, WI). ...

Radicol, a Novel Trinorguaiane-Type Sesquiterpene, Induces Temozolomide-Resistant Glioma Cell Apoptosis via ER Stress and Akt/mTOR Pathway Blockade

ZY Li, C Zhang, L Chen, BD Chen, QZ Li, XJ Zhang… - Phytotherapy Research, 2017

... to the resistant A172, U118, U251, U87 cell lines and the parental cell line was determined using
a Cell Counting Kit-8 (CCK-8) assay to ensure the TMZ resistance of the cells. Cell viability assay.
Cell viability was evaluated with a CCK-8 kit (Dojindo Molecular Technologies ...

Facile synthesis and characterization of novel rapid-setting spherical sub-micron bioactive glasses cements and their biocompatibility in vitro

Y Li, Q Liang, C Lin, X Li, X Chen, Q Hu - Materials Science and Engineering: C, 2017

... monolayer of cells in each well. Cell proliferation was measured using cell-counting
kit 8 (CCK-8) assays (Dojindo). The study was repeated three times and six replicates
were used within each study. In situ and real time cell ...

Astragaloside IV inhibits isoprenaline-induced cardiac fibrosis by targeting the reactive oxygen species/mitogen-activated protein kinase signaling axis

H Dai, G Jia, M Lu, C Liang, Y Wang, H Wang - Molecular Medicine Reports

... Following incubation for 24 h in a humidified atmosphere of 5% CO2, at 37°C, the supernatant
was removed, and 100 µl DMEM containing 10 µl Cell Counting Kit-8 (Dojindo Molecular
Technologies, Inc., Kumamoto, Japan) was added to each well for a further 4 h at 37°C. The ...

Reduced expression of SIRT2 in serous ovarian carcinoma promotes cell proliferation through disinhibition of CDK4 expression

Y Du, J Wu, H Zhang, S Li, H Sun - Molecular Medicine Reports

... Results were from three independent experiments performed in triplicate. Cell Counting Kit-8
assay. The experiment was performed according to the manufacturer's protocol (Cell Counting
Kit-8; cat. no. CK04; Dojindo Molecular Technologies, Inc., Kumamoto, Japan). ...

Protective effects of hydrogen sulfide against angiotensin II-induced endoplasmic reticulum stress in HUVECs

HJ Hu, ZS Jiang, J Qiu, SH Zhou, QM Liu - Molecular Medicine Reports

... no. 12032-1-AP). Cell Counting kit-8 (CCK-8) was obtained from Dojindo China Co., Ltd.
(Shanghai, China). Horseradish peroxidase-conjugated IgG (H+L) secondary antibodies were
purchased from Beyotime Institute of Biotechnology (Shanghai, China; cat. nos. ...

Response of human non-small-cell lung cancer cells to the influence of Wogonin with SGK1 dynamics

G Shi, Q Wang, X Zhou, J Li, H Liu, J Gu, H Wang… - Acta Biochimica et Biophysica …

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[HTML] RGD peptide and graphene oxide co-functionalized PLGA nanofiber scaffolds for vascular tissue engineering

YC Shin, J Kim, SE Kim, SJ Song, SW Hong, JW Oh… - Regenerative Biomaterials

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to use our website, you are agreeing to our use of cookies. You can change
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