Cytotoxicity Assay Kit-WST

∼ Feature ∼

  1. Can be used with and without transferring supernatant
  2. Stable Working Solution (6 months at 0-5 °C)
  3. No need to freeze the Working Solution

 

MSDS



Detection Principle

Lactate dehydrogenase(LDH) is an enzyme that presents in almost cell types and it catalyzes the oxidation of lactate to pyruvate in the presence of co-enzyme NAD+. Once cells are impaired by stress, injuries, chemicals, or intercellular signals, LDH is rapidly released from the cell membrane. Thus, the measurement of the amount of released LDH from cells is one of the major methods to assess the cell death. Since Dojindo's Cytotoxicity LDH Assay Kit-WST neither reflects the activity of living cells nor is harmful to cells, it allows the assay to perform in wells containing both viable and damaged cells.



Choose between Two Procedures

Cytotoxicity LDH Assay Kit-WST can be applied with and without supernatant transferring. Please choose suitable method for your experiment.




Stable Working Solution

Working Solution is stable for 6 months under refrigerated conditions. Therefore, after the preparation, Working Solution can be used as a ready-to-use solution at any time during this period.

Experimental Examples


To obtain an accurate result in cytotoxicity assay, the samples are measured by different principles. Cell Counting Kit-8 (Product Code: CK04) measure NADH in living cells and LDH assay measure released LDH from dead cells. According to these results, the living cells decreased and dead cells increased with higher concentration of toxicant.


Storage Condition: 0-5°C

Shipping Condition: ambient temperature

Required Equipment and Materials:
Microplate reader with 490 nm filter, CO2 incubator,
96-well tissue culture plate(flat bottomed), 20 μl, 100-200 μl multi-channel pipette


Kit Contents by Unit Size

Contents of the Kit: 100 tests
Dye Mixture :1 tube Assay Buffer :11 ml, 1 tube
Lysis Buffer :1.1 ml, 1 tube Stop Solution :5.5 ml, 1 tube


Contents of the Kit: 500 tests
Dye Mixture :1 tube Assay Buffer :55 ml, 1 tube
Lysis Buffer :5.5 ml, 1 tube Stop Solution :27.5 ml, 1 tube


Contents of the Kit: 2000 tests
Dye Mixture :4 tube Assay Buffer :55 ml, 4 tube
Lysis Buffer :5.5 ml, 4 tube Stop Solution :27.5 ml, 4 tube

1) S. F. Jin, H. L. Ma, Z. L. Liu, S. T. Fu, C. P Zhang, Y. He, "XL413, a cell division cycle 7 kinase inhibitor enhanced the anti-fibrotic effect of pirfenidone on TGF-β1-stimulated C3H10T1/2 cells via Smad2/4.", Exp Cell Res., 2015, 2015113000019.
2) S. Watanabe, C. S. Moniaga, S. Nielsen, M. Hara-Chikuma, "Aquaporin-9 facilitates membrane transport of hydrogen peroxide in mammalian cells.Aquaporin-9 facilitates membrane transport of hydrogen peroxide in mammalian cells.", Biochem Biophys Res Commun ., 2016, 471, 191.
3) L. Wu, T. Oshima, J. Shan, H. Sei, T. Tomita, Y. Ohda, H. Fukui, J. Watari, H. Miwa , "PAR-2 activation enhances weak acid-induced ATP release through TRPV1 and ASIC sensitization in human esophageal epithelial cells.", Am J Physiol Gastrointest Liver Physiol ., 2015, 309, G695.

4)T. Fukami, A. Iida, K. Konishi, M. Nakajima , "Human arylacetamide deacetylase hydrolyzes ketoconazole to trigger hepatocellular toxicity", Biochem. Pharmacol.., 2016, doi:10.1016/j.jphs.2016.07.007.

5) M. Tanaka, M. Yoneyama, T. Shiba, T. Yamaguchi, K. Ogita, "Protease-activated receptor-1 negatively regulates proliferation of neural stem/progenitor cells derived from the hippocampal dentate gyrus of the adult mouse ", J Pharmacol Sci., 2016, doi:10.1016/j.jphs.2016.05.005.
6) S. Wakatsuki, A. Furuno, M. Ohshima, and T. Araki, "Oxidative stress-dependent phosphorylation activates ZNRF1 to induce neuronal/axonal degeneration", J Cell Biol., 2015, 211, (4), 881.

Stability of Working Solution



Reconstituted Working Solution has higher stability than competitor's kit, eliminating the additional time to prepare the Working Solution for each assay.



Comparison of Working Solution Stability



Linearity



Dojindo's Cytotoxicity LDH Assay Kit-WST has higher sensitivity than competitor's kits



Cytotoxicity of mitomycin C using HeLa cells



Culture medium: MEM, 10% FBS


Incubation: 37C, 5% CO2, 48 hours

Price List

Item # Description/Size Availability Qty Break Price Quantity
CK12-01
100 tests
1-2 business days 1 $80.00
CK12-05
500 tests
1-2 business days 1 $198.00
CK12-20
2000 tests
1-2 business days 1 $398.00

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For Research Use Only Products