SAT-3 | Dojindo

Home > Analytical / Biological Products > Diagnostic Analysis > Hydrogen Peroxide / Peroxidase Detection >

SAT-3

Zoom

Item #	Description/Size	Availability	Qty Break	Price	Quantity
S302-10	1 g	5-10 business days	1	$180.00

*Estimated. Exact shipping date will be notified.
For Research Use Only Products

Application: Peroxidase detection

Description
Application

Appearance: White or pale-brown powder
Purity: ≥95.0% (HPLC)
MW: 604.62, C₂₀H₂₆N₂Na₂O₈S₂• 4H₂O

Storage Condition: 0-5ºC, protect from light
Shipping Condition: ambient temperature

Oxidation Reaction

Product Description

SAT-3 is a stable, highly water-soluble o-Tolidine analog. It is easily oxidized by peroxidase and hydrogen peroxide into a green dye at pH 4-6. While TMBZ requires an organic solvent or detergent to solubilize, SAT-3 can be solubilized with just buffer solution. The SAT-3 solution can be stored at room temperature with protection from light. The maximum wavelength of the dye is 675 nm. Using the Ames test, Nno mutagenicity of SAT-3 was detected. using the Ames test.

Assay Protocol
1. Mix 1 ml of 100 mM SAT-3 solution and 1 ml of 30 mM hydrogen peroxide solution with 8 ml of 50 mM citrate buffer (pH 4) to prepare an assay solution.
2. Add 100-200 μl of the assay solution to each well of a 96-well plate and incubate at 37ºC for 5-30 minutes.
3. Read the O.D. at 670 nm or add 50 μl of 4 M sulfuric acid to each well and read the O.D. at 490 nm instead.

Reference
M. Mizoguchi, et al., Sensitive Chromogenic Substrate for Detecting Peroxidase Activity. Anal Commun. 1998;35:179.

Related Items: