DAB | Dojindo

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DAB

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Item #	Description/Size	Availability	Qty Break	Price	Quantity
D006-10	1 g	5-10 business days	1	$156.00
D006-12	5 g	5-10 business days	1	$582.00

*Estimated. Exact shipping date will be notified.
For Research Use Only Products

Description
Application

Chemical Name: 3,3’-Diaminobenzidine, tetrahydrochloride
CAS: 7411-49-6

Appearance: White or slightly pinkish-gray powder
Purity: ≥97% (Titration)
MW: 360.11, C₁₂H₁₈Cl₄N₄

Storage Condition : 0-5ºC, protect from light
Shipping Condition: Ambient Temperature

Product Description
DAB is one of the most commonly used oxidative chromogenic dyes for the detection of peroxidase in immunohistochemistry. In the presence of H₂O₂ and peroxidase, DAB is oxidized into a brown pigment. These brown pigments are aniline black type compounds and are firmly deposited around peroxidase on cell membranes or tissues. Most DAB available on the market appears brown as a result of oxidation during processing or storage. On the other hand, Dojindo offers a high quality DAB with a white or slightly reddish-gray powder appearance that is good for sharp staining.

Preparation of Sample Staining Solution
1. Dissolve 9 mg DAB with 1 ml PBS to prepare 100X DAB solution.
2. Mix 5 μl of 30% hydrogen peroxidase solution with 1 ml PBS to prepare 200X H₂O₂ solution.
3. Add 10 ul of 100X DAB solution and 5 ul of 200X H₂O₂ solution to 1 ml PBS to prepare staining solution^a).
4. Add the staining solution to a sample in a staining chamber, and incubate the sample at room temperature for 5 minutes to 1 hour ^b).
5. Wash the sample with PBS several times to stop the staining reaction.

^a)The staining solution is not stable. Prepare fresh solution prior to use.
^b)For better staining, incubate the sample with 0.09 mg DAB/PBS solution for 10 minutes before adding the staining solution.

References

1. A. B. Novikoff, et al., Studies on Microperoxisomes V. Are Microperoxisomes Ubiquitous in Mammalian Cells. J Histochem Cytochem. 1973;21:737.
2. V. Herzog, et al., A New Sensitive Colorimetric Assay for Peroxidaase Using 3, 3’-Diaminobenzidine as Hydrogen Donor. Anal Biochem. 1973;55:554.
3. H. Yamada, et al., Improvement of Technique of Immunohistochemical Demonstraction of Bioactive Substances in the Central Nervous System. Acta Histochem Cytochem. 1987;20:629.
4. K. L. Cheng, Determination of Traces of Selenium 3,3’-Diaminobenzidine as Selenium(IV) Organic Reagent. Anal Chem. 1956;28:1738.

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