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Home > Surface Modification > PEG Alkanethiol >
Amino-EG6-hexadecanethiol, hydrochloride
*Estimated. Exact shipping date will be notified.
For Research Use Only Products
Application: SAM preparation, amine group coating,
and hydrophilic surface
Chemical Name: 20-(16-Mercaptohexadecanyloxy)-3,6,9,12,15,18-hexaoxaeicosane-1-amine, hydrochloride
Appearance: White or slightly yellow solid
Purity: ≥95.0% (HPLC)
Solubility:10 mmol/L (Ethyl alcohol, Methyl alcohol, Chloroform)
MW: 574.30, C28H60ClNO6S
Storage Condition: -20oC, protect from light and metal
Shipping Condition: ambient temperature
Structural Formula
Product Description
Polyethyleneglycols
(PEGs) are widely used for material modifications to improve
hydrophilicity of the surface. PEG-coated materials are usually more
stable under physiological conditions. Since Amino-EG6-undecanethiol
has 6 ethylene glycol units, 11 carbon atoms, and an SH group at the
end, it can be used to prepare a highly oriented and hydrophilic SAM on a
gold surface. This is suitable for biomaterial labeling on the surface
due to the improved hydrophilicity. The hydrophilic surface can prevent
proteins or other biomaterials from non-specific binding. Therefore, the
SAM prepared by this reagent will provide a better surface to develop
biomaterial sensors or DNA/ protein microarrays. To prepare an Amino-EG6-SAM
on a gold surface, hydroxy-EGn-undecanethiols (n=3, 6) are used to
dilute the number of amino groups according to the density of the
molecules being introduced onto the surface. How to Prepare SAM1. Soak a gold-coated glass plate in Piranha solutiona) for 10-15 minutes. Wash the plate with purified water.a)2. Dissolve aminoalkanethiol compound in ethanol to prepare several mM to several ten mM solutions.3. Soak the plate in the aminoalkanethiol solution for a certain time period.b)4. Wash the SAM-coated plate with ethanol and then water.5. Dry the plate under nitrogen atmosphere, if necessary.a)Piranha
solution: sulfuric acid and 30% hydrogen peroxide, 3:1. Piranha
solution is a strong oxidizing agent. Extreme care is necessary when
using it.
Do not apply Piranha solution to resin-coated plates; it may erode the resin.
b)To
prepare a SAM-coated plate with the best performance, aminoalkanethiol
concentration and soaking time should be individually determined.
Application of SAM-Preparation of DNA Array (Fig. 1)1. Use SF10 glass slides (Schott Glass Technologies) coated with 5 nm chromium and 45 nm gold thin film.2. Soak the glass slide in a 1 mM 1-octadecanethiol (ODT)/ethanol solution overnight to prepare ODT SAM-coated slide.3. Draw 500 μm x 500 μm patterns on the ODT SAM-coated slide by UV irradiation with an Hg-Xe arc lamp.a)4.
Soak the slide in a 1 mM 11-amino-1-undecanethiol (AUT)/ethanol
solution for 2 hours to form AUT SAM on the 500 μm x 500 μm
photopatterned area.5. Drop 2 mM SPDP solutionb) onto the slide and leave the slide at room temperature. 6. Wash the slide and dry under nitrogen atmosphere.7. Apply 1 mM thiol-DNA solutionc) to each 500 μm x 500 μm pattern and incubate at room temperature overnight.8. Incubate the slide with a sample solution for 10 minutes and wash with phosphate buffer, followed by SPR imaging. a)Irradiation time: 1-1.5 hours
b)SPDP:
N-succinimidyl 3-(2-pyridyldithio)propionate. Dissolve SPDP in DMSO to
prepare 50 mM solution. Dilute it 25 times with 100 mM triethanolamine
buffer, pH 7.0.
c)Dissolve thiol-DNA with 100 mM triethanolamine buffer, pH 8.0.
Fig. 1 DNA Array Preparation Scheme
References
1. C. Pale-Grosdemange, E. S. Simon, K. L. Prime, and G. M. Whitesides, Anal. Chem., 1999, 71, 777-790.
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