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Biotin-SAM Formation Reagent
*Estimated. Exact shipping date will be notified.
For Research Use Only Products
Simple preparation of Biotin-SAM on gold surfaceEfficient Immobilization of AvidinsSuppress unspecific binding with unknown proteins
Appearance: White or slightly yellowish solid
Storage Condition: 0-5°C
Shipping Condition: ambient temperature
Keyword: Self-Assembled Monolayers, SAM, SPR, QCM, biosensors, protein immobilization
Product Description
There are three ways to immobilize a protein on an Surface Plasmon Resonance (SPR) or Quartz Crystal Microbalance (QCM) biosensor consisting of Self-Assembled Monolayer (SAM); 1) Formation of covalent bond of amine functions on proteins and activated carboxy-SAM; 2) immobilize proteins by His-Tag through Ni-NTA-terminated SAM; 3) Iimmobilization of proteins through Biotin and Avidin interaction. Because the Biotin-Avidin formation is known as facile reaction, it is widely utilized for immobilization of protein (Fig 1).
Fig. 2 Comparison of the amount of bound streptavidin, unspecific binding of FBS, bound biotinylated BSA between Biotin-SAM Formation Reagent and Company A.