Chemical Name: 4,4 EDicarboxy-2,2 Ebiquinoline, disodium salt
Appearance: Slightly yellow or yellow powder
Molar absorptivity: ≥15,500 (around 332 nm)
MW: 388.28, C20H10N2Na2O4
Storage Condition: ambient temperature, protect from metal
Shipping Condition: ambient temperature
Sodium bicinchoninate is a Cu(I)- specific colorimetric reagent that is more sensitive than neocuproine. Its absorption maximum is at 562 nm (molar absorptivity: 7,900). In alkaline solution, Cu(II) is reduced by proteins to Cu(I), and then forms a purple complex with Sodium bicinchoninate. The protein detection range of this assay is 1-2,000 mg per ml.Chemical Structure
1. K. Ueno, et al., Colloid Titration - A Rapid Method for the Determination of Charged Colloid. J Chem Educ. 1985;62:627-629.
2. P. K. Smith, et al., Measurement of Protein Using Bicinchoninic Acid. Anal Biochem. 1985;150:76-85.
3. M. G. Redinbaugh, et al., Adaptation of the Bicinchoninic Acid Protein Assay for Use with Microtiter Plates and Sucrose Gradient Fractions. Anal Biochem. 1986;153:267-271.