ChemicalName:2-Hydroxy-1-(2-hydroxy-4-sulfo-1-naphthylazo)-3,6-naphthalendisulfonic acid, trisodium salt Purity: pass test
Appearance: Black purple powder
Absorbance (Ca complex): ≥0.310 (around 562 nm)
MW: 620.48, C20H11N2Na3O11S3
Storage Condition: ambient temperature, protect from metal
Shipping Condition: ambient temperature
Product DescriptionHNB is a metal indicator for calcium, and a colorimetric reagent for alkaline earth metal ions, rare earth metal ions, and uranium ions. HNB is readily soluble in water and alcohol. Aqueous solution of HNB is blue at pH 7-12, red above pH 13, and pink in the presence of calcium at pH 10. The detection range of alkaline earth metal ions and rare earth metal ions are 1-600 ppm and 1-300 ppm, respectively. The maximum wavelength of the complex is 650 nm. HNB is also used for UO2(II) detection. The proton dissociation constants of HNB are reported to be pKa4=6.44 and pKa5=12.91(μ=0.1, 24ºC).Chemical Structure References
1. A. Itoh, et al., Evaluation of 2-Hydroxy-1-(2-Hydroxy-4-Sulpho-1-Naphthylazo)-3-Naphthoic Acid and Hydroxynaphthol Blue as Metallochromic Indicators in the EDTA Titration of Calcium. Analyst. 1970;95:583-589.
2. M. Sugawara, et al., Catalytic Decomposition of the Excess Reagent in the Spectrophotometric Determination of Copper(II) with O,O EDihydroxyazo Compounds. Bull Chem Soc Jpn. 1977;50:3206-3208.
3. M. M. Ferris, et al., Examination of Metallochromic Indicators and Water-soluble Reagents for Metals by Planar Electrophoresis. Analyst. 1986;111:351-354.