ChemicalName: Mixture of monopyrazolone and bisbyrazolone (12.5:1)
Appearance: White or pale yellow powder
Sensitivity: To pass test
Storage Condition: ambient temperature
Shipping Condition: ambient temperature
Cyanoline Blue is a mixture of monopyrazolone and bispyrazolone. It is used as a colorimetric reagent for CN- detection. The detection range of CN- by this method is 5 ppb to 10 ppm. The maximum wavelength is 620 nm. A ready-to-use solution can be prepared by dissolving 0.27 g Cyanoline Blue in 20 ml pyridine, followed by dilution with 100 ml of water. The solution is stable for one week at 4 oC. Cyanoline Blue is also used to detect SCN-, OCN-, NH3, and vitamin B12.
1. J. M. Kruse, et al., Colorimetric Determination of Cyanide and Thiocyanate. Anal Chem. 1953;25:446-450.
2. L. Prochazkova, Spectrophotometric Determination of Ammonia as Rubazoic Acid with Bispyrazolone Reagent. Anal Chem. 1964;36:865-871.
3. S. Baar, The Micro Determination of Cyanide: Its Application to the Analysis of Whole Blood. Analyst. 1966;91:268-272.