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Home > Analytical / Biological Products > Ion Detections >
*Estimated. Exact shipping date will be notified.
For Research Use Only Products
Application: Metal indicator, Ca2+ and Mg2+ detection, colorimetric
Chemical Name: 2-Hydroxy-1-(1-hydroxy-2-naphthylazo)-6-nitro-4-naphthalenesulfonic acid, sodium salt
CAS: 1787-61-7
Appearance: Black or blackish brown powder
Absorbance in borate buffer(1 mg / 100 ml, pH9): ≥0.400(around 620 nm)
MW: 461.38, C20H12N3NaO7S
Storage Condition : ambient temperature
Shipping Condition : ambient temperature
Product Description
BT in aqueous solution changes color according to pH: red at pH<6, blue at pH 7-11, and orange at pH>12. Its proton dissociation constants are reported to be pKa2=6.3 and pKa3=11.55 (m=0.08, 20ºC). BT is one of the most commonly used indicators of Ca, Mg, Zn, Cd, Pb, Hg, and rare earth metals for EDTA chelate titration. BT solution turns red in the presence of the metals at pH 10, and then changes to blue at the endpoint of the titration. Cu and Fe(III), as well as Al, Co, and Mn(III), should be masked with KCN because they interfere with the color change. Triethanolamine is also a suitable masking reagent for Al, Fe(III), and Mn(III). Since metal ions such as Mn(III) and Fe(III) oxidize BT, hydroxylamine and hydrochloride should be added to prevent oxidation.
Chemical Structure
References
1. P. F. Lott, et al., Spectrophotometric Determination of Thorium with Eriochrome Black T. Anal Chem. 1960;32:1702-1704.
Table 1 Metal Detection Conditions
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All Products are applicable only for life science research. Not for diagnostic research use.
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