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MDB

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Item #	Description/Size	Availability	Qty Break	Price	Quantity
M021-10	50 mg	5-10 business days	1	$91.00
M021-12	100 mg	5-10 business days	1	$133.00

*Estimated. Exact shipping date will be notified.
For Research Use Only Products

Description
Application

Chemical Name: 1,2-Diamino-4,5-methylenedioxybenzene, dihydrochloride
CAS: 38608-07-0

Appearance: White or slightly pink crystalline powder
Purity: ≥98.0% (Titration)
MW: 225.07, C₇H₁₀Cl₂N₂O₂

Storage Condition: -20°C, protect from moisture
Shipping Condition: blue ice

Derivatization Reaction

Product Description
Phenylenediamine (OPD) reacts with α-keto acids to form highly fluorescent quinoxaline derivatives. MDB is an o-phenylenediamine analogue. It is the best labeling reagent for α-keto acids because of its reactivity and sensitivity. In HPLC analysis, more than ten different α-keto acids, such as α-keto glutaric acid, pyruvic acid, and phydroxyphenyl-pyruvic acid, can be separately detected at a time, even at concentrations of only a few femtomoles per injection. The sensitivity of MDB is almost 150 times as high as OPD. The excitation and emission wavelength of the labeled materials are 367 nm and 445 nm, respectively.

References
1. M. Nakamura, et al., 1, 2-Diamino-4, 5-methylenedioxybenzene as a Highly Sensitive Fluorogenic Reagent for α-Keto Acids. Chem Pharm Bull. 1987;35:687-692.
2. S. Hara, et al., Fluorometric High-Performance Liquid Chromatography of N-Acetyl-and N-Glycolylneuraminic Acids and Its Application to Their Microdetermination in Human and Animal Sera, Glycoproteins, and Glycolipids. Anal Biochem. 1987;164:138-145.
3. S. Hara, et al., 1, 2-Diamino-4, 5-methylenedioxybenzene as a Highly Sensitive Fluorogenic Reagent for α-Dicarbonyl Compounds. Anal Chim Acta. 1988;215:267-276.